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  3. RAFL06-11-P04

RAFL06-11-P04

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda07545)

full reading [+] show sequence

Nucleotide sequence: RAFL06-11-P04 - full reading 
AACATACACA AAACACTAAA AGTAGAAGAA AAATGGCGAC TCTTAAGGTT 
TCTGATTCTG TTCCTGCTCC TTCTGATGAT GCTGAGCAAT TGAGAACCGC 
TTTTGAAGGA TGGGGTACGA ACGAGGACTT GATCATATCA ATCTTGGCTC 
ACAGAAGTGC TGAACAGAGG AAAGTCATCA GGCAAGCATA CCACGAAACC 
TACGGCGAAG ACCTTCTCAA GACTCTTGAC AAGGAGCTCT CTAACGATTT 
CGAGAGAGCT ATCTTGTTGT GGACTCTTGA ACCCGGTGAG CGTGATGCTT 
TATTGGCTAA TGAAGCTACA AAAAGATGGA CTTCAAGCAA CCAAGTTCTT 
ATGGAAGTTG CTTGCACAAG GACATCAACG CAGCTGCTTC ACGCTAGGCA 
AGCTTACCAT GCTCGCTACA AGAAGTCTCT TGAAGAGGAC GTTGCTCACC 
ACACTACCGG TGACTTCAGA AAGCTTTTGG TTTCTCTTGT TACCTCATAC 
AGGTACGAAG GAGATGAAGT GAACATGACA TTGGCTAAGC AAGAAGCTAA 
GCTGGTCCAT GAGAAAATCA AGGACAAGCA CTACAATGAT GAGGATGTTA 
TTAGAATCTT GTCCACAAGA AGCAAAGCTC AGATCAATGC TACTTTTAAC 
CGTTACCAAG ATGATCATGG CGAGGAAATT CTCAAGAGTC TTGAGGAAGG 
AGATGATGAT GACAAGTTCC TTGCACTTTT GAGGTCAACC ATTCAGTGCT 
TGACAAGACC AGAGCTTTAC TTTGTCGATG TTCTTCGTTC AGCAATCAAC 
AAAACTGGAA CTGATGAAGG AGCACTCACT AGAATTGTGA CCACAAGAGC 
TGAGATTGAC TTGAAGGTCA TTGGAGAGGA GTACCAGCGC AGGAACAGCA 
TTCCTTTGGA GAAAGCTATT ACCAAAGACA CTCGTGGAGA TTACGAGAAG 
ATGCTCGTCG CACTTCTCGG TGAAGATGAT GCTTAATCAA TCAATCCTCC 
ACAGAGAAAC ATAAGCTGCT CTACAGCTTC TGTTATCTCT TATCTCCCTC 
TCTCTCTCTT TGATGAGTTT CAAATCGTTT GATTTTGTTT CTACAAAAAC 
CTTGTTTGTT TCTGTTGTGT GTTTTGAGTT CCTAAATAAT GCAAAAGAGA 
GAGACAGAGA GAACCAGTGT GGTCTCTTAA GTTATATATA TATGAAGAGC 
ATTGGCCC

Gene models with high sequence identity

  1. AT1G35720.1 [+] show detail - annexin 1
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Annexin repeat 1.3e-23
HMMPfam Annexin repeat 1.3e-20
HMMPfam Annexin repeat 1.3e-13
HMMPfam Annexin repeat 7.1e-21
superfamily Annexin 2.9e-102
HMMSmart Annexin repeat 5e-22
HMMSmart Annexin repeat 1.1e-16
HMMSmart Annexin repeat 0.088
HMMSmart Annexin repeat 5.2e-21
FPrintScan Annexin, type plant 8.2e-24
FPrintScan Annexin, type plant 8.2e-24
FPrintScan Annexin, type plant 8.2e-24
FPrintScan Annexin 5e-22
FPrintScan Annexin 5e-22
FPrintScan Annexin 5e-22
FPrintScan Annexin 5e-22
FPrintScan Annexin 5e-22
Gene3D Annexin repeat 4.9e-21
Gene3D Annexin repeat 4.4e-18
Gene3D Annexin repeat 1.8e-24
Gene3D Annexin repeat 3.2e-19
HMMPanther Annexin like protein 1.4e-197
HMMPanther Annexin 1.4e-197
PatternScan Annexin repeat, conserved site 0

See the detailed result >

AT1G35720.1

Model type
Protein coding
Short Description
annexin 1
Curator Summary
Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.
Computational Description
annexin 1 (ANNAT1); FUNCTIONS IN: in 7 functions; INVOLVED IN: in 8 processes; LOCATED IN: in 10 components; EXPRESSED IN: 31 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Annexin like protein (InterPro:IPR015472), Annexin repeat (InterPro:IPR018502), Annexin repeat, conserved site (InterPro:IPR018252), Annexin (InterPro:IPR001464), Annexin, type plant (InterPro:IPR009118); BEST Arabidopsis thaliana protein match is: annexin 2 (TAIR:AT5G65020.1); Has 3809 Blast hits to 2217 proteins in 205 species: Archae - 0; Bacteria - 0; Metazoa - 2893; Fungi - 160; Plants - 482; Viruses - 0; Other Eukaryotes - 274 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-11-P04 introduced

  • F09223 - Specific phenotypes were NOT observed.
  • F18606 - Specific phenotypes were NOT observed.
  • F20848 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

8 mutant lines found