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  3. RAFL05-05-M23

RAFL05-05-M23

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda01064)

full reading [+] show sequence

Nucleotide sequence: RAFL05-05-M23 - full reading 
ATTGTCGTCG GAGAAGGTGA AACAGCTCCG GTTGGTGCTG CGATTGGATT 
GTTAGCTGAG ACTGAAGCTG AGATCGAAGA AGCTAAGAGT AAAGCCGCTT 
CGAAATCTTC TTCTTCTGTG GCTGAGGCTG TCGTTCCATC TCCTCCTCCG 
GTTACTTCTT CTCCTGCTCC GGCGATTGCT CAACCGGCTC CGGTGACGGC 
AGTATCAGAT GGTCCGAGGA AGACTGTTGC GACGCCGTAT GCTAAGAAGC 
TTGCTAAACA ACACAAGGTT GATATTGAAT CCGTTGCTGG AACTGGACCA 
TTCGGTAGGA TTACGGCTTC TGATGTGGAG ACGGCGGCTG GAATTGCTCC 
GTCCAAATCC TCCATCGCAC CACCGCCTCC TCCTCCACCT CCGGTGACGG 
CTAAAGCAAC CACCACTAAT TTGCCTCCTC TGTTACCTGA TTCAAGCATT 
GTTCCTTTCA CAGCAATGCA ATCTGCAGTA TCTAAGAACA TGATTGAGAG 
TCTCTCTGTT CCTACATTCC GTGTTGGTTA TCCTGTGAAC ACTGACGCTC 
TTGATGCACT TTACGAGAAG GTGAAGCCAA AGGGTGTAAC AATGACAGCT 
TTATTAGCTA AAGCTGCAGG GATGGCCTTG GCTCAGCATC CTGTGGTGAA 
CGCTAGCTGC AAAGACGGGA AGAGTTTTAG TTACAATAGT AGCATTAACA 
TTGCAGTGGC GGTTGCTATC AATGGTGGCC TGATTACGCC TGTTCTACAA 
GATGCAGATA AGTTGGATTT GTACTTGTTA TCTCAAAAAT GGAAAGAGCT 
GGTGGGGAAA GCTAGAAGCA AGCAACTTCA ACCCCATGAA TACAACTCTG 
GAACTTTTAC TTTATCGAAT CTCGGTATGT TTGGAGTGGA TAGATTTGAC 
GCTATTCTTC CGCCAGGACA GGGTGCTATT ATGGCTGTTG GAGCGTCAAA 
GCCAACTGTA GTTGCTGATA AGGATGGATT CTTCAGTGTA AAAAACACAA 
TGCTGGTGAA TGTGACTGCA GATCATCGCA TTGTGTATGG AGCTGACTTG 
GCTGCTTTTC TCCAAACCTT TGCAAAGATC ATTGAGAATC CAGATAGTTT 
GACCTTATAA GACGCCAAGC GAAGACGAGA AGTCAAAAAC AGTTTCCAAA 
ATTCCTGAGC CAAATTTTTC CCAAGTAAAT TTTTTAATCT TCATTGTTCT 
TGGTCTTGCT CTACTTCTTT TGCATCTTTT TCTTCACTTG TGTTGTATCT 
GTATTTTTGT TTTCAAGAAT CATCATTTTG GGTTTTAAAC AAAAAAAAAA 
AAAAAAAAAA AAAAAAAAAA ACAAAAAAAA AAAAAAAA

Gene models with high sequence identity

  1. AT3G25860.1 [+] show detail - 2-oxoacid dehydrogenases acyltransferase family protein
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPanther PTHR23151 0
Gene3D E3 binding 0.0000489999951782359
Gene3D G3DSA:3.30.559.10 2.30000440726539e-60
HMMPfam 2-oxoacid dehydrogenase acyltransferase, catalytic domain 5.30000000000001e-64
superfamily SSF52777 1.2e-66
superfamily E3 binding 0.000000000062
HMMPfam E3 binding 0.000000000026
HMMPanther PTHR23151:SF9 0

See the detailed result >

AT3G25860.1

Model type
Protein coding
Short Description
2-oxoacid dehydrogenases acyltransferase family protein
Curator Summary
Nuclear encoded dihydrolipoamide S-acetyltransferase (LTA2) that encodes teh Pyruvate Decarboxylase E2 subunit. Mutant has embryo defect.
Computational Description
LTA2; FUNCTIONS IN: dihydrolipoyllysine-residue acetyltransferase activity; INVOLVED IN: metabolic process, acetyl-CoA biosynthetic process from pyruvate; LOCATED IN: cytosolic ribosome, chloroplast stroma, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: 2-oxo acid dehydrogenase, lipoyl-binding site (InterPro:IPR003016), E3 binding (InterPro:IPR004167), 2-oxoacid dehydrogenase acyltransferase, catalytic domain (InterPro:IPR001078), Single hybrid motif (InterPro:IPR011053), Biotin/lipoyl attachment (InterPro:IPR000089); BEST Arabidopsis thaliana protein match is: 2-oxoacid dehydrogenases acyltransferase family protein (TAIR:AT1G34430.1); Has 23844 Blast hits to 20819 proteins in 2344 species: Archae - 101; Bacteria - 13163; Metazoa - 1146; Fungi - 799; Plants - 467; Viruses - 32; Other Eukaryotes - 8136 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-05-M23 introduced

  • F13219 - rosette leaf::dark green / rosette leaf::decreased width / cauline leaf::decreased size / cauline leaf::desaturated green / cauline leaf::abnormal / stem::present in fewer numbers in organism / stem::abnormal / stem internode::decreased length / fruit::abnormal

Mutant lines with disrupted or induced

1 mutant line founds