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  3. RAFL06-10-A16

RAFL06-10-A16

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda09376)

full reading [+] show sequence

Nucleotide sequence: RAFL06-10-A16 - full reading 
GAGAAGAGGA GGAGAACACA AATCCAATTC ACACTGGAAA CTGTTGGGTC 
GGATCCTGAG TGATATTTCG AGACCGATGA TGTGGAAGAA CATTGCTGGG 
TTGTCGAAGG CAGCGGCAGC GGCAAGAACA CACGGATCTC GGCGGTGCTT 
TTCCACAGCG ATTCCTGGTC CTTGCATCGT CCACAAGCGT GGTGCTGATA 
TTCTTCACGA TCCATGGTTC AATAAGGACA CAGGTTTTCC TTTGACTGAG 
AGAGATAGAT TAGGGATACG AGGCTTGCTT CCTCCTCGTG TTATGACCTG 
TGTACAGCAA TGTGATCGTT TCATTGAGTC GTTTCGTTCG CTAGAGAACA 
ACACAAAAGG AGAGCCTGAA AACGTTGTTG CATTAGCTAA ATGGAGGATG 
TTGAATAGAT TGCATGACCG TAATGAGACT TTGTACTACC GAGTTCTTAT 
TGATAACATC AAAGATTTTG CCCCCATTAT ATACACTCCT ACGGTTGGAC 
TTGTTTGTCA GAACTATTCT GGTTTGTATA GAAGACCAAG AGGAATGTAC 
TTCAGCGCCA AAGACAAAGG AGAGATGATG TCTATGATTT ATAACTGGCC 
TGCTCCGCAG GTAGATATGA TTGTCATCAC TGATGGTAGC CGTATTTTGG 
GCTTAGGAGA CCTTGGAGTA CAGGGAATTG GGATTCCTAT TGGAAAGCTC 
GACATGTATG TCGCTGCTGC AGGAATCAAT CCACAGAGAG TTTTGCCAAT 
TATGTTGGAT GTAGGCACAA ATAATGAAAA GCTCCTACAG AATGATCTCT 
ATTTAGGAGT CAGACAACCT AGGTTGGAAG GAGAGGAGTA CCTTGAGATT 
ATTGATGAAT TTATGGAAGC TGCATTTACA CGTTGGCCAA AGGCCGTCGT 
ACAGTTCGAA GATTTCCAAG CGAAATGGGC TTTCGGAACA TTGGAAAGAT 
ATCGGAAGAA ATTTTGCATG TTCAATGATG ATGTCCAGGG AACTGCTGGT 
GTTGCACTGG CTGGACTATT GGGAACTGTA AGAGCTCAAG GTCGGCCGAT 
ATCCGACTTT GTGAACCAAA AGATTGTTGT GGTGGGAGCT GGAAGTGCTG 
GGCTTGGTGT AACTAAGATG GCAGTACAGG CAGTTGCCAG AATGGCAGGT 
ATCAGCGAGA GTGAAGCAAC AAAAAACTTT TACCTGATAG ATAAAGATGG 
TCTTGTCACC ACGGAGAGGA CAAAACTTGA CCCAGGAGCT GTACTTTTTG 
CCAAAAATCC AGCTGAGATA CGCGAGGGAG CAAGTATCGT TGAAGTGGTG 
AAGAAAGTGA GGCCACATGT TCTTCTTGGT TTATCTGGAG TTGGTGGTAT 
CTTTAATGAA GAAGTTCTCA AGGCGATGCG AGAATCTGAC TCATGCAAGC 
CTGCCATTTT TGCCATGTCG AATCCCACTT TGAATGCCGA GTGCACTGCT 
GCTGATGCTT TCAAACACGC CGGAGGAAAC ATTGTCTTTG CAAGTGGAAG 
TCCATTTGAG AACGTTGAAC TTGAGAATGG TAAAGTAGGA CATGTGAATC 
AGGCTAACAA CATGTATTTA TTCCCGGGGA TCGGGCTAGG GACTCTTCTC 
TCTGGTGCAC GTATCGTAAC TGATGGAATG CTACAAGCAG CTTCTGAATG 
CCTTGCGTCC TACATGACCG ATGAAGAAGT CCAAAAAGGC ATCCTTTACC 
CTTCAATCAA CAACATCCGA CACATAACAG CTGAAGTCGG GGCAGCTGTT 
CTACGAGCTG CGGTGACTGA TGACATTGCA GAAGGACATG GTGATGTTGG 
TCCCAAAGAT CTCAGCCACA TGTCTAAGGA GGATACAGTG AATTACATTA 
CACGCAACAT GTGGTTCCCT GTTTACAGCC CTCTCGTTCA CGAGAAATAG 
ACAACAACTC TATATAACTC TTCTCAGGTT TCATAGAAAG CCAAAAGACA 
AAAAATCTTC GCTTCATCTT TTATTTGTGA TCAACATTCT TTTTTTCTTC 
AAAATGTGAT ATGTTTTAAG AAAAACAATG CCAAAAAGAG GGCTTAAGAA 
CAAGAACAGG GTTAGTTCTA GGTCAACCTG TACTTTTCCT TTTTTTTTTT 
TGGACATTTT TCCAACTTCT TACGATTATT TCTGATGATA ATGATTACAA 
CTTTATATAC TGTTTTGGCA ATAATAAGCC AATAATGATA TCTTTCTTCT 
G

Gene models with high sequence identity

  1. AT4G00570.1 [+] show detail - NAD-dependent malic enzyme 2
    E-value: 0; Score: 99.95

 

InterPro Scan Digest

Program Description E-value
superfamily Aminoacid dehydrogenase-like, N-terminal doma 4e-101
superfamily NAD(P)-binding domain 1.2e-89
HMMPanther MALIC ENZYME 1.8e-266
HMMPanther MALIC ENZYME-RELATED 1.8e-266
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
PatternScan Malic enzyme, conserved site 0
HMMPfam Malic enzyme, NAD-binding 1.4e-84
HMMPfam Malic enzyme, N-terminal 8.2e-75
Gene3D NAD(P)-binding domain 1.9e-74

See the detailed result >

AT4G00570.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 2
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-10-A16 introduced

  • F11047 - seed::increased length
  • F19648 - Specific phenotypes were NOT observed.
  • F21211 - Specific phenotypes were NOT observed.
  • F24442 - Specific phenotypes were NOT observed.
  • F29051 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

17 mutant lines found