1. Home
  2. Full-length cDNAs
  3. RAFL05-12-I22

RAFL05-12-I22

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda01218)

full reading [+] show sequence

Nucleotide sequence: RAFL05-12-I22 - full reading 
ATGAACCAAA GTAAAAAACA AAGACTCGAG TTGATATGTC TAGTACTAAA 
GATATGTCAA CTGTCCAAAA CGCAACTCCT TTTAACGGCG TTGCCCCATC 
CACCACCGTG CGAGTTACAA TCGTCCAATC CTCCACCGTC TATAACGATA 
CTCCTGCCAC TATAGACAAA GCGGAGAAGT ATATTGTGGA GGCGGCAAGC 
AAGGGAGCAG AGCTAGTGTT GTTCCCGGAG GGGTTTATCG GTGGCTATCC 
TCGAGGTTTT AGGTTCGGTT TAGCGGTTGG CGTTCATAAC GAAGAAGGGC 
GTGATGAGTT TCGGAAGTAC CATGCTTCTG CTATTCATGT TCCTGGCCCT 
GAAGTAGCAA GATTGGCTGA CGTGGCTAGG AAAAACCATG TGTACTTGGT 
AATGGGAGCC ATAGAGAAGG AAGGGTATAC CCTCTATTGC ACAGTTCTTT 
TCTTTAGTCC ACAGGGTCAG TTCTTGGGCA AGCACCGTAA ACTCATGCCC 
ACAAGTTTGG AACGTTGCAT TTGGGGCCAA GGGGACGGAT CAACCATCCC 
CGTTTACGAC ACTCCCATTG GAAAACTCGG TGCTGCTATT TGCTGGGAGA 
ATAGGATGCC CCTCTACAGA ACTGCATTGT ACGCCAAAGG CATTGAGCTT 
TATTGTGCAC CTACTGCTGA TGGTTCGAAA GAATGGCAAT CGTCGATGCT 
TCACATTGCG ATCGAAGGTG GATGTTTCGT CTTGTCGGCT TGCCAATTCT 
GCCAGCGTAA ACATTTCCCT GATCATCCTG ACTACTTGTT TACCGATTGG 
TACGACGACA AAGAACATGA TTCTATTGTC TCCCAAGGTG GAAGTGTCAT 
TATTTCACCT TTGGGACAAG TTCTCGCCGG ACCAAACTTT GAATCAGAGG 
GTCTCGTCAC AGCTGATATT GATCTTGGTG ATATAGCAAG AGCCAAGTTA 
TACTTCGATT CGGTTGGACA TTACTCGAGA CCAGATGTTT TACACTTGAC 
CGTAAATGAG CACCCGAGGA AATCGGTTAC ATTCGTGACG AAGGTGGAGA 
AAGCTGAGGA TGACTCAAAC AAATAGTAAG AGACTTGAAG TTCGTATCTG 
CTGGAGTTAT GTCAATCGTA TGGAGTCAAG TCCAAAATGT TCTGTTGCGT 
TTTCATTTTA TGTTCAAGTT TATTTATCTT TCTCTTCAAT GGTAAGATCT 
ATGGAGTCAA GTAATAATGG TAAGACTTAT GTTGTTGAAT AAAACGAATT 
GGTTCTCT

Gene models with high sequence identity

  1. AT3G44310.1 [+] show detail - nitrilase 1
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
ProfileScan Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 45.072
PatternScan Nitrilase/cyanide hydratase, conserved site 0
PatternScan Nitrilase/cyanide hydratase, conserved site 0
HMMPfam Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 1.3e-41
HMMPanther NITRILASE-RELATED 3.6e-73
Gene3D Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 4.9e-71
superfamily Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 1.6e-77

See the detailed result >

AT3G44310.1

Model type
Protein coding
Short Description
nitrilase 1
Curator Summary
Mutants are resistant to indole-3-acetonitrile (IAN). NIT1 catalyzes the terminal activation step in indole-acetic acid biosynthesis. Predominantly expressed isoform of nitrilase isoenzyme family. Aggregation of NIT1 in cells directly abutting wound sites is one of the earliest events associated with wound and herbicide-induced cell death. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. It is also involved in the conversion of IAN to IAM (indole-3-acetamide) and other non-auxin-related metabolic processes.
Computational Description
nitrilase 1 (NIT1); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: apoplast, chloroplast, plasma membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 2 (TAIR:AT3G44300.1); Has 7572 Blast hits to 7496 proteins in 1731 species: Archae - 139; Bacteria - 4902; Metazoa - 443; Fungi - 562; Plants - 319; Viruses - 11; Other Eukaryotes - 1196 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-12-I22 introduced

  • F28245 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

4 mutant lines found