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RAFL06-10-A16

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda09376)

full reading [+] show sequence

Nucleotide sequence: RAFL06-10-A16 - full reading
GAGAAGAGGA GGAGAACACA AATCCAATTC ACACTGGAAA CTGTTGGGTC 
GGATCCTGAG TGATATTTCG AGACCGATGA TGTGGAAGAA CATTGCTGGG
TTGTCGAAGG CAGCGGCAGC GGCAAGAACA CACGGATCTC GGCGGTGCTT
TTCCACAGCG ATTCCTGGTC CTTGCATCGT CCACAAGCGT GGTGCTGATA
TTCTTCACGA TCCATGGTTC AATAAGGACA CAGGTTTTCC TTTGACTGAG
AGAGATAGAT TAGGGATACG AGGCTTGCTT CCTCCTCGTG TTATGACCTG
TGTACAGCAA TGTGATCGTT TCATTGAGTC GTTTCGTTCG CTAGAGAACA
ACACAAAAGG AGAGCCTGAA AACGTTGTTG CATTAGCTAA ATGGAGGATG
TTGAATAGAT TGCATGACCG TAATGAGACT TTGTACTACC GAGTTCTTAT
TGATAACATC AAAGATTTTG CCCCCATTAT ATACACTCCT ACGGTTGGAC
TTGTTTGTCA GAACTATTCT GGTTTGTATA GAAGACCAAG AGGAATGTAC
TTCAGCGCCA AAGACAAAGG AGAGATGATG TCTATGATTT ATAACTGGCC
TGCTCCGCAG GTAGATATGA TTGTCATCAC TGATGGTAGC CGTATTTTGG
GCTTAGGAGA CCTTGGAGTA CAGGGAATTG GGATTCCTAT TGGAAAGCTC
GACATGTATG TCGCTGCTGC AGGAATCAAT CCACAGAGAG TTTTGCCAAT
TATGTTGGAT GTAGGCACAA ATAATGAAAA GCTCCTACAG AATGATCTCT
ATTTAGGAGT CAGACAACCT AGGTTGGAAG GAGAGGAGTA CCTTGAGATT
ATTGATGAAT TTATGGAAGC TGCATTTACA CGTTGGCCAA AGGCCGTCGT
ACAGTTCGAA GATTTCCAAG CGAAATGGGC TTTCGGAACA TTGGAAAGAT
ATCGGAAGAA ATTTTGCATG TTCAATGATG ATGTCCAGGG AACTGCTGGT
GTTGCACTGG CTGGACTATT GGGAACTGTA AGAGCTCAAG GTCGGCCGAT
ATCCGACTTT GTGAACCAAA AGATTGTTGT GGTGGGAGCT GGAAGTGCTG
GGCTTGGTGT AACTAAGATG GCAGTACAGG CAGTTGCCAG AATGGCAGGT
ATCAGCGAGA GTGAAGCAAC AAAAAACTTT TACCTGATAG ATAAAGATGG
TCTTGTCACC ACGGAGAGGA CAAAACTTGA CCCAGGAGCT GTACTTTTTG
CCAAAAATCC AGCTGAGATA CGCGAGGGAG CAAGTATCGT TGAAGTGGTG
AAGAAAGTGA GGCCACATGT TCTTCTTGGT TTATCTGGAG TTGGTGGTAT
CTTTAATGAA GAAGTTCTCA AGGCGATGCG AGAATCTGAC TCATGCAAGC
CTGCCATTTT TGCCATGTCG AATCCCACTT TGAATGCCGA GTGCACTGCT
GCTGATGCTT TCAAACACGC CGGAGGAAAC ATTGTCTTTG CAAGTGGAAG
TCCATTTGAG AACGTTGAAC TTGAGAATGG TAAAGTAGGA CATGTGAATC
AGGCTAACAA CATGTATTTA TTCCCGGGGA TCGGGCTAGG GACTCTTCTC
TCTGGTGCAC GTATCGTAAC TGATGGAATG CTACAAGCAG CTTCTGAATG
CCTTGCGTCC TACATGACCG ATGAAGAAGT CCAAAAAGGC ATCCTTTACC
CTTCAATCAA CAACATCCGA CACATAACAG CTGAAGTCGG GGCAGCTGTT
CTACGAGCTG CGGTGACTGA TGACATTGCA GAAGGACATG GTGATGTTGG
TCCCAAAGAT CTCAGCCACA TGTCTAAGGA GGATACAGTG AATTACATTA
CACGCAACAT GTGGTTCCCT GTTTACAGCC CTCTCGTTCA CGAGAAATAG
ACAACAACTC TATATAACTC TTCTCAGGTT TCATAGAAAG CCAAAAGACA
AAAAATCTTC GCTTCATCTT TTATTTGTGA TCAACATTCT TTTTTTCTTC
AAAATGTGAT ATGTTTTAAG AAAAACAATG CCAAAAAGAG GGCTTAAGAA
CAAGAACAGG GTTAGTTCTA GGTCAACCTG TACTTTTCCT TTTTTTTTTT
TGGACATTTT TCCAACTTCT TACGATTATT TCTGATGATA ATGATTACAA
CTTTATATAC TGTTTTGGCA ATAATAAGCC AATAATGATA TCTTTCTTCT
G

Gene models with high sequence identity

  1. AT4G00570.1 [+] show detail - NAD-dependent malic enzyme 2
    E-value: 0; Score: 99.95

 

InterPro Scan Digest

Program Description E-value
superfamily Aminoacid dehydrogenase-like, N-terminal doma 4e-101
superfamily NAD(P)-binding domain 1.2e-89
HMMPanther MALIC ENZYME 1.8e-266
HMMPanther MALIC ENZYME-RELATED 1.8e-266
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
FPrintScan Malic oxidoreductase 5.5e-70
PatternScan Malic enzyme, conserved site 0
HMMPfam Malic enzyme, NAD-binding 1.4e-84
HMMPfam Malic enzyme, N-terminal 8.2e-75
Gene3D NAD(P)-binding domain 1.9e-74

See the detailed result >

AT4G00570.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 2
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-10-A16 introduced

  • F11047 - seed::increased length
  • F19648 - Specific phenotypes were NOT observed.
  • F21211 - Specific phenotypes were NOT observed.
  • F24442 - Specific phenotypes were NOT observed.
  • F29051 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

17 mutant lines found