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RAFL04-18-J05

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87425)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL04-18-J05 - 5 prime EST
TGACTCCCCG TCCTACAGGT CGACTCAGTC TCCGGCTAGT TCGACTCGGT 
AAAATCACGA TTACTTTCTC CTTCCATTTA TCACGATCGT GGATCCAGAA
ACTCTGAAAG ATGGCGTCTG AGCTGACAAA CCGGCGACAC GAGATAGAGC
AACCAGAAGC GGAATCTTAT TATCCAAAGC CGATAAAGCC ATGGTTCGTA
GCGATTCGTC CGATCCGTTA CATGCTCAGA GAACAGAGAC TCGTCTTCGT
CCTCGTCGGC ATTGCCATCG CCACATTAGG ATTCACAATC TTCTCCAAAT
CATCCAACCA CCAACCAATT CCTTACGACG TGGATCCGCT TTCCGGATAC
GGAATGCGAT CGGAGTCATC GTATCTGCCA GCGACGATTC ATAAGAAGCC
AAGCATCGAG TACATGAGCC GGATCGGATC GGCAGGCGGC AAAATCCCTC
TAGGCTTGAA ACGGAAAGTG CTAAGGGTTG TTGTGACAGA TGGAGCAGGG
TTCGTCGGGT CACACCTGGT GGACCGGTTG ATGGCTCGAG GAGACAACGT
GATCGTGGTC GACAATTTCT TCACGGGAAG GAAAGAGAAC GTGATGCACC
ATTTCAATAA CCCTAATTTC GAGATGATCC GTCACGATGT AGTGGAGCCG
ATCCTTCTAG ANGTTGATCA GATCTACCAT TTGG

Gene models with high sequence identity

  1. AT2G47650.2 [+] show detail - UDP-xylose synthase 4
    E-value: 0; Score: 99.71

3 prime EST [+] show sequence

Nucleotide sequence: RAFL04-18-J05 - 3 prime EST
GGCTCAAAAC TACGGTGATT GCATTAAAAA AAATAAAACT TTAAAATCGG 
TGAGGTTTCA ATATCACTAC ATTGACAGGA ATAGAAATAT CAAAATTAAA
AATGAAAACG AAGAGAAACG AGAATCTCCA GCGACAGTTA CTCTGTTGAT
GATGAAGTGG TGGAAGAATC CTGCTTTTGG TCGCCAAAGA CACGTTGACG
GAAATCTTTG ACCATAAGTG GGAGTCCTTG GCGTAGAGCC ACCTTTGGTT
CCCAACCAAG AAGCTCTTTG GCCTTTGTGA TGTCTGGTTT CCTCTTGTGT
GGGTCGTCCT CTGTGTTTGG TCTGAACTCT ATCTTTGCGT TCGGGTCAAT
TGTCTCTTGA ACCACCTTAG CGAGCTCGAG CATGGTGAAT TCACCGGGGT
TACCAAGGTT AAATGGACCG ACATGTTCTC CCTCCATCAA CCTCATCAGA
CCTTCCACCA GATCAGAGAC AAACTGGAAA CTCCTAGTCT GCTTACCATC
ACCATAAACA GTCAAAGGCT CTTTTCTTAA TGCCTGGGCA ACGAAGTTGC
TAACCACACG ACCGTCATCG ATACACATCC TTGGGCCATA AGTGTTGAAT
ATCCTAGCAA TCCTAACCTC AACATTAGCG CCTCTGTGAT AGTCCATGGT
CAAAGTCTCA GCCGTACGTT TGCCTTCGTC GTAACACCT

Gene models with high sequence identity

  1. AT2G47650.1 [+] show detail - UDP-xylose synthase 4
    E-value: 0; Score: 100.00

AT2G47650.1

Model type
Protein coding
Short Description
UDP-xylose synthase 4
Curator Summary
encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
Computational Description
UDP-xylose synthase 4 (UXS4); FUNCTIONS IN: UDP-glucuronate decarboxylase activity, catalytic activity; INVOLVED IN: nucleotide-sugar metabolic process, D-xylose metabolic process; LOCATED IN: Golgi apparatus, plasma membrane, vacuole, membrane; EXPRESSED IN: cotyledon, male gametophyte, cultured cell, pollen tube, leaf; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2); Has 44652 Blast hits to 44610 proteins in 2978 species: Archae - 851; Bacteria - 26010; Metazoa - 746; Fungi - 333; Plants - 1297; Viruses - 49; Other Eukaryotes - 15366 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G47650.2

Model type
Protein coding
Short Description
UDP-xylose synthase 4
Curator Summary
encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
Computational Description
UDP-xylose synthase 4 (UXS4); CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-18-J05 introduced

  • F07226 - Specific phenotypes were NOT observed.
  • F19738 - rosette leaf::increased width / rosette leaf::abnormal / cauline leaf::increased size / stem::decreased thickness / stem::abnormal

Mutant lines with disrupted or induced

14 mutant lines found