1. Home
  2. Full-length cDNAs
  3. RAFL05-18-L20

RAFL05-18-L20

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda17126)

full reading [+] show sequence

Nucleotide sequence: RAFL05-18-L20 - full reading 
CCGAAGTGAA TTCGATTTAC ACTTTTTTGT CCTTTTTTAT TAATAAATCA 
CTGACCCGAA AATAAAAATA GAAGCAAAAC TTCATGTCGT GGTTTCTAAT 
AGCGGTGGCG ACAATCGCCG CCGTCGTATC CTACAAGCTA ATCCAACGGC 
TAAGATACAA GTTCCCACCA GGCCCAAGCC CCAAGCCGAT CGTCGGTAAC 
CTCTACGACA TAAAACCGGT CCGGTTCAGA TGTTACTACG AGTGGGCTCA 
ATCTTATGGA CCAATCATAT CGGTCTGGAT CGGTTCAATT CTAAACGTGG 
TTGTATCTAG CGCCGAGCTA GCAAAAGAAG TTCTGAAAGA ACACGACCAG 
AAACTCGCCG ACCGGCACCG GAACAGATCG ACGGAAGCAT TTAGCCGCAA 
CGGTCAGGAT CTTATATGGG CCGATTATGG GCCTCATTAC GTGAAGGTGA 
GAAAAGTTTG CACGCTTGAG CTCTTCACAC CGAAACGACT CGAGTCTCTC 
AGACCTATCC GTGAAGATGA AGTCACCGCC ATGGTTGAAT CCGTCTTCAG 
AGACTGTAAC CTTCCTGAAA ACAGAGCAAA AGGTTTACAA CTGAGGAAGT 
ACTTAGGAGC GGTTGCGTTC AACAACATAA CGCGGCTAGC CTTTGGGAAG 
CGTTTTATGA ACGCTGAAGG TGTTGTGGAC GAGCAAGGGC TTGAGTTCAA 
GGCCATAGTA TCCAACGGTC TGAAGCTAGG TGCTTCACTG TCAATAGCTG 
AACACATCCC GTGGCTCAGG TGGATGTTTC CGGCTGATGA GAAGGCGTTT 
GCTGAGCACG GGGCTCGTCG TGACCGCCTC ACTCGAGCTA TCATGGAGGA 
GCATACTTTG GCCCGTCAAA AGTCTAGTGG AGCGAAACAG CATTTCGTTG 
ATGCGTTGCT AACGTTGAAG GATCAGTATG ATCTTAGTGA GGATACTATC 
ATTGGTCTTC TATGGGATAT GATCACGGCA GGGATGGACA CGACAGCGAT 
AACAGCGGAA TGGGCGATGG CGGAAATGAT CAAGAATCCA AGAGTGCAAC 
AAAAAGTGCA AGAAGAGTTC GACAGAGTGG TTGGACTTGA CCGGATCTTA 
ACCGAGGCAG ATTTCTCCCG CTTACCTTAC TTGCAATGCG TGGTGAAAGA 
GTCATTCAGG CTGCATCCTC CAACGCCTCT AATGCTACCT CACCGAAGCA 
ACGCAGATGT CAAGATCGGA GGCTATGATA TTCCCAAAGG ATCAAACGTT 
CATGTGAATG TGTGGGCTGT GGCTAGAGAC CCGGCTGTAT GGAAAAATCC 
ATTTGAGTTT AGACCAGAGA GATTCTTGGA AGAAGATGTT GACATGAAGG 
GTCATGATTT TAGGCTGCTT CCGTTTGGAG CTGGAAGACG GGTTTGTCCC 
GGTGCACAAC TTGGTATCAA TTTGGTAACT TCGATGATGA GTCATTTGCT 
TCACCATTTT GTTTGGACAC CTCCTCAAGT GACTAAACCG GAGGAGATTG 
ACATGTCTGA AAACCCTGGA CTCGTTACTT ACATGCGTAC CCCTGTGCAA 
GCGGTTGCAA CGCCTCGGTT GCCTTCGGAT CTGTACAAAC GCGTGCCTTA 
CGATATGTAA ATGTCACTCT GATCTACCTT TTGTTGCTGC TGCTCATGCT 
CTTGTTGTTG TTTGTAGACA TGTTTCTTGT GGTTTATCAT CGAAAAACTT 
TTGATGAGAT TTGTTGTATC AATTCTCTCA AGGAGAAGTA AAGAAGCCAT 
CTTCTTTGTC GAAAAAAAAA A

Gene models with high sequence identity

  1. AT2G40890.1 [+] show detail - cytochrome P450, family 98, subfamily A, polypeptide 3
    E-value: 0; Score: 99.77

 

InterPro Scan Digest

Program Description E-value
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, E-class, group I 1.1e-49
FPrintScan Cytochrome P450, C-terminal 0.0000000014
FPrintScan Cytochrome P450, C-terminal 0.0000000014
FPrintScan Cytochrome P450, C-terminal 0.0000000014
HMMPfam Cytochrome P450 2.4e-102
superfamily Cytochrome P450 3e-128
HMMPanther CYTOCHROME P450, SUBFAMILY 98A 0
HMMPanther Cytochrome P450 0
PatternScan Cytochrome P450, conserved site 0
Gene3D Cytochrome P450 1e-120

See the detailed result >

AT2G40890.1

Model type
Protein coding
Short Description
cytochrome P450, family 98, subfamily A, polypeptide 3
Curator Summary
encodes coumarate 3-hydroxylase (C3H), a P450-dependent monooxygenase. Involved in lignin biosynthesis and flavonoid biosynthesis. Also affects the biosynthesis of coumarins such as scopoletin and scopolin as a branching-out-pathway from the phenylpropanoid acid level.
Computational Description
cytochrome P450, family 98, subfamily A, polypeptide 3 (CYP98A3); FUNCTIONS IN: monooxygenase activity, p-coumarate 3-hydroxylase activity; INVOLVED IN: coumarin biosynthetic process, lignin biosynthetic process, phenylpropanoid biosynthetic process, flavonoid biosynthetic process; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, microsome; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 98, subfamily A, polypeptide 9 (TAIR:AT1G74550.1); Has 33899 Blast hits to 33682 proteins in 1752 species: Archae - 48; Bacteria - 3997; Metazoa - 11870; Fungi - 7164; Plants - 9566; Viruses - 3; Other Eukaryotes - 1251 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-18-L20 introduced

  • F24930 - Specific phenotypes were NOT observed.
  • F25937 - seed::increased size / seed::increased length
  • F26227 - rosette leaf::dark green / rosette leaf::increased width / stem::abnormal / cauline leaf::dark green / cauline leaf::dark green / whole plant::decreased height

Mutant lines with disrupted or induced

4 mutant lines found