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RAFL04-17-I16

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87414)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL04-17-I16 - 5 prime EST 
GATTTCTCTG AAAACCCTTA AATCTCCGGA TTTCGATTCC CGAGGCTCGA 
AAAAACCCTA AATCTCTTCT CCGTTCACTC ATCCAACCGA AGATGCGTGA 
GATTCTTCAC ATCCAGGGNG GTCAATGCTG GTAACCAGAT CGGCGCCAAG 
TTCTGGGAAG TGGTTTGCGC CGAGCACGGN ATCGATCCAA CCGGAAGGTA 
CACCGGAGAC TCAGATCTGC AACTTGAGCG CATCAACGTT TACTACAATG 
AAGCGAGTTG CGGTAGATTC GTTCCTCGTG CAGTGCTCAT GGATTTGGAG 
CCTGGGACTA TGTATAGTCT CAGATCTGGA CCGTACGGTC AGACCTTTCG 
ACCTGATAAC TTCGTCTTTG GCCAATCCGG TGCTGGTAAC AACTGGGCCA 
AGGGACACCT ACACGGAAGG AGCTGAACTA ATCGATTCCG TTCTCGATGT 
TGTTCGTAAG GAAGCTGANA ACTGTGGACT GTCTCCAAGG GTTCCAGGTT 
GGTCA

Gene models with high sequence identity

  1. AT5G62690.1 [+] show detail - tubulin beta chain 2
    E-value: 0; Score: 98.41

3 prime EST [+] show sequence

Nucleotide sequence: RAFL04-17-I16 - 3 prime EST 
AACCAAACGC TTCTCTATTT TATTCATTAC AGAACCGCAC TTAACATATG 
AAAATAGGGT TCACTGCACC AGATCATTAC CCACTAATCT CCTCTTAAAG 
ACCTTGACAA AATTACATGT AATAATAATC AATCACTGCT TGTTGTTCAA 
GAGAGCTGCT CGTAGCTCAG CCGCGTCAAT CTCGTTGGAA CCGGTTTCGG 
GTCTGTAATA ACCGGTTTTG GGATCTGGAA CCCAAGAAAT CTTCTGGGTT 
GATTCTTCCA CTCCCTTCTT CTTCATCACA GCCGAAGCAA CAGCTCCACT 
TCTTCCACCG CTCGAAACGC TCCCTTGCGC CGCCGTGGCC GCATAACCAC 
GTCGGAAGAT AGCATTGGAG AGTTCACGAG AGACGAAAGC AGATACGATC 
TTAACGTTAG AGATAGAACG AGCCATTTTC GAAGTAAGTG GTTTCTTGAA 
AAGAGAGAAA ACAGACTTTT TATTCTTG

Gene models with high sequence identity

  1. AT4G02380.1 [+] show detail - senescence-associated gene 21
    E-value: 0; Score: 100.00

AT4G02380.1

Model type
Protein coding
Short Description
senescence-associated gene 21
Curator Summary
Encodes AtLEA5 (late embryogenesis abundant like protein). Also known as SENESCENCE-ASSOCIATED GENE 21 (SAG21). Has a role on oxidative stress tolerance. mRNA levels are elevated in response to various stresses.
Computational Description
senescence-associated gene 21 (SAG21); CONTAINS InterPro DOMAIN/s: Late embryogenesis abundant protein, group 3 (InterPro:IPR004926); BEST Arabidopsis thaliana protein match is: Late embryogenesis abundant 3 (LEA3) family protein (TAIR:AT1G02820.1); Has 181 Blast hits to 176 proteins in 36 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 181; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G62690.1

Model type
Protein coding
Short Description
tubulin beta chain 2
Curator Summary
encodes tubulin beta-2/beta-3 chain
Computational Description
tubulin beta chain 2 (TUB2); FUNCTIONS IN: structural molecule activity, GTP binding, GTPase activity; INVOLVED IN: response to cadmium ion, response to salt stress, response to cold; LOCATED IN: tubulin complex, nucleolus, cell wall, plasma membrane; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Beta tubulin (InterPro:IPR002453), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Beta tubulin, autoregulation binding site (InterPro:IPR013838), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin beta chain 3 (TAIR:AT5G62700.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-17-I16 introduced

  • F19746 - Specific phenotypes were NOT observed.
  • F27032 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

7 mutant lines found

6 mutant lines found