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RAFL05-14-B16

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87480)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL05-14-B16 - 5 prime EST
GTTGATCACT CGCTTTATAA ATCTCTCAGT TTCTTGCTCA CACCAACATC 
TCTCTAAGCT TCTTCTTCTA CCAATCTAAT TCCTCTCTTC AGCTTCTTGT
GTTGTGACGC ATACTCGTCG CAGTCTTGAG ATATGGCCGC CGCAGTTTCC
ACCGTCGGTG CCATCAACAG AGCTCCGTTG AGCTTGAACG GGTCAGGATC
AGGAGCTGTA TCAGCCCCAG CTTCAACCTT CTTGGGAAAG AAAGTTGTAA
CTGTGTCGAG ATTCGCACAG AGCAACAAGA AGAGCAACGG ATCATTCAAG
GTGTTGGCTG TGAAAGAAGA CAAACAAACC GATGGAGACA GATGGAGAGG
TCTTGCCTAC GACACTTCTG ATGATCAACA AGACATCACC AGAGGCAAGG
GTATGGTTGA CTCTGTCTTC CAAGCTCCTA TGGGAACCGG AACTCACCAC
GCTGTCCTTA GCTCATACGA ATACGTTAGC CAAGGCCTTA GGCAGTACAA
CTTGGACAAC ATGATGGATG GGTTTTACAT TGCTCCTGCT TTCATGGACA
AGCTTGTTGT TCACATCACC AAGAACTTCT TGACTCTGCC TAACATCAAG
GTTCCACTTA TTTTGGGTAT ATGGGGAGGC AAAGGTCAAG GTAAATCCTT
NCAGTGTGAG CTTG

Gene models with high sequence identity

  1. AT2G39730.3 [+] show detail - rubisco activase
    E-value: 0; Score: 99.85

3 prime EST [+] show sequence

Nucleotide sequence: RAFL05-14-B16 - 3 prime EST
AGGAATAAGA AGTGTAGTTT ATGACAAGGC AATTTTAAAT TACCAATGTG 
TTGTACATCA TAAAAAAAAA AAAAGGGAAC AAAAGAGGAC AAGATCCAAT
CCACAATTAC ACAAAGGAGA AAAATAAACC AAAAGAGACA ATAGGAGTAA
AACAGCAAAT TAATAAGCAG GATAATATTG CTCAAAAGTT GTAGACACAG
GTTCCATCGT CACTTCTAGC CGTTGGATCA AAGTTTTCAG CCACAGGATC
AGTACACCCT TCAGGAACTG GCAGGTTTAC TTGCTGGGCT CCTTTTCCTC
TGTTTTACCG TA

Gene models with high sequence identity

  1. AT2G39730.2 [+] show detail - rubisco activase
    E-value: 3e-176; Score: 100.00

AT2G39730.2

Model type
Protein coding
Short Description
rubisco activase
Curator Summary
Rubisco activase, a nuclear-encoded chloroplast protein that consists of two isoforms arising from alternative splicing in most plants. Required for the light activation of rubisco.
Computational Description
rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2331 Blast hits to 2283 proteins in 512 species: Archae - 328; Bacteria - 459; Metazoa - 248; Fungi - 427; Plants - 404; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G39730.3

Model type
Protein coding
Short Description
rubisco activase
Curator Summary
Rubisco activase, a nuclear-encoded chloroplast protein that consists of two isoforms arising from alternative splicing in most plants. Required for the light activation of rubisco.
Computational Description
rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2322 Blast hits to 2274 proteins in 510 species: Archae - 328; Bacteria - 451; Metazoa - 248; Fungi - 425; Plants - 405; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-14-B16 introduced

  • F04045 - Specific phenotypes were NOT observed.
  • F07525 - Specific phenotypes were NOT observed.
  • F12250 - Specific phenotypes were NOT observed.
  • F13315 - Specific phenotypes were NOT observed.
  • F16842 - Specific phenotypes were NOT observed.
  • F17719 - Specific phenotypes were NOT observed.
  • F19636 - Specific phenotypes were NOT observed.
  • F20423 - Specific phenotypes were NOT observed.
  • F20451 - Specific phenotypes were NOT observed.
  • F20942 - Specific phenotypes were NOT observed.
  • F26917 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

15 mutant lines found