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  3. RAFL05-18-H15

RAFL05-18-H15

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda17124)

full reading [+] show sequence

Nucleotide sequence: RAFL05-18-H15 - full reading 
CCGGTTAACA CCTAGGTTCT TGTTTCCTAA CAAAGGCTCT ACTTTTTCTT 
TAGTTAACTA CTATGGCTCA TTTCCTCGAG ACACAGGAGC CCCTCGTATT 
CTCCGGGAAG AAACGAAACG ATCGCGATGA CGAAGACGGT GACGCCTTGG 
TTGCGAAGAA ATCAGCACTG GCGGTGTGTG ATGCCGACCC TGCAGCGGCC 
ATAGCGAATA TCCGACATGA GTTTGGGGAG CACGGGGGAG TAAACATGTC 
CATCGAAGCC TCCGCCACCT TCACCGTCAT GGAACCGGAT ACCATGAGGC 
GGATGTTCAC CGGAGAACTG GGACCGGACA ACGACTTCTA CGTATACAGC 
CGACACTTCA ACCCCACCGT GCTCAACCTC AGCCGCCAGA TGGCTGCTCT 
CGAAGGCACC CAAGCTGCCT ACTGTACCTC TAGCGGTATG TCGGCGATAT 
CGTCAGTGAT GCTGCAACTG TGCAGCAGCG GAGGACACGT GGTTGCGGCA 
AGCACGCTAT ACGGAGGAAC ACACGCTTTG CTCTCTCATT TCTTGCCACG 
GACATGTAAC ATAACCACCT CCTTCGTTGA CATAACGGAC CATGGCGCCG 
TGGCAAACGC GATCGTTGAG GGTAGGACAC AGGTTCTCTA CTTTGAGTCG 
GTGGCAAACC CGACGCTGAC TGTGGCTGAC ATACCTGAGC TGAGCCGTAT 
GGCACACGAG AAGGGCGTGA CGGTGGTGGT GGACAACACA TTCGCCCCCA 
TGGTGCTGTC TCCGGCCAAG CTTGGAGCAG ATGTGGTGGT TCACAGTATC 
TCCAAGTTCA TCAGTGGTGG GGCTGACATC ATCGCAGGGG CCGTGTGTGG 
GAGTGAGAAT TTGGTGAAAG AGATGATGGA TCTTCGTGGC GGATCTCTGA 
TGCTTCTAGG TCCCACCATG AACGCCAAGG TGGCTTTCGA GCTCTCCGAG 
CGAATCCCTC ACTTGGGCCT ACGCATGAGA GAGCACAGCC ACAGAGCCCA 
AGTGTATGCG GAGAGAATGA GGGATTTGGG CATGAAAGTC ATATATCCAG 
GGCTCGAGAC CCACCCGCAG CACAAGCTAT TCAAAGGGAT GGTGAATAGA 
GACTATGGAT ACGGAGGATT GCTGTCGATA GACATGGAGA CAGAGGAGAA 
AGCCAACAAG CTCATGGCAT ATCTCCAGAA CGCCACTCAG TTTGGCTTCA 
TGGCCGTCAG TTTGGGTTAC TACGAGACAC TCATGTCTTG CTCCGGGAGC 
AGCACTAGCA GCGAGCTTGA CCCCTCACAG AAGGAAGCTG CAGGCATCTC 
TCCTGGTCTT GTACGAATGT CGGTGGGGTA TGTGGGTACA TTGGAGCAGA 
AGTGGACACA ATTCGAGAAA GCATTCCTCA GAATGTAAAA TGTCTTCTTC 
TATATATGAT GGTTTTCTTT CCCCCAAATA CCAACAATTC ATCATCTGCG 
TTATATGTAT GATGTGGCCA ATGTTCAGTC TCTTATTTGT TATATCACAA 
TGTTCTAGTC TTCGGTAAAA AAAAAA

Gene models with high sequence identity

  1. AT1G64660.1 [+] show detail - methionine gamma-lyase
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPIR Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 5.7e-118
HMMPanther METHIONINE GAMMA-LYASE 5.6e-221
HMMPanther Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 5.6e-221
Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 3e-57
Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 2 8.6e-19
HMMPfam Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 8.2e-88
superfamily Pyridoxal phosphate-dependent transferase, major domain 1.2e-80

See the detailed result >

AT1G64660.1

Model type
Protein coding
Short Description
methionine gamma-lyase
Curator Summary
Encodes a functional methionine gamma-lyase, a cytosolic enzyme catalyzes the degradation of methionine into methanethiol, alpha-ketobutyrate and ammonia. The catabolism of excess methionine is important to methionine homeostasis.
Computational Description
methionine gamma-lyase (MGL); CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cys/Met metabolism, pyridoxal phosphate-dependent enzyme (InterPro:IPR000277), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (InterPro:IPR015422); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT3G01120.1); Has 19547 Blast hits to 19544 proteins in 2474 species: Archae - 216; Bacteria - 11847; Metazoa - 211; Fungi - 771; Plants - 270; Viruses - 0; Other Eukaryotes - 6232 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-18-H15 introduced

  • F05430 - seed::increased size / seed::increased length
  • F16836 - rosette leaf::decreased size / rosette leaf::increased width / rosette leaf::abnormal / whole plant::decreased height / sporophyte vegetative stage::decreased speed
  • F20647 - Specific phenotypes were NOT observed.
  • F25006 - Specific phenotypes were NOT observed.
  • F26411 - Specific phenotypes were NOT observed.
  • F27113 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

6 mutant lines found