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  3. RAFL06-10-H13

RAFL06-10-H13

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda02987)

full reading [+] show sequence

Nucleotide sequence: RAFL06-10-H13 - full reading 
ACTGTTTCGA ATAGATGGCT TCTATTTCAA CCCCTTTTCC GATTTCTCTC 
CACCCAAAAA CCGTACGATC AAAGCCGTTG AAATTCCGAG TTTTGACCCG 
TCCGATCAAA GCTTCCGGGT CAGAAACTCC TGATCTAACC GTAGCGACAC 
GAACCGGATC CAAAGATCTC CCGATCCGAA ACATACCGGG AAACTACGGT 
TTACCAATCG TAGGACCAAT CAAAGACCGT TGGGATTACT TTTACGACCA 
AGGAGCTGAA GAGTTCTTCA AATCACGAAT CCGTAAATAC AACTCCACGG 
TGTACAGAGT CAACATGCCA CCGGGAGCTT TTATCGCCGA GAATCCACAA 
GTCGTGGCTT TACTCGACGG TAAAAGCTTC CCGGTTTTAT TCGATGTCGA 
TAAAGTCGAA AAGAAAGATC TTTTCACCGG TACTTACATG CCGTCAACGG 
AACTAACCGG AGGCTACCGT ATCCTCTCGT ACCTCGATCC ATCGGAGCCT 
AAACACGAAA AGCTCAAAAA TCTCCTTTTC TTCCTCCTCA AGTCATCTCG 
AAACCGGATC TTCCCTGAGT TTCAAGCTAC TTACTCCGAG CTTTTCGATT 
CTTTGGAGAA AGAGCTTTCC CTTAAAGGGA AAGCGGATTT CGGCGGTTCC 
AGCGACGGAA CCGCCTTTAA TTTCTTGGCT CGAGCTTTCT ACGGGACGAA 
TCCCGCAGAT ACAAAGCTCA AAGCCGACGC TCCGGGTTTG ATCACTAAAT 
GGGTTTTATT CAATCTCCAT CCATTACTCT CTATTGGTTT ACCGAGAGTT 
ATAGAAGAAC CTCTCATCCA TACATTTAGT CTACCACCGG CGTTAGTCAA 
ATCTGATTAC CAGAGACTCT ACGAGTTTTT CTTAGAATCC GCCGGTGAGA 
TTCTCGTTGA AGCCGATAAA TTGGGTATCT CACGAGAAGA AGCTACTCAC 
AATCTTCTCT TCGCCACGTG CTTCAACACG TGGGGTGGGA TGAAGATTTT 
GTTTCCGAAT ATGGTTAAAC GTATCGGGCG GGCGGGTCAT CAAGTTCATA 
ACCGATTAGC GGAGGAGATT AGATCTGTGA TTAAATCCAA CGGCGGAGAA 
CTCACGATGG GAGCGATTGA GAAAATGGAG TTAACCAAAT CAGTGGTTTA 
CGAATGTCTC CGGTTTGAAC CACCGGTTAC GGCTCAATAC GGTAGAGCGA 
AGAAGGATCT GGTTATCGAA AGCCACGACG CGGCGTTTAA AGTCAAAGCC 
GGTGAAATGC TTTACGGTTA TCAACCGTTG GCGACGAGAG ATCCGAAGAT 
TTTTGATCGG GCGGATGAGT TTGTGCCGGA GAGATTCGTC GGAGAAGAAG 
GAGAGAAGCT TTTGAGGCAT GTGTTGTGGT CGAATGGACC GGAGACGGAG 
ACTCCGACGG TGGGGAATAA ACAATGCGCC GGTAAGGATT TTGTTGTTTT 
GGTGGCGAGG TTGTTTGTGA TTGAGATTTT CCGGCGATAT GATTCGTTTG 
ATATTGAGGT TGGTACGTCG CCGTTAGGAA GCTCCGTTAA TTTCTCGTCG 
TTAAGGAAAG CTAGCTTTTA GGAGCCAAGG GTAAATTTGT AATTGTATAT 
GTTCGCACCT GTGTAGTCCA TCTGTTTTCT TGTTCCAGTT TCGTGAATTT 
TGAGAAAATG TTGTAT

Gene models with high sequence identity

  1. AT5G42650.1 [+] show detail - allene oxide synthase
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Cytochrome P450 1e-14
Gene3D Cytochrome P450 4.50001022902866e-40
superfamily Cytochrome P450 5.2e-49
HMMPanther Cytochrome P450 9.80000272130304e-17

See the detailed result >

AT5G42650.1

Model type
Protein coding
Short Description
allene oxide synthase
Curator Summary
Encodes a member of the cytochrome p450 CYP74 gene family that functions as an allene oxide synthase. This enzyme catalyzes dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway. It shows a dual catalytic activity, the major one being a 13-AOS but also expressing a 9-AOS activity.
Computational Description
allene oxide synthase (AOS); FUNCTIONS IN: hydro-lyase activity, allene oxide synthase activity, oxygen binding; INVOLVED IN: in 7 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128); BEST Arabidopsis thaliana protein match is: hydroperoxide lyase 1 (TAIR:AT4G15440.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-10-H13 introduced

  • F14516 - Specific phenotypes were NOT observed.
  • F28604 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

4 mutant lines found