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RAFL05-17-L23

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87513)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL05-17-L23 - 5 prime EST 
AAAATCCTCA GCCGCATCTC ACTTCTCATT CTCTAGAAAA AATGGCTTCG 
TCTTGTCTCT TCAATGCCTC TGTATCGTCC TTAAACCCTA AACAAGACCC 
CATCCGCCGC CACCGGTCAA CCTCTCTCCT CCGCCACCGC CCCGTCGTCA 
TCTCCTGTAC CGCCGATGGC AACAACATCA AAGCCCCGAT CGAGACAGCG 
GTCAAGCCTC CTCACCGTAC CGAGGATAAC ATTCGAGATG AGGCTCGTCG 
TAATCGTTCC AACGCCGTGA ATCCATTTTC AGCTAAATAC GTTCCGTTTA 
ATGCAGCTCC TGGATCCACG GAGTCTTACT CTNTTGACGA GATCGNGTAC 
CGTAGCCGCT CCGGTGGTTT GCNTGATGTC

Gene models with high sequence identity

  1. AT4G29840.1 [+] show detail - Pyridoxal-5'-phosphate-dependent enzyme family protein
    E-value: 0; Score: 98.95

3 prime EST [+] show sequence

Nucleotide sequence: RAFL05-17-L23 - 3 prime EST 
GATTCAAACT TTGCGATAGA ACTTTATTGG AAAACTGAGA AACAGATAAA 
GATCACAGAG AGATGAGTTG AAGAGTAACG CAAACAAACG TCTAAAAGTT 
TTCAAAAAAC AAAACAGCTA AAGATTATGG TCTTGTCTCT TATGACGTAA 
GTGTATTACT TCCTAAGTAA CTCTTGAGAA CATCCATGAC AGCTCCGAAA 
TCTGCTTTCA CATCAACAGG AGGATTGGAG AATCTGCAAG CCATGTCAGG 
GATGGCATTG GAGTGATAAT CTATCTTAGA CTGAGTAAAC TTCAACCCAT 
GAGCAGTACT CACTACCATA GTTCGATCAG TCGGTGCAAT CACTCCTTGA 
TTCCTCAGCT TGAACAGAGC AGTTAGAGCA ACACCTGTAT GAGGACAGAT 
AAACATTCCT GTCGAATCCG CTTGAGCCAT CGCATCCATC AGCTCCTCCT 
CTGTGGCTTC TTCTACAATA CCATTGCACT TCTTGAGAGC GTACACAGCT 
CTATCGATGG AGACAGGGTC ACCGATCTGA ATCGCA

Gene models with high sequence identity

  1. AT4G29840.1 [+] show detail - Pyridoxal-5'-phosphate-dependent enzyme family protein
    E-value: 0; Score: 99.81

AT4G29840.1

Model type
Protein coding
Short Description
Pyridoxal-5'-phosphate-dependent enzyme family protein
Curator Summary
threonine synthase
Computational Description
METHIONINE OVER-ACCUMULATOR 2 (MTO2); FUNCTIONS IN: threonine synthase activity; INVOLVED IN: threonine biosynthetic process, metabolic process, cellular amino acid metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Threonine synthase (InterPro:IPR004450), Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Serine/threonine dehydratase, pyridoxal-phosphate-binding site (InterPro:IPR000634); BEST Arabidopsis thaliana protein match is: Pyridoxal-5'-phosphate-dependent enzyme family protein (TAIR:AT1G72810.1); Has 6824 Blast hits to 6824 proteins in 2025 species: Archae - 409; Bacteria - 4266; Metazoa - 101; Fungi - 24; Plants - 83; Viruses - 0; Other Eukaryotes - 1941 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-17-L23 introduced

  • F00336 - Specific phenotypes were NOT observed.
  • F11149 - Specific phenotypes were NOT observed.
  • F17146 - seed::increased size / seed::increased length / seed::decreased width
  • F26030 - seed::increased size / seed::increased length

Mutant lines with disrupted or induced

6 mutant lines found