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  3. RAFL04-19-J21

RAFL04-19-J21

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00667)

full reading [+] show sequence

Nucleotide sequence: RAFL04-19-J21 - full reading 
ATCATCATCT TCTTCTTCAA TGGCCTTCAC CGCTTTTCTC CTCCTCCTCT 
CTTCCATCGC CGCCGGCTTC CTCCTCCTAC TCCGCCGTAC ACGTTACCGT 
CGGATGGGTC TGCCTCCGGG AAGCCTTGGT CTCCCTCTGA TAGGAGAGAC 
TTTTCAGCTG ATCGGAGCTT ACAAAACAGA GAACCCTGAG CCTTTCATCG 
ACGAGAGAGT AGCCCGGTAC GGTTCGGTTT TCATGACGCA TCTTTTTGGT 
GAACCGACGA TTTTCTCAGC TGACCCGGAA ACGAACCGGT TTGTTCTTCA 
GAACGAAGGG AAGCTTTTTG AGTGTTCTTA TCCTGCTTCC ATTTGTAACC 
TTTTGGGGAA ACACTCTCTG CTTCTTATGA AAGGTTCTTT GCATAAACGT 
ATGCACTCTC TCACCATGAG CTTTGCTAAT TCTTCAATCA TTAAAGACCA 
TCTCATGCTT GATATTGACC GGTTAGTCCG GTTTAATCTT GATTCTTGGT 
CTTCTCGTGT TCTCCTCATG GAAGAAGCCA AAAAGATAAC GTTTGAGCTA 
ACGGTGAAGC AGTTGATGAG CTTTGATCCA GGGGAATGGA GTGAGAGTTT 
AAGGAAAGAG TATCTTCTTG TCATCGAAGG CTTCTTCTCT CTTCCTCTCC 
CTCTCTTCTC CACCACTTAC CGCAAAGCCA TCCAAGCGCG GAGGAAGGTG 
GCGGAGGCGT TGACGGTGGT GGTGATGAAA AGGAGGGAGG AGGAGGAAGA 
AGGAGCGGAG AGAAAGAAAG ATATGCTTGC GGCGTTGCTT GCGGCGGATG 
ATGGATTTTC CGATGAAGAG ATTGTTGACT TCTTGGTGGC TTTACTTGTC 
GCCGGTTATG AAACAACCTC CACGATCATG ACTCTCGCCG TCAAATTTCT 
CACCGAGACT CCTTTAGCTC TTGCTCAACT CAAGGAAGAG CATGAAAAGA 
TTAGGGCAAT GAAGAGTGAT TCGTATAGTC TTGAATGGAG TGATTACAAG 
TCAATGCCAT TCACACAATG TGTGGTTAAT GAGACGCTAC GAGTGGCTAA 
CATCATCGGC GGTGTTTTCA GACGTGCAAT GACGGATGTT GAGATCAAAG 
GTTATAAAAT TCCAAAAGGG TGGAAAGTAT TCTCATCGTT TAGAGCGGTT 
CATTTAGACC CAAACCACTT CAAAGATGCT CGCACTTTCA ACCCTTGGAG 
ATGGCAGAGC AACTCGGTAA CGACAGGCCC TTCTAATGTG TTCACACCGT 
TTGGTGGAGG GCCAAGGCTA TGTCCCGGTT ACGAGCTGGC TAGGGTTGCA 
CTCTCTGTTT TCCTTCACCG CCTAGTGACA GGCTTCAGTT GGGTTCCTGC 
AGAGCAAGAC AAGCTGGTTT TCTTTCCAAC TACAAGAACG CAGAAACGGT 
ACCCGATCTT CGTGAAGCGC CGTGATTTTG CTACTTGAAG AAGAAGAGAC 
CCATCTGATT TTATTTATAG AACAACAGTA TTTTTCAGGA TTAATTTCTT 
CTTCTTTTTT TGCCTCCTTG TGGGTCTAGT GTTTGACAAT AAAAGTTATC 
ATTACTCTAT AAAGCCTTAG CTTCTGTGTA CATAAAAAAA AAAAACTTTT 
GTTTACCTTA TGCTTGCATA AATCTCTTCT GCTTCAATGG T

Gene models with high sequence identity

  1. AT5G05690.1 [+] show detail - Cytochrome P450 superfamily protein
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Cytochrome P450 1.4e-61
HMMPanther CYTOCHROME P450 90A1 (STEROID 22 ALPHA-HYDROX 2.1e-247
HMMPanther Cytochrome P450 2.1e-247
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, E-class, group I 2.7e-18
FPrintScan Cytochrome P450, C-terminal 0.00000000091
FPrintScan Cytochrome P450, C-terminal 0.00000000091
FPrintScan Cytochrome P450, C-terminal 0.00000000091
FPrintScan Cytochrome P450, C-terminal 0.00000000091
PatternScan Cytochrome P450, conserved site 0
Gene3D Cytochrome P450 1.5e-92
superfamily Cytochrome P450 2.6e-96

See the detailed result >

AT5G05690.1

Model type
Protein coding
Short Description
Cytochrome P450 superfamily protein
Curator Summary
Encodes a member of the CP90A family, a cytochrome P450 monooxygenase which converts 6-deoxocathasterone to 6-deoxoteasterone in the late C6 oxidation pathway and cathasterone to teasterone in the early C6 oxidation pathway of brassinolide biosynthesis. Expressed in cotyledons and leaves. Mutants display de-etiolation and derepression of light-induced genes in the dark, dwarfism, male sterility and activation of stress-regulated genes in the light. The expression of the gene using a CPD promoter:LUC fusion construct was shown to be under circadian and light control. Additionally, the circadian regulation was shown to be independent of BR levels as it remains unchanged in bri1 mutant lines. CPD appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through a BRI1-mediated signaling pathway that affects FLC expression levels, as uncovered by double mutant analyses.
Computational Description
CONSTITUTIVE PHOTOMORPHOGENIC DWARF (CPD); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; INVOLVED IN: in 9 processes; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT3G50660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL04-19-J21 introduced

  • F25020 - rosette leaf::increased width / rosette leaf::present in fewer numbers in organism / whole plant::decreased height / stem::present in fewer numbers in organism
  • F26350 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

4 mutant lines found