1. Home
  2. Full-length cDNAs
  3. RAFL04-18-J05

RAFL04-18-J05

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87425)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL04-18-J05 - 5 prime EST 
TGACTCCCCG TCCTACAGGT CGACTCAGTC TCCGGCTAGT TCGACTCGGT 
AAAATCACGA TTACTTTCTC CTTCCATTTA TCACGATCGT GGATCCAGAA 
ACTCTGAAAG ATGGCGTCTG AGCTGACAAA CCGGCGACAC GAGATAGAGC 
AACCAGAAGC GGAATCTTAT TATCCAAAGC CGATAAAGCC ATGGTTCGTA 
GCGATTCGTC CGATCCGTTA CATGCTCAGA GAACAGAGAC TCGTCTTCGT 
CCTCGTCGGC ATTGCCATCG CCACATTAGG ATTCACAATC TTCTCCAAAT 
CATCCAACCA CCAACCAATT CCTTACGACG TGGATCCGCT TTCCGGATAC 
GGAATGCGAT CGGAGTCATC GTATCTGCCA GCGACGATTC ATAAGAAGCC 
AAGCATCGAG TACATGAGCC GGATCGGATC GGCAGGCGGC AAAATCCCTC 
TAGGCTTGAA ACGGAAAGTG CTAAGGGTTG TTGTGACAGA TGGAGCAGGG 
TTCGTCGGGT CACACCTGGT GGACCGGTTG ATGGCTCGAG GAGACAACGT 
GATCGTGGTC GACAATTTCT TCACGGGAAG GAAAGAGAAC GTGATGCACC 
ATTTCAATAA CCCTAATTTC GAGATGATCC GTCACGATGT AGTGGAGCCG 
ATCCTTCTAG ANGTTGATCA GATCTACCAT TTGG

Gene models with high sequence identity

  1. AT2G47650.2 [+] show detail - UDP-xylose synthase 4
    E-value: 0; Score: 99.71

3 prime EST [+] show sequence

Nucleotide sequence: RAFL04-18-J05 - 3 prime EST 
GGCTCAAAAC TACGGTGATT GCATTAAAAA AAATAAAACT TTAAAATCGG 
TGAGGTTTCA ATATCACTAC ATTGACAGGA ATAGAAATAT CAAAATTAAA 
AATGAAAACG AAGAGAAACG AGAATCTCCA GCGACAGTTA CTCTGTTGAT 
GATGAAGTGG TGGAAGAATC CTGCTTTTGG TCGCCAAAGA CACGTTGACG 
GAAATCTTTG ACCATAAGTG GGAGTCCTTG GCGTAGAGCC ACCTTTGGTT 
CCCAACCAAG AAGCTCTTTG GCCTTTGTGA TGTCTGGTTT CCTCTTGTGT 
GGGTCGTCCT CTGTGTTTGG TCTGAACTCT ATCTTTGCGT TCGGGTCAAT 
TGTCTCTTGA ACCACCTTAG CGAGCTCGAG CATGGTGAAT TCACCGGGGT 
TACCAAGGTT AAATGGACCG ACATGTTCTC CCTCCATCAA CCTCATCAGA 
CCTTCCACCA GATCAGAGAC AAACTGGAAA CTCCTAGTCT GCTTACCATC 
ACCATAAACA GTCAAAGGCT CTTTTCTTAA TGCCTGGGCA ACGAAGTTGC 
TAACCACACG ACCGTCATCG ATACACATCC TTGGGCCATA AGTGTTGAAT 
ATCCTAGCAA TCCTAACCTC AACATTAGCG CCTCTGTGAT AGTCCATGGT 
CAAAGTCTCA GCCGTACGTT TGCCTTCGTC GTAACACCT

Gene models with high sequence identity

  1. AT2G47650.1 [+] show detail - UDP-xylose synthase 4
    E-value: 0; Score: 100.00

AT2G47650.1

Model type
Protein coding
Short Description
UDP-xylose synthase 4
Curator Summary
encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
Computational Description
UDP-xylose synthase 4 (UXS4); FUNCTIONS IN: UDP-glucuronate decarboxylase activity, catalytic activity; INVOLVED IN: nucleotide-sugar metabolic process, D-xylose metabolic process; LOCATED IN: Golgi apparatus, plasma membrane, vacuole, membrane; EXPRESSED IN: cotyledon, male gametophyte, cultured cell, pollen tube, leaf; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2); Has 44652 Blast hits to 44610 proteins in 2978 species: Archae - 851; Bacteria - 26010; Metazoa - 746; Fungi - 333; Plants - 1297; Viruses - 49; Other Eukaryotes - 15366 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G47650.2

Model type
Protein coding
Short Description
UDP-xylose synthase 4
Curator Summary
encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
Computational Description
UDP-xylose synthase 4 (UXS4); CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-18-J05 introduced

  • F07226 - Specific phenotypes were NOT observed.
  • F19738 - rosette leaf::increased width / rosette leaf::abnormal / cauline leaf::increased size / stem::decreased thickness / stem::abnormal

Mutant lines with disrupted or induced

14 mutant lines found