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RAFL06-11-G09

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda03074)

full reading [+] show sequence

Nucleotide sequence: RAFL06-11-G09 - full reading 
GAGAGCTAAA TCATTTACGT TTTGATCGAC AAAAATGGAG TTGATAAACG 
ACTTTCTCAA CCTAACTGCT CCTTTCTTCA CCTTCTTTGG TCTCTGCTTC 
TTCTTGCCTC CTTTTTACTT CTTCAAGTTC TTGCAGTCTA TTTTCTCGAC 
AATTTTCTCT GAAAATCTTT ACGGGAAAGT TGTTCTCATC ACTGGTGCTT 
CCTCCGGTAT AGGCGAGCAA TTGGCATATG AGTACGCATG TAGAGGTGCA 
TGTTTAGCCC TGACCGCCCG AAGGAAGAAC CGTCTAGAGG AAGTGGCAGA 
GATTGCTCGT GAACTCGGAT CTCCCAATGT TGTTACCGTT CATGCTGATG 
TCTCCAAACC TGATGACTGT AGACGAATCG TTGATGACAC CATCACCCAT 
TTTGGCAGAT TGGATCATCT TGTAAATAAT GCTGGGATGA CGCAGATTTC 
AATGTTTGAG AACATTGAAG ATATAACGAG GACAAAAGCA GTTTTGGATA 
CTAACTTCTG GGGATCGGTT TATACCACTC GTGCTGCGCT TCCATACCTT 
AGACAAAGTA ATGGTAAGAT TGTGGCTATG TCGTCTTCCG CAGCGTGGCT 
AACCGCTCCA AGGATGAGTT TTTACAATGC AAGCAAAGCA GCTTTGTTGA 
GCTTCTTTGA GACGATGAGA ATTGAGCTTG GTGGCGATGT ACACATTACA 
ATCGTCACAC CTGGTTATAT CGAGTCGGAG CTCACACAAG GCAAGTACTT 
CTCTGGTGAA GGCGAGTTAA TAGTCAACCA AGACATGAGA GATGTTCAAG 
TAGGACCATT TCCGGTAGCG TCAGCATCAG GATGTGCCAA GTCGATAGTG 
AACGGTGTGT GCCGGAAACA AAGATACGTG ACAGAGCCAT CATGGTTTAA 
GGTGACGTAC CTTTGGAAAG TGCTATGTCC GGAGTTGATT GAGTGGGGTT 
GTCGGTTACT GTACATGACT GGAACTGGTA TGTCCGAGGA TACTGCACTC 
AACAAGAGGA TCATGGACAT TCCTGGTGTA CGTAGTACTC TGTACCCGGA 
ATCTATCAGA ACTCCAGAAA TCAAGTCGGA TTAGAGAGGG GTCAATACAT 
AATAAATGGA TGAGATGTAT CATGAGTATG TGCAATGAGT AATAATTATC 
AAAGAAATGG GTGAAAATGT AGGTTTGTTT TTGTTAATGG TACTTTTGAC 
TATGTATAT

Gene models with high sequence identity

  1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPanther 11BETA-HYDROXYSTEROID DEHYDROGENASE 3.2e-146
HMMPanther Short-chain dehydrogenase/reductase SDR 3.2e-146
Gene3D NAD(P)-binding domain 2.9e-60
superfamily NAD(P)-binding domain 3.1e-61
Coil coiled-coil 0
HMMPfam Short-chain dehydrogenase/reductase SDR 5.5e-32
FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
PatternScan Short-chain dehydrogenase/reductase, conserved site 0

See the detailed result >

AT5G50700.1

Model type
Protein coding
Short Description
hydroxysteroid dehydrogenase 1
Curator Summary
Encodes a hydroxysteroid dehydrogenase HSD1. Two copies of HSD1 (At5g50600 and At5g50700) exist in the Arabidopsis genome as a result of an exact 33-kb duplication on chromosome 5 encompassing seven genes. There are five homologs of HSD1 in Arabidopsis (HSD2-At3g47350, HSD3-At3g47360, HSD4-At5g50590, HSD4-At5g50690 and HSD6-At5g50770; HSD4 has two copies due to the same gene duplication event occurred to HSD1) (Plant Cell Physiology 50:1463). At5g50690 is also named as HSD7 (Plant Physiology 145:87). HSD1 is identified from the proteome of oil bodies from mature seeds. Transcription of HSD1 is specifically and highly induced in oil-accumulating tissues of mature seeds; transcript disappears during germination.
Computational Description
hydroxysteroid dehydrogenase 1 (HSD1); FUNCTIONS IN: oxidoreductase activity, binding, catalytic activity; INVOLVED IN: oxidation reduction, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: seed; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: hydroxysteroid dehydrogenase 1 (TAIR:AT5G50600.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-11-G09 introduced

  • F00736 - rosette leaf::abnormal / cauline leaf::abnormal / sporophyte vegetative stage::decreased speed
  • F24821 - Specific phenotypes were NOT observed.
  • F27532 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

12 mutant lines found