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  3. RAFL05-20-O23

RAFL05-20-O23

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda07458)

full reading [+] show sequence

Nucleotide sequence: RAFL05-20-O23 - full reading 
ACCTTTCCCT CACCTGATAT TTATTTTCTT ACCTTAAATA CGACGGTGCT 
TCACTGAGTC CGACTCAGTT AACTCGTTCC TCTCTCTGTG TGTGGTTTTG 
GTAGACGACG ACGACGATAA TGGAGGAGCT AGATCGTTCA CGTACTTTTG 
CCAGAGACGT CAAACGTATC GTCGTTAAGG TTGGGACAGC AGTTGTTACT 
GGAAAAGGTG GAAGATTGGC TCTTGGTCGT TTAGGAGCAC TGTGTGAACA 
GCTTGCGGAA TTAAACTCGG ATGGATTTGA GGTGATATTG GTGTCATCTG 
GTGCGGTTGG TCTTGGCAGG CAAAGGCTTC GTTATCGACA ATTAGTCAAT 
AGCAGCTTTG CGGATCTTCA GAAGCCTCAG ACTGAACTTG ATGGGAAGGC 
TTGTGCTGGT GTTGGACAAA GCAGTCTTAT GGCTTACTAT GAGACTATGT 
TTGACCAGCT TGATGTGACG GCAGCTCAAC TTCTGGTGAA TGACAGTAGT 
TTTAGAGACA AGGATTTCAG GAAGCAACTT AATGAAACTG TCAAGTCTAT 
GCTTGATTTG AGGGTTATTC CAATTTTCAA TGAGAATGAT GCTATTAGCA 
CCCGAAGAGC CCCATATCAG GATTCTTCTG GTATTTTCTG GGATAACGAT 
AGCTTAGCTG CTCTACTGGC GTTGGAACTG AAAGCTGATC TTCTGATTCT 
TCTGAGCGAT GTTGAAGGTC TTTACACAGG CCCTCCAAGT GATCCTAACT 
CAAAGTTGAT CCACACTTTT GTTAAAGAAA AACATCAAGA TGAGATTACA 
TTCGGCGACA AATCAAGATT AGGGAGAGGG GGTATGACTG CAAAAGTCAA 
AGCTGCAGTC AATGCAGCTT ATGCTGGGAT TCCTGTCATC ATAACCAGTG 
GGTATTCAGC TGAGAACATA GATAAAGTCC TCAGAGGACT ACGTGTTGGA 
ACCTTGTTTC ATCAAGATGC TCGTTTATGG GCTCCGATCA CAGATTCTAA 
TGCTCGTGAC ATGGCAGTTG CTGCGAGGGA AAGTTCCAGA AAGCTTCAGG 
CCTTATCTTC GGAAGACAGG AAAAAAATTC TGCTTGATAT TGCCGATGCC 
CTTGAAGCAA ATGTTACTAC AATCAAAGCT GAGAATGAGT TAGATGTAGC 
TTCTGCACAA GAGGCTGGGT TGGAAGAGTC AATGGTGGCT CGCTTAGTTA 
TGACACCTGG AAAGATCTCG AGCCTTGCAG CTTCAGTTCG TAAGCTAGCT 
GATATGGAAG ATCCAATCGG CCGTGTTTTA AAGAAAACAG AGGTGGCAGA 
TGGTCTTGTC TTAGAGAAGA CCTCATCACC ATTAGGCGTA CTTCTGATTG 
TTTTTGAATC CCGACCTGAT GCACTTGTAC AGATAGCTTC ACTTGCCATC 
CGTAGTGGAA ATGGTCTTCT GCTGAAGGGT GGAAAGGAGG CCCGGCGATC 
AAATGCTATC TTACACAAGG TGATCACTGA TGCAATTCCA GAGACTGTTG 
GGGGTAAACT CATTGGACTT GTGACTTCAA GAGAAGAGAT TCCTGATTTG 
CTTAAGCTTG ATGACGTTAT CGATCTTGTG ATCCCAAGAG GAAGCAACAA 
GCTTGTTACT CAGATAAAAA ATACTACAAA AATCCCTGTG CTAGGTCATG 
CTGATGGAAT CTGTCATGTA TATGTCGACA AGGCTTGTGA TACGGATATG 
GCAAAGCGCA TAGTTTCTGA TGCAAAGTTG GACTATCCAG CAGCCTGTAA 
TGCGATGGAA ACCCTTCTTG TGCATAAGGA TCTAGAGCAG AATGCTGTGC 
TTAATGAGCT TATTTTTGCT CTGCAGAGCA ATGGAGTCAC TTTGTATGGT 
GGACCAAGGG CAAGTAAGAT ACTGAACATA CCAGAAGCAC GGTCATTCAA 
CCATGAGTAC TGTGCCAAGG CTTGCACTGT TGAAGTTGTA GAAGACGTTT 
ATGGTGCTAT AGATCACATT CACCGACATG GGAGTGCACA CACAGACTGC 
ATTGTGACAG AGGATCACGA AGTTGCAGAG CTATTCCTTC GCCAAGTGGA 
TAGCGCTGCT GTGTTCCACA ACGCCAGCAC AAGATTCTCA GATGGTTTCC 
GATTTGGACT TGGTGCAGAG GTGGGGGTAA GCACGGGCAG GATCCATGCT 
CGTGGTCCAG TCGGGGTCGA AGGATTACTT ACAACGAGAT GGATAATGAG 
AGGAAAAGGA CAAGTTGTCG ACGGAGACAA TGGAATTGTT TACACCCATC 
AGGACATTCC CATCCAAGCT TAAACAAGAC TTCCGAGTGT GTGTTTGTGT 
ATTTGGTTGA GACTTGAGGA GAGACACAGA GGAGGATGGG CTTTTTTGTT 
TCCTCTCTGC TTAGTACTCA TATCCTATCA TTATTATTAT TACTACTACT 
TATTATTGAA ACCCTCGCTT ATGTAGTGGT TTTGATTTAG GGTTAGGATT 
GCACCAAAAA TAAGATCCAC TTTACCACTT AGTCTTGCTC ATAAGTACGA 
TGAAGAACAT TTAATTAGCT TCTCTTCTTG TCATTGTAAG CTACCTACAC 
ATTTCTGATC TTTATCAAGA TACTACTACT TTTCAAAAAA AAAAAAAAAA

