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  3. RAFL04-17-P12

RAFL04-17-P12

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00555)

full reading [+] show sequence

Nucleotide sequence: RAFL04-17-P12 - full reading 
TGGCACACAC TTCTCTCTCT CTCTCTCTCT GCCTCCTTTC GGATTCAAAT 
CTCAGATCTA GCTCAACCAT GGCGTTGCTC GTCGAGAAGA CCTCAAGTGG 
CCGTGAATAC AAGGTCAAAG ACATGTCTCA AGCCGATTTC GGTCGTCTCG 
AACTCGAGCT CGCCGAAGTT GAGATGCCTG GACTCATGGC TTGTCGTACC 
GAATTCGGAC CTTCTCAGCC ATTCAAAGGC GCTAGAATCA CCGGATCTCT 
TCACATGACC ATCCAAACCG CCGTACTCAT CGAAACCCTA ACTGCTCTCG 
GTGCTGAAGT CAGATGGTGT TCCTGCAACA TCTTTTCCAC TCAAGACCAC 
GCCGCCGCAG CCATCGCTCG TGACTCCGCC GCTGTTTTCG CCTGGAAAGG 
TGAGACTCTT CAGGAGTACT GGTGGTGTAC CGAGCGTGCT CTAGATTGGG 
GTCCAGGTGG TGGTCCTGAT CTGATTGTTG ATGATGGTGG TGACGCTACT 
CTTTTGATTC ATGAGGGTGT TAAAGCTGAG GAGATCTTTG AGAAGACTGG 
TCAAGTTCCT GATCCTACTT CTACTGATAA CCCTGAGTTT CAGATCGTGT 
TGTCTATTAT CAAGGAAGGT CTTCAAGTTG ATCCTAAGAA GTACCACAAG 
ATGAAGGAGA GACTTGTTGG TGTCTCTGAG GAAACTACCA CTGGTGTTAA 
GAGGCTTTAC CAGATGCAGC AAAATGGAAC TCTTTTGTTC CCTGCCATTA 
ACGTTAACGA CTCTGTCACC AAGAGCAAGT TCGACAACTT GTATGGTTGC 
CGTCACTCAC TCCCTGATGG TCTCATGAGG GCCACTGATG TCATGATCGC 
TGGAAAGGTT GCTGTTATCT GTGGATATGG TGATGTTGGA AAGGGTTGTG 
CTGCTGCCAT GAAGACTGCT GGTGCTAGAG TCATTGTGAC TGAGATTGAT 
CCCATCTGTG CCCTTCAAGC TTTGATGGAA GGACTTCAGG TTCTTACCCT 
TGAGGATGTT GTCTCAGAAG CTGATATCTT TGTCACCACC ACCGGTAACA 
AAGACATCAT CATGGTCGAC CACATGAGGA AGATGAAGAA CAACGCTATT 
GTGTGCAACA TTGGTCACTT TGACAATGAG ATTGACATGC TTGGACTTGA 
GACTTACCCT GGTGTGAAGC GTATCACCAT CAAGCCACAG ACTGACAGGT 
GGGTGTTCCC AGAGACCAAG GCTGGAATCA TTGTCTTGGC TGAGGGTCGT 
CTGATGAACT TGGGTTGTGC CACTGGTCAC CCAAGTTTCG TGATGTCTTG 
CTCTTTCACC AACCAGGTGA TTGCCCAGCT CGAGCTCTGG AACGAGAAAG 
CAAGCGGAAA GTACGAGAAG AAGGTGTACG TTCTTCCCAA GCATTTGGAT 
GAGAAGGTTG CATTACTTCA CTTGGGCAAG CTTGGAGCCA GGCTTACAAA 
GCTGTCAAAG GACCAATCTG ACTACGTCAG CATTCCAATT GAGGGACCAT 
ACAAGCCTCC TCACTACAGG TACTGAGAAG AGAGAGAGAG TCGACAAAGC 
GGTTCAGGTT CGGATCTACT TGTGGTTTTG TGTTGGGTTG TGGTGGGAGA 
GTGGAACAGT TTGAGATATT GGTCTTCTGA TGAAGTTGAC CAAATATCAG 
TATTAATAAG GGTTATTGGC TTTTGAAGGT TGTGCTTGTT TTCTCCATTT 
TTCATGAAAC TTAAATTAGT TTTTGGTAT

Gene models with high sequence identity

  1. AT4G13940.1 [+] show detail - S-adenosyl-L-homocysteine hydrolase
    E-value: 0; Score: 99.94

 

InterPro Scan Digest

Program Description E-value
HMMPIR S-adenosyl-L-homocysteine hydrolase 2.1e-252
HMMPanther S-adenosyl-L-homocysteine hydrolase 0
PatternScan S-adenosyl-L-homocysteine hydrolase, conserved site 0
PatternScan S-adenosyl-L-homocysteine hydrolase, conserved site 0
HMMPfam S-adenosyl-L-homocysteine hydrolase 2.9e-143
HMMPfam S-adenosyl-L-homocysteine hydrolase, NAD binding 7.8e-84
Gene3D no description 7.3e-134
superfamily Formate/glycerate dehydrogenase catalytic dom 7.1e-114
superfamily NAD(P)-binding domain 7.8e-45
HMMTigr S-adenosyl-L-homocysteine hydrolase 8.6e-238

See the detailed result >

AT4G13940.1

Model type
Protein coding
Short Description
S-adenosyl-L-homocysteine hydrolase
Curator Summary
Encodes a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing.
Computational Description
MATERNAL EFFECT EMBRYO ARREST 58 (MEE58); FUNCTIONS IN: adenosylhomocysteinase activity, copper ion binding; INVOLVED IN: methylation-dependent chromatin silencing, one-carbon metabolic process, posttranscriptional gene silencing, embryo development ending in seed dormancy; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 36 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: S-adenosyl-L-homocysteine hydrolase, conserved site (InterPro:IPR020082), S-adenosyl-L-homocysteine hydrolase (InterPro:IPR000043), S-adenosyl-L-homocysteine hydrolase, NAD binding (InterPro:IPR015878); BEST Arabidopsis thaliana protein match is: S-adenosyl-l-homocysteine (SAH) hydrolase 2 (TAIR:AT3G23810.1); Has 7964 Blast hits to 7958 proteins in 1477 species: Archae - 229; Bacteria - 2334; Metazoa - 639; Fungi - 145; Plants - 247; Viruses - 0; Other Eukaryotes - 4370 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-17-P12 introduced

  • F28618 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

3 mutant lines found