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RAFL05-20-P15

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda01337)

full reading [+] show sequence

Nucleotide sequence: RAFL05-20-P15 - full reading 
ACAACCGATT TTCTCCATTT TCTTCAAAAC GATTCGGGTC AAGGTTATTG 
ACTTTCTCAT TCAGTGAAGC TTGAATCATG GCCTCGAGAA TTGCTAAAGA 
TGTGACTGAA TTGATTGGGA ACACTCCATT GGTGTTGAAC AATGTTGCTG 
AAGGATGTGT TGGTCGTGTT GCTGCTAAGC TTGAGATGAT GGAACCGTGC 
TCTAGCGTCA AAGACAGGAT TGGTTTTAGC ATGATTTCTG ATGCAGAAAA 
GAAGGGTCTT ATCAAACCAG GAGAGAGTGT GCTGATTGAG CCAACAAGTG 
GAAACACTGG AGTTGGGTTA GCATTCACGG CAGCTGCCAA AGGCTACAAG 
CTTATTATTA CAATGCCAGC TTCTATGAGT ACTGAGAGAA GAATCATTCT 
CTTAGCTTTT GGAGTTGAGT TGGTTTTAAC TGACCCAGCT AAGGGCATGA 
AAGGAGCTAT CGCAAAGGCG GAAGAGATTT TGGCGAAAAC ACCCAATGGT 
TACATGCTTC AGCAGTTTGA GAACCCTGCC AACCCTAAGA TCCACTATGA 
GACTACGGGA CCTGAGATAT GGAAAGGCAC TGGTGGCAAA ATCGATGGCT 
TTGTTTCTGG GATTGGTACT GGTGGTACCA TTACAGGTGC TGGGAAGTAT 
CTTAAAGAAC AGAACGCAAA CGTCAAGCTG TATGGAGTGG AGCCAGTTGA 
AAGTGCTATT CTATCCGGTG GGAAGCCAGG TCCTCACAAG ATTCAAGGGA 
TAGGAGCTGG TTTTATACCA AGTGTATTGA ATGTTGATCT TATTGACGAA 
GTTGTTCAGG TTTCAAGTGA TGAATCCATT GACATGGCAA GGCAGCTTGC 
TCTTAAAGAA GGTCTTCTTG TGGGAATATC ATCCGGTGCA GCAGCTGCTG 
CAGCAATTAA ACTTGCACAG AGGCCAGAAA ACGCTGGGAA GCTATTTGTG 
GCGATATTCC CGAGTTTCGG GGAGAGGTAT CTATCAACGG TACTTTTCGA 
TGCGACAAGG AAAGAAGCGG AAGCCATGAC CTTCGAGGCT TGAACATTCT 
CCATTTCTTC TTAAGAGACG CCAAAATAAA AGAGATGTTC AGTTTCTCCT 
ATAGAGACTC TTCATCTTTA GTTACATTGC GTCTTTGCTT CCATCTGTAT 
CTTCTCTTGT GTGCCAATAA AAGTCAAACT AGATTTTCTC TGTTTCTTTT 
GTGAACCACT TGCTTGTTAA TGAAGTTTAA ATTTGCCAAA AAAAAAAAAA 
AAAA

Gene models with high sequence identity

  1. AT4G14880.1 [+] show detail - O-acetylserine (thiol) lyase (OAS-TL) isoform A1
    E-value: 0; Score: 99.76

 

InterPro Scan Digest

Program Description E-value
superfamily Pyridoxal phosphate-dependent enzyme, beta subunit 1.8e-91
HMMPanther CYSTEINE SYNTHASE 1.3e-145
HMMPanther SER/THR DEHYDRATASE, TRP SYNTHASE 1.3e-145
PatternScan Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site 0
Gene3D no description 2.6e-68
HMMTigr Cysteine synthase K/M 2.5e-214
HMMTigr Cysteine synthase A 1.1e-228
HMMPfam Pyridoxal phosphate-dependent enzyme, beta subunit 1.4e-54

See the detailed result >

AT4G14880.1

Model type
Protein coding
Short Description
O-acetylserine (thiol) lyase (OAS-TL) isoform A1
Curator Summary
Encodes a cytosolic isoform of cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis and for the fixation of inorganic sulfide. It catalyzes the formation of cysteine from O-acetylserine and inorganic sulfide. Gene expression is predominant in the root cortex and the xylem parenchyma. Gene expression is induced in leave, stems and roots by high salt and heavy metal stresses, mediated by ABA. Lines carrying semi-dominant mutations exhibit early senescence.
Computational Description
O-acetylserine (thiol) lyase (OAS-TL) isoform A1 (OASA1); FUNCTIONS IN: protein binding, cysteine synthase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site (InterPro:IPR001216), Cysteine synthase A (InterPro:IPR005859), Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Cysteine synthase K/M (InterPro:IPR005856); BEST Arabidopsis thaliana protein match is: O-acetylserine (thiol) lyase (OAS-TL) isoform A2 (TAIR:AT3G22460.1); Has 20496 Blast hits to 20479 proteins in 2663 species: Archae - 403; Bacteria - 14284; Metazoa - 350; Fungi - 659; Plants - 549; Viruses - 2; Other Eukaryotes - 4249 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL05-20-P15 introduced

  • F09720 - rosette leaf::decreased size / cauline leaf::abnormal / stem::present in greater numbers in organism / stem::abnormal
  • F17928 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

8 mutant lines found