Gene models with high sequence identity

  1. AT2G39800.1 [+] show detail - delta1-pyrroline-5-carboxylate synthase 1
    E-value: 0; Score: 99.96

 

InterPro Scan Digest

Program Description E-value
PatternScan Glutamate 5-kinase, conserved site 0
PatternScan Gamma-glutamyl phosphate reductase GPR, conserved site 0
HMMPanther DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE 0
HMMPanther GLUTAMATE SEMIALDEHYDE DEHYDROGENASE 0
HMMPfam Aspartate/glutamate/uridylate kinase 3.9e-37
HMMPfam Aldehyde dehydrogenase 6.9e-08
HMMTigr Delta l-pyrroline-5-carboxylate synthetase 0
HMMTigr Glutamate 5-kinase, ProB-related 7e-26
HMMTigr Gamma-glutamyl phosphate reductase GPR 8.9e-161
Gene3D Aspartate/glutamate/uridylate kinase 9.9e-18
Gene3D Aldehyde dehydrogenase, N-terminal 5.9e-56
Gene3D Aldehyde dehydrogenase, C-terminal 3.6e-64
superfamily Aldehyde/histidinol dehydrogenase 4.9e-99
superfamily Aspartate/glutamate/uridylate kinase 3.7e-53
HMMPIR Delta l-pyrroline-5-carboxylate synthetase 0
FPrintScan Glutamate 5-kinase 3.4e-56
FPrintScan Glutamate 5-kinase 3.4e-56
FPrintScan Glutamate 5-kinase 3.4e-56
FPrintScan Glutamate 5-kinase 3.4e-56
FPrintScan Glutamate 5-kinase 3.4e-56

See the detailed result >

AT2G39800.1

Model type
Protein coding
Short Description
delta1-pyrroline-5-carboxylate synthase 1
Curator Summary
encodes a delta1-pyrroline-5-carboxylate synthase that catalyzes the rate-limiting enzyme in the biosynthesis of proline. Gene is expressed in reproductive organs and tissues under non-stress conditions but in the whole plant under water-limiting condition. Expression is also induced by abscisic acid and salt stress in a light-dependent manner. P5CS1 appears to be involved in salt stress responses related to proline accumulation, including protection from reactive oxidative species. P5CS1 appears to be present in different cells and/or different subcellular locations from P5CS2 in a tissue-dependent manner.
Computational Description
delta1-pyrroline-5-carboxylate synthase 1 (P5CS1); FUNCTIONS IN: delta1-pyrroline-5-carboxylate synthetase activity; INVOLVED IN: in 9 processes; LOCATED IN: chloroplast, membrane, cytoplasm; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: C globular stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Glutamate 5-kinase (InterPro:IPR001057), Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Glutamate 5-kinase, conserved site (InterPro:IPR019797), Aldehyde dehydrogenase, N-terminal (InterPro:IPR016162), Gamma-glutamyl phosphate reductase GPR, conserved site (InterPro:IPR020593), Aldehyde dehydrogenase, C-terminal (InterPro:IPR016163), Aldehyde/histidinol dehydrogenase (InterPro:IPR016161), Delta l-pyrroline-5-carboxylate synthetase (InterPro:IPR005766), Gamma-glutamyl phosphate reductase GPR (InterPro:IPR000965), Aldehyde dehydrogenase (InterPro:IPR015590), Glutamate 5-kinase, ProB-related (InterPro:IPR005715); BEST Arabidopsis thaliana protein match is: delta 1-pyrroline-5-carboxylate synthase 2 (TAIR:AT3G55610.1); Has 14932 Blast hits to 14924 proteins in 2380 species: Archae - 163; Bacteria - 9254; Metazoa - 175; Fungi - 266; Plants - 182; Viruses - 0; Other Eukaryotes - 4892 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-20-O23 introduced

  • F05708 - rosette leaf::decreased size / cauline leaf::epinastic / sporophyte vegetative stage::decreased speed / seed::present in fewer numbers in organism / fruit::decreased length / stem::present in greater numbers in organism / root::present in greater numbers in organism
  • F25546 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

3 mutant lines found