1. Home
  2. Full-length cDNAs
  3. RAFL02-06-F22

RAFL02-06-F22

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00045)

full reading [+] show sequence

Nucleotide sequence: RAFL02-06-F22 - full reading 
ACCAGAAGAA GAGCCACACA CTCACAAATT AAAAAGAGAG AGAGAGAGAG 
AGAGACAGAG AGAGAGAGAG ATTCTGCGGA GGAGCTTCTT CTTCGTAGGG 
TGTTCATCGT TATTAACGTT ATCGCCCCTA CGTCAGCTCC ATCTCCAGAA 
ACATGGGTGC AGGTGGAAGA ATGCCGGTTC CTACTTCTTC CAAGAAATCG 
GAAACCGACA CCACAAAGCG TGTGCCGTGC GAGAAACCGC CTTTCTCGGT 
GGGAGATCTG AAGAAAGCAA TCCCGCCGCA TTGTTTCAAA CGCTCAATCC 
CTCGCTCTTT CTCCTACCTT ATCAGTGACA TCATTATAGC CTCATGCTTC 
TACTACGTCG CCACCAATTA CTTCTCTCTC CTCCCTCAGC CTCTCTCTTA 
CTTGGCTTGG CCACTCTATT GGGCCTGTCA AGGCTGTGTC CTAACTGGTA 
TCTGGGTCAT AGCCCACGAA TGCGGTCACC ACGCATTCAG CGACTACCAA 
TGGCTGGATG ACACAGTTGG TCTTATCTTC CATTCCTTCC TCCTCGTCCC 
TTACTTCTCC TGGAAGTATA GTCATCGCCG TCACCATTCC AACACTGGAT 
CCCTCGAAAG AGATGAAGTA TTTGTCCCAA AGCAGAAATC AGCAATCAAG 
TGGTACGGGA AATACCTCAA CAACCCTCTT GGACGCATCA TGATGTTAAC 
CGTCCAGTTT GTCCTCGGGT GGCCCTTGTA CTTAGCCTTT AACGTCTCTG 
GCAGACCGTA TGACGGGTTC GCTTGCCATT TCTTCCCCAA CGCTCCCATC 
TACAATGACC GAGAACGCCT CCAGATATAC CTCTCTGATG CGGGTATTCT 
AGCCGTCTGT TTTGGTCTTT ACCGTTACGC TGCTGCACAA GGGATGGCCT 
CGATGATCTG CCTCTACGGA GTACCGCTTC TGATAGTGAA TGCGTTCCTC 
GTCTTGATCA CTTACTTGCA GCACACTCAT CCCTCGTTGC CTCACTACGA 
TTCATCAGAG TGGGACTGGC TCAGGGGAGC TTTGGCTACC GTAGACAGAG 
ACTACGGAAT CTTGAACAAG GTGTTCCACA ACATTACAGA CACACACGTG 
GCTCATCACC TGTTCTCGAC AATGCCGCAT TATAACGCAA TGGAAGCTAC 
AAAGGCGATA AAGCCAATTC TGGGAGACTA TTACCAGTTC GATGGAACAC 
CGTGGTATGT AGCGATGTAT AGGGAGGCAA AGGAGTGTAT CTATGTAGAA 
CCGGACAGGG AAGGTGACAA GAAAGGTGTG TACTGGTACA ACAATAAGTT 
ATGAGGATGA TGGTGAAGAA ATTGTCGACC TTTCTCTTGT CTGTTTGTCT 
TTTGTTAAAG AAGCTATGCT TCGTTTTAAT AATCTTATTG TCCATTTTGT 
TGTGTTATGA CATTTTGGCT GCTCATTATG TTATGTGGGA AGTTAGTGTT 
C

Gene models with high sequence identity

  1. AT3G12120.1 [+] show detail - fatty acid desaturase 2
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Fatty acid desaturase, type 1 2.6e-33
HMMPfam Protein of unknown function DUF3474 0.00000046

See the detailed result >

AT3G12120.1

Model type
Protein coding
Short Description
fatty acid desaturase 2
Curator Summary
Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
Computational Description
fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL02-06-F22 introduced

  • F00650 - seed::decreased size / seed::decreased length
  • F03619 - Specific phenotypes were NOT observed.
  • F03621 - Specific phenotypes were NOT observed.
  • F07721 - Specific phenotypes were NOT observed.
  • F07929 - Specific phenotypes were NOT observed.
  • F10231 - Specific phenotypes were NOT observed.
  • F10521 - seed::increased size / seed::increased length
  • F10802 - Specific phenotypes were NOT observed.
  • F13801 - Specific phenotypes were NOT observed.
  • F14606 - Specific phenotypes were NOT observed.
  • F24121 - Specific phenotypes were NOT observed.
  • F27208 - Specific phenotypes were NOT observed.
  • F27405 - Specific phenotypes were NOT observed.
  • F30119 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

23 mutant lines found