1. Home
  2. Full-length cDNAs
  3. RAFL04-17-O24

RAFL04-17-O24

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00651)

full reading [+] show sequence

Nucleotide sequence: RAFL04-17-O24 - full reading 
ATTTGCTCAC CTTTCGCCGA TTGATTTTGT ACATCACACG CAGACGCAGC 
TTTTAGTATT TTTTTCAACA ATCGCTTTTT TTTTTTTCTC GATCTCCGTG 
AAGCTCTCGA ATCTCTGGTT TTGAGTTTTG GAGAATGGTG TTTTTCCGCA 
GCGTATCGGC CTTTACTAGG CTGAGGTCTC GCGTCGGCCA ACAATCTTCA 
CTCAGCAATT CTGTCAGATG GATTCAGATG CAGAGCTCTA CCGACCTGGA 
CCTGAAGTCG CAGCTGCAAG AGTTAATTCC GGAACAGCAG GACCGTCTGA 
AGAAACTGAA GTCAGAACAT GGGAAGGTCC AACTGGGAAA CATCACTGTT 
GATATGGTTA TTGGTGGAAT GAGAGGGATG ACTGGATTGC TTTGGGAAAC 
CTCATTGCTT GACCCGGAAG AGGGTATTCG CTTTAGGGGA TTGTCGATTC 
CTGAGTGCCA GAAAGTATTA CCTACTGCCC AGTCTGGAGC TGAACCATTA 
CCGGAGGGTC TTTTGTGGCT TCTCTTAACT GGAAAGGTAC CTAGCAAAGA 
GCAAGTTGAA GCACTGTCGA AAGACTTGGC GAACCGTGCT GCTGTGCCAG 
ATTATGTGTA CAATGCCATC GATGCTCTGC CTTCCACAGC TCATCCAATG 
ACTCAATTTG CTAGCGGTGT TATGGCCCTC CAGGTGCAAA GTGAGTTTCA 
AAAGGCATAT GAGAATGGAA TTCATAAGTC AAAGTTCTGG GAGCCAACAT 
ATGAGGATTG CCTCAACCTG ATTGCTCGTG TTCCTGTTGT AGCTGCATAT 
GTTTATCGAA GGATGTATAA GAATGGTGAT TCCATTCCCT CAGATAAATC 
TTTGGATTAT GGTGCAAATT TTTCCCACAT GTTGGGATTT GATGATGAAA 
AGGTGAAAGA GCTCATGAGG CTTTACATCA CCATCCACAG TGATCATGAA 
GGTGGAAATG TTAGTGCTCA CACTGGTCAC CTGGTTGGTA GTGCACTTTC 
AGACCCATAT CTGTCATTTG CAGCTGCATT AAATGGTTTA GCTGGGCCAC 
TCCATGGTTT GGCTAATCAG GAAGTTTTGC TTTGGATCAA ATCAGTCGTA 
GAGGAATGTG GAGAAGATAT ATCAAAAGAA CAGTTGAAAG AATATGTTTG 
GAAAACATTA AACAGTGGCA AGGTTATTCC GGGATATGGT CACGGTGTTC 
TGCGCAATAC TGATCCAAGA TATGTATGCC AAAGAGAATT TGCCTTGAAG 
CATCTACCTG ACGACCCTCT TTTCCAGCTG GTGTCAAAGC TTTATGAAGT 
TGTGCCTCCT GTTCTCACTG AGCTTGGAAA GGTGAAGAAC CCGTGGCCAA 
ATGTTGATGC TCACAGTGGG GTCTTGCTGA ACCACTATGG TCTAACCGAA 
GCAAGGTACT ACACCGTGCT CTTTGGTGTT TCAAGGAGTC TTGGCATCTG 
CTCTCAGCTT ATATGGGACC GAGCTCTTGG ACTTGCACTT GAGAGGCCAA 
AGAGTGTTAC CATGGACTGG CTTGAAGCCC ATTGTAAGAA AGCTTCATCT 
GCTTAAGTCA CCACCGAGTC CTGAGTTGTC GCACTCTCTC GTGTTTCAAC 
AAAAGTCATT CTCTTCCACA CTGGATAGCT TTTTTTGTTT TTACCTTCTA 
TTGACAGAAG AAACATTTGT TCATGTAGAA ATTGTTCCTG AGAAACACAT 
GCTTGGTGCA AATTTGCATT TCCGGGAAAC TCGTGATAAT AAATAAACAG 
TTGTATT

Gene models with high sequence identity

  1. AT2G44350.1 [+] show detail - Citrate synthase family protein
    E-value: 0; Score: 99.89

 

InterPro Scan Digest

Program Description E-value
HMMTigr Citrate synthase, eukaryotic 0
superfamily Citrate synthase-like, core 2e-145
Gene3D Citrate synthase-like, large alpha subdomain 9.1e-101
PatternScan Citrate synthase active site 0
HMMPanther Citrate synthase-like 5.4e-217
FPrintScan Citrate synthase-like 1.2e-53
FPrintScan Citrate synthase-like 1.2e-53
FPrintScan Citrate synthase-like 1.2e-53
FPrintScan Citrate synthase-like 1.2e-53
FPrintScan Citrate synthase-like 1.2e-53
FPrintScan Citrate synthase-like 1.2e-53
HMMPfam Citrate synthase-like 1.2e-106
Coil coiled-coil 0

See the detailed result >

AT2G44350.1

Model type
Protein coding
Short Description
Citrate synthase family protein
Curator Summary
encodes a mitochrondrion targeted citrate synthase, the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA.
Computational Description
ATCS; FUNCTIONS IN: citrate (SI)-synthase activity, zinc ion binding, ATP binding; INVOLVED IN: response to cadmium ion, tricarboxylic acid cycle; LOCATED IN: mitochondrion, cell wall, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Citrate synthase-like, large alpha subdomain (InterPro:IPR016142), Citrate synthase, eukaryotic (InterPro:IPR010109), Citrate synthase active site (InterPro:IPR019810), Citrate synthase-like (InterPro:IPR002020), Citrate synthase-like, core (InterPro:IPR016141); BEST Arabidopsis thaliana protein match is: citrate synthase 5 (TAIR:AT3G60100.1); Has 10534 Blast hits to 10528 proteins in 2768 species: Archae - 158; Bacteria - 6911; Metazoa - 258; Fungi - 320; Plants - 178; Viruses - 0; Other Eukaryotes - 2709 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-17-O24 introduced

  • F05850 - whole plant::decreased height / sporophyte vegetative stage::decreased speed / stem internode::decreased length / fruit::abnormal
  • F15845 - stem::abnormal / stem internode::decreased length / stem::abnormal
  • F21933 - stem::abnormal / rosette leaf::abnormal / cauline leaf::increased size / whole plant::decreased height / stem::abnormal / stem internode::decreased length
  • F25829 - Specific phenotypes were NOT observed.
  • F26046 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

5 mutant lines found