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  3. RAFL05-10-E23

RAFL05-10-E23

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda00913)

full reading [+] show sequence

Nucleotide sequence: RAFL05-10-E23 - full reading 
AGGTGCCTCA AAAAATCATA ATTTTCTCGG AAAATTAAAA ATCATGTTTA 
AGATTTTCTC GAGAAAGTAA AAAAAGGAAG AAGAAGAAGA AGAAACCATG 
GGAAGAGCTT TCGTGTATGT AATTCTCGGA GGAGGTGTTG CTGCTGGTTA 
CGCAGCTCTC GAATTCACCC GTCGAGGTGT CTCCGATGGC GAACTCTGTA 
TCATTTCCGA AGAACCCGTT GCACCGTATG AGAGGCCTGC CTTAAGCAAA 
GGTTTTCTGC TGCCAGAAGC TCCTGCACGG CTTCCTTCTT TCCACACTTG 
TGTTGGAGCT AATGATGAGA AGCTTACACC GAAATGGTAC AAGGATCATG 
GAATTGAATT GGTACTTGGA ACTCGTGTTA AGTCCGTTGA TGTGAGGAGG 
AAGACTCTTT TATCGAGCAC AGGGGAGACT ATAAGTTACA AATTCTTGAT 
CATTGCAACA GGTGCCCGGG CGTTGAAGCT TGAAGAATTT GGGGTGGAAG 
GCTCAGATGC TGAAAATGTT TGTTACTTAC GAGATCTAGC TGATGCAAAT 
AGGCTTGCCA CTGTGATTCA ATCAAGCTCC AACGGGAATG CGGTTGTTAT 
CGGCGGTGGC TATATCGGTA TGGAGTGTGC TGCATCTTTA GTGATCAACA 
AAATCAATGT GACGATGGTT TTCCCAGAGG CTCACTGCAT GGCACGTCTC 
TTTACGCCCA AGATTGCAAG TTTGTATGAA GACTACTACA GGGCTAAAGG 
CGTAAAATTT ATAAAAGGAA CGGTTTTGAC ATCATTTGAG TTTGACTCGA 
ACAAAAAGGT TACTGCAGTT AATCTCAAAG ACGGGAGCCA CTTACCAGCC 
GACTTGGTTG TGGTTGGTAT CGGGATACGA CCAAACACAA GCTTGTTTGA 
AGGACAGTTA ACAATAGAGA AAGGAGGGAT AAAAGTGAAC AGCAGAATGC 
AGTCAAGTGA CAGCTCAGTG TATGCAATAG GCGATGTAGC TACATTTCCA 
GTGAAACTAT TTGGCGAAAT GAGGAGGCTT GAGCACGTGG ACTCAGCTAG 
GAAATCTGCG CGACACGCAG TTTCAGCCAT CATGGATCCA ATAAAGACCG 
GAGACTTTGA TTACTTACCA TTTTTCTACT CAAGGGTCTT CGCATTCTCA 
TGGCAATTCT ATGGAGACCC TACAGGTGAT GTTGTGCACT TTGGAGAGTA 
TGAAGACGGG AAATCATTTG GAGCGTATTG GGTTAAAAAG GGTCACCTTG 
TTGGATCTTT TCTTGAAGGA GGCACTAAAG AAGAATATGA AACTATCTCA 
AAGGCGACAC AATTGAAACC AGCTGTGACT ATTGATTTGG AAGAATTGGA 
AAGAGAAGGA CTCGGGTTTG CTCACACGGT TGTGAGTCAG CAGAAAGTTC 
CTGAAGTTAA GGATATTCCG AGCGCTGAGA TGGTGAAGCA ATCTGCAAGT 
GTGGTCATGA TAAAGAAGCC TTTGTATGTA TGGCACGCAG CTACTGGAGT 
CGTTGTGGCT GCATCTGTGG CTGCGTTCGC GTTTTGGTAT GGGAGGAGAC 
GCCGTAGATG GTGAGGAAAA AAAAAACTCT TCAAGTGTTG GATTCATTGG 
AAATGGAACT TGCTGTTGTT TGTAGCATTG GTGGGAGAAA TATAGAAGGG 
AGAGTCCAAA CTACTCAATG TGATTACATT GGTATACGAT TATTATATGC 
TTCTTTCAAA AAAAAAAAAA AAA

Gene models with high sequence identity

  1. AT3G27820.1 [+] show detail - monodehydroascorbate reductase 4
    E-value: 0; Score: 99.94

 

InterPro Scan Digest

Program Description E-value
HMMPanther MONODEHYDROASCORBATE REDUCTASE (NADH) 5.5e-167
HMMPanther DISULFIDE OXIDOREDUCTASE 5.5e-167
superfamily FAD/NAD(P)-binding domain 5.8e-42
superfamily FAD/NAD(P)-binding domain 5.7e-20
superfamily FAD/NAD-linked reductase, dimerisation 0.00000000000014
Gene3D no description 6.5e-21
Gene3D no description 1.2e-38
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
FPrintScan PNDRDTASEI 0.000000000000088
FPrintScan PNDRDTASEI 0.000000000000088
FPrintScan PNDRDTASEI 0.000000000000088
FPrintScan PNDRDTASEI 0.000000000000088
HMMPfam FAD-dependent pyridine nucleotide-disulphide oxidoreductase 5.4e-38
HMMPfam Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region 0.0000000000000042

See the detailed result >

AT3G27820.1

Model type
Protein coding
Short Description
monodehydroascorbate reductase 4
Curator Summary
Encodes a peroxisome membrane-bound monodehydroascorbate reductase, involved in the ascorbate-glutathione cycle which removes toxic H2O2
Computational Description
monodehydroascorbate reductase 4 (MDAR4); FUNCTIONS IN: monodehydroascorbate reductase (NADH) activity; INVOLVED IN: hydrogen peroxide catabolic process; LOCATED IN: peroxisomal membrane, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: FAD/NAD-linked reductase, dimerisation (InterPro:IPR016156), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (InterPro:IPR013027), Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (InterPro:IPR001327); BEST Arabidopsis thaliana protein match is: monodehydroascorbate reductase 1 (TAIR:AT3G52880.1); Has 27219 Blast hits to 27159 proteins in 2894 species: Archae - 579; Bacteria - 21951; Metazoa - 737; Fungi - 533; Plants - 600; Viruses - 0; Other Eukaryotes - 2819 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-10-E23 introduced

  • F19208 - stem::increased thickness / fruit::increased length

Mutant lines with disrupted or induced

2 mutant lines found