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RAFL05-03-G03

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00687)

full reading [+] show sequence

Nucleotide sequence: RAFL05-03-G03 - full reading 
TGCTCTCTCG ATTGACTTTA TTGACTGAGA GAAAGCTTTT GACAATGGGC 
CACAGACACA GCAAGTCCAA ATCCTCCGAT CCGCCACCGT CCTCCTCCTC 
CTCTTCCTCC GGCAACGTAG TCCACCATGT TAAACCTGCC GGAGAACGCC 
GCGGATCATC CGGTTCCGGA ACCGTCGGAT CGTCAGGCTC CGGAACAGGC 
GGATCTCGTT CCACCACATC TACTCAGCAG AATGGTCGGA TCTTAGGTCG 
GCCGATGGAG GAAGTACGGA GAACCTATGA GTTTGGTCGT GAGCTTGGTC 
GTGGCCAGTT TGGTGTCACG TACCTCGTAA CTCACAAAGA GACGAAACAA 
CAGGTCGCTT GCAAGTCAAT CCCTACGCGC CGCCTTGTTC ACAAAGACGA 
CATTGAAGAT GTTCGCCGTG AAGTCCAGAT TATGCACCAT CTCAGCGGTC 
ACCGGAACAT AGTGGACTTG AAGGGAGCCT ACGAGGACAG ACACTCTGTG 
AATCTGATAA TGGAGTTGTG TGAAGGAGGG GAATTGTTCG ATAGGATCAT 
TTCTAAAGGT CTTTACTCAG AGAGAGCTGC TGCGGATTTG TGTAGGCAGA 
TGGTGATGGT TGTGCATAGT TGTCATTCTA TGGGTGTAAT GCACCGAGAC 
TTGAAGCCCG AAAACTTTCT CTTTCTTAGT AAAGATGAGA ACTCACCATT 
GAAAGCTACA GACTTTGGTC TCTCTGTCTT CTTCAAGCCA GGTGATAAGT 
TTAAGGATCT TGTTGGAAGT GCATACTATG TTGCCCCAGA AGTTCTAAAA 
CGGAACTATG GACCAGAGGC TGATATCTGG AGTGCTGGTG TGATTCTATA 
CATCCTTCTC AGTGGTGTTC CGCCTTTCTG GGGAGAAAAT GAAACGGGGA 
TCTTTGATGC CATTCTTCAA GGGCAACTTG ATTTTTCAGC TGATCCATGG 
CCAGCACTAT CAGATGGTGC CAAAGATCTT GTGAGGAAAA TGTTAAAATA 
TGACCCTAAA GATCGGCTTA CAGCTGCGGA AGTGCTAAAT CATCCGTGGA 
TTAGAGAAGA TGGGGAGGCA TCAGACAAAC CGCTTGACAA TGCGGTGTTA 
TCCAGGATGA AACAATTCCG GGCGATGAAC AAACTAAAAA AAATGGCCCT 
GAAGGTTATC GCTGAGAACC TTTCTGAAGA AGAAATCATT GGTCTTAAAG 
AGATGTTCAA ATCTCTAGAC ACTGATAACA ATGGAATAGT CACCTTGGAG 
GAATTGAGAA CCGGTCTCCC AAAACTTGGT TCAAAGATCT CTGAAGCTGA 
AATAAGGCAG TTAATGGAAG CAGCTGATAT GGATGGAGAT GGATCAATTG 
ATTACTTGGA GTTTATATCA GCCACAATGC ATATGAACAG AATCGAACGT 
GAGGATCACT TGTACACCGC ATTCCAGTTC TTTGACAATG ACAACAGCGG 
CTACATAACA ATGGAAGAGC TAGAGCTAGC CATGAAGAAA TACAACATGG 
GTGATGATAA ATCCATCAAA GAGATCATTG CTGAAGTAGA CACCGATCGT 
GACGGAAAAA TCAACTACGA AGAATTTGTA GCGATGATGA AGAAGGGAAA 
TCCAGAATTG GTGCCAAACC GACGCAGAAT GTGAGAGATC TTTTGATGTT 
GTTTGTTTGT TTTTGGTTAT CACTTTACTC AAACTTTGTG TTTCTGTTAC 
AATCAATTAT TTTGTTTCCT GC

Gene models with high sequence identity

  1. AT4G23650.1 [+] show detail - calcium-dependent protein kinase 6
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
ProfileScan Protein kinase, catalytic domain 48.612
ProfileScan EF-HAND 2 13.9
ProfileScan EF-HAND 2 11.64
ProfileScan EF-HAND 2 14.513
ProfileScan EF-HAND 2 10.915
superfamily Protein kinase-like domain 1.2e-91
superfamily EF-hand 2.3e-70
HMMPanther Calcium-dependent protein kinase 1.5e-273
HMMPanther Calcium/calmodulin-dependent protein kinase-like 1.5e-273
HMMSmart Serine/threonine-protein kinase domain 1.7e-102
HMMSmart Tyrosine-protein kinase, catalytic domain 3.9e-11
HMMSmart Calcium-binding EF-hand 0.00011
HMMSmart Calcium-binding EF-hand 0.0075
HMMSmart Calcium-binding EF-hand 0.081
HMMSmart Calcium-binding EF-hand 1.6e-07
Gene3D no description 1.3e-24
Gene3D no description 2.9e-60
Gene3D EF-hand-like domain 1.3e-39
PatternScan EF-Hand 1, calcium-binding site 0
PatternScan EF-Hand 1, calcium-binding site 0
PatternScan EF-Hand 1, calcium-binding site 0
PatternScan EF-Hand 1, calcium-binding site 0
PatternScan Protein kinase, ATP binding site 0
PatternScan Serine/threonine-protein kinase, active site 0
HMMPfam Serine/threonine-protein kinase-like domain 5.7e-71
HMMPfam EF-hand 3.6e-06
HMMPfam EF-hand 2e-09

See the detailed result >

AT4G23650.1

Model type
Protein coding
Short Description
calcium-dependent protein kinase 6
Curator Summary
Encodes calcium dependent protein kinase 3 (CPK3), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK3 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. CPK6 is also a member of the Arabidopsis CDPK family.
Computational Description
calcium-dependent protein kinase 6 (CDPK6); FUNCTIONS IN: calmodulin-dependent protein kinase activity, protein kinase activity, kinase activity; INVOLVED IN: in 6 processes; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 17 (TAIR:AT5G12180.1); Has 137650 Blast hits to 128872 proteins in 4025 species: Archae - 155; Bacteria - 13983; Metazoa - 52031; Fungi - 17582; Plants - 29890; Viruses - 500; Other Eukaryotes - 23509 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-03-G03 introduced

  • F03708 - Specific phenotypes were NOT observed.
  • F04705 - seed::increased size
  • F08414 - Specific phenotypes were NOT observed.
  • F15220 - Specific phenotypes were NOT observed.
  • F15835 - Specific phenotypes were NOT observed.
  • F23032 - rosette leaf::abnormal / fruit::decreased length / stem::abnormal
  • F26433 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

7 mutant lines found