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RAFL05-15-L01

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda07330)

full reading [+] show sequence

Nucleotide sequence: RAFL05-15-L01 - full reading 
ACTGGCCCAA GTCATTCAAC AACTTGGCTT TTATCTAATA AAGCCCCTCC 
CTCTCCGATA AACCTTAAAT AAAACCGGAG GGAGAGCAAA AAATAAAAAC 
AAAAATATAC AAAAACAAAA ACAAAAAATC ATAAAAAATG ATTGGAAAAG 
TTGTGGTCTC GGTGGCCTCC ATCCTCTTAA TAGTTGGAGT TGCCATAGGA 
GTCGTTGCCT ACATAAACAA AAATGGTGAT GCTAATCTGT CTCCCCAAAT 
GAAAGCGGTC CGAGGAATTT GCGAGGCGAC TTCCGACAAA GCCTCATGTG 
TCAAAACTCT CGAGCCGGTC AAGAGCGATG ACCCAAACAA GCTGATCAAG 
GCTTTCATGC TCGCTACCAG AGATGCTATA ACCCAATCAT CAAACTTCAC 
GGGTAAAACC GAAGGAAACT TGGGTTCGGG CATCTCGCCA AACAACAAAG 
CCGTTCTTGA TTACTGCAAG AAAGTTTTCA TGTACGCGCT TGAGGATCTC 
AGCACCATTG TTGAGGAAAT GGGTGAAGAT CTTAACCAGA TCGGGAGCAA 
AATCGACCAG CTTAAACAAT GGTTAACCGG TGTTTACAAT TACCAAACTG 
ATTGTCTTGA CGATATCGAA GAAGATGATT TAAGAAAGAC TATTGGAGAG 
GGCATTGCAA GCTCCAAGAT TCTCACTAGC AACGCTATTG ACATCTTCCA 
CACTGTCGTT AGCGCCATGG CCAAGCTTAA CTTGAAGGTC GAGGACTTCA 
AGAACATGAC AGGCGGAATC TTCGCTCCTT CAGACAAAGG AGCAGCTCCC 
GTCAACAAAG GAACCCCTCC TGTTGCTGAT GATTCTCCCG TGGCCGATCC 
AGATGGTCCT GCTCGCCGTC TTCTTGAAGA CATTGACGAG ACCGGAATCC 
CAACATGGGT TTCAGGTGCT GACAGGAAGC TCATGACTAA GGCTGGACGT 
GGCAGTAATG ACGGTGGTGC TAGGATCAGA GCAACCTTTG TGGTGGCTAA 
GGACGGAAGT GGACAGTTTA AGACTGTTCA ACAAGCCGTT AATGCTTGTC 
CTGAGAAAAA CCCAGGCCGT TGCATCATCC ACATCAAGGC TGGTATCTAC 
AGAGAGCAAG TGATCATCCC TAAGAAGAAG AACAACATCT TCATGTTCGG 
AGATGGTGCA AGAAAGACCG TCATTTCTTA CAACAGAAGT GTTAAACTCA 
GCCCTGGAAC CACCACTTCC CTTAGTGGCA CAGTCCAGGT TGAGTCCGAA 
GGATTCATGG CAAAATGGAT CGGATTCAAG AACACCGCCG GTCCAATGGG 
ACACCAAGCT GTGGCTATCA GAGTGAACGG AGACCGTGCC GTGATCTTCA 
ACTGCAGGTT CGACGGTTAC CAAGACACCT TGTACGTCAA CAATGGTCGT 
CAGTTCTACA GAAACATTGT CGTGTCAGGA ACAGTCGACT TCATCTTCGG 
CAAATCCGCA ACCGTGATCC AAAACTCACT CATTGTTGTC CGTAAAGGAA 
ACAAGGGACA ATTCAACACG GTCACAGCCG ATGGAAACGA AAAGGGTCTA 
GCGATGAAAA TCGGTATCGT CCTCCAAAAC TGTCGCATCG TTCCCGACAA 
GAAACTAGCG GCAGAGAGAT TAATCGTGGA GTCATACTTG GGAAGGCCAT 
GGAAGAAATT CTCTACCACC GTGATTATCA ACTCCGAGAT AGGAGATGTG 
ATTAGACCAG AAGGTTGGAA GATTTGGGAT GGGGAAAGTT TCCACAAGTC 
ATGTAGGTAC GTTGAGTACA ACAACCGTGG ACCAGGAGCT ATCACTAACC 
GAAGAGTTAA CTGGGTTAAG ATCGCCAGGT CTGCAGCTGA GGTCAATGAC 
TTCACTGTTG CTAACTGGTT AGGTCCTATT AACTGGATTC AAGAAGCCAA 
TGTTCCCGTC ACGCTTGGAT TATAAGGCGT ATAAATCAAA TCAAATCAAA 
CC

Gene models with high sequence identity

  1. AT2G47040.1 [+] show detail - Plant invertase/pectin methylesterase inhibitor superfamily
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Pectinesterase, catalytic 1.5e-111
HMMPfam Pectinesterase inhibitor 7.2e-20
HMMSmart Pectinesterase inhibitor 1.7e-32
HMMTigr Pectinesterase inhibitor 3.1e-05
superfamily Pectin lyase fold/virulence factor 2.3e-97
superfamily Pectinesterase inhibitor 1.4e-16
PatternScan Pectinesterase, active site 0
PatternScan Pectinesterase, active site 0
Gene3D Pectinesterase inhibitor 1.7e-05
Gene3D Pectin lyase fold 6.6e-110

See the detailed result >

AT2G47040.1

Model type
Protein coding
Short Description
Plant invertase/pectin methylesterase inhibitor superfamily
Curator Summary
Share high homologies with a group of pectin methylesterases (PME), pollen specific, and is required for enhancing the growth of pollen tube in style and transmitting tract tissues.
Computational Description
VANGUARD1 (VGD1); FUNCTIONS IN: enzyme inhibitor activity, pectinesterase activity; INVOLVED IN: pollen tube growth; LOCATED IN: endomembrane system, cell wall, plant-type cell wall; EXPRESSED IN: male gametophyte, flower, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Pectinesterase, active site (InterPro:IPR018040), Pectin lyase fold/virulence factor (InterPro:IPR011050), Pectinesterase, catalytic (InterPro:IPR000070), Pectinesterase inhibitor (InterPro:IPR006501), Pectin lyase fold (InterPro:IPR012334); BEST Arabidopsis thaliana protein match is: Plant invertase/pectin methylesterase inhibitor superfamily (TAIR:AT2G47030.1); Has 2977 Blast hits to 2908 proteins in 464 species: Archae - 6; Bacteria - 891; Metazoa - 1; Fungi - 197; Plants - 1856; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-15-L01 introduced

  • F01521 - Specific phenotypes were NOT observed.
  • F02428 - Specific phenotypes were NOT observed.
  • F07931 - Specific phenotypes were NOT observed.
  • F14915 - seed::increased size / seed::increased length
  • F19343 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

8 mutant lines found