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RAFL06-13-O22

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda09410)

full reading [+] show sequence

Nucleotide sequence: RAFL06-13-O22 - full reading 
ACTCAACCAA TTTCTTCTCA GGTAATACTC GTAAGATTTA TCAACACAAG 
CAAAAAAGAT GGCCCAAAAG CTAGAAGCAA AGGGTGGTGA GATGGGAGAT 
GTATGGGATG ATGGTGTTTA CGAAAATGTT AGAAAGGTAT ATGTAGGGCA 
AGCACAATAC GGTATAGCCT TCGTCAAGTT TGAGTATGTC AATGGTTCTC 
AAGTGGTTGT TGGAGATGAA CATGGAAAGA AGACAGAGCT AGGAGTTGAA 
GAGTTTGAGA TTGATGCGGA TGACTACATC GTATACGTGG AAGGTTACCG 
TGAGAAAGTT AACGATATGA CGTCAGAAAT GATCACGTTT CTTTCTATTA 
AGACTTTTAA AGGCAAAACC TCTCATCCTA TCGAAAAAAG ACCTGGGGTT 
AAGTTTGTGC TACATGGTGG AAAAATCGTT GGGTTTCACG GACGTTCAAC 
AGATGTTTTA CACTCCCTTG GGGCCTATGT TTCTTTGTCA TCCACTATCA 
AATTGCTTGG GAAGTGGATT AAGGTGGAGC AAAAAGGAGA AGGTCCAGGG 
CTAAGATGCT CACATGGCAT AGCACAAGTA GGCAACAAGA TTTACTCCTT 
TGGTGGCGAG TTCACACCAA ATCAGCCCAT CGACAAACAC CTTTACGTCT 
TTGACCTCGA GACCCGGACT TGGTCCATTT CTCCAGCCAC CGGAGACGTT 
CCACACCTCT CTTGTTTAGG TGTCCGGATG GTGTCAGTAG GATCGACCCT 
CTATGTCTTT GGAGGCCGAG ACGCTTCACG CCAATACAAC GGTTTCTACT 
CGTTTGACAC GACCACTAAC GAGTGGAAAC TGCTAACTCC GGTCGAAGAA 
GGACCCACTC CTCGTAGTTT CCACTCAATG GCAGCCGATG AGGAAAACGT 
TTACGTTTTC GGTGGAGTGA GTGCTACGGC ACGACTCAAT ACACTAGACT 
CTTACAACAT CGTTGATAAG AAGTGGTTTC ATTGTTCGAC TCCAGGAGAT 
TCCCTTACCG CAAGAGGAGG AGCAGGGCTC GAAGTGGTGC AAGGGAAGGT 
ATGGGTTGTG TATGGGTTTA ACGGATGTGA AGTAGATGAT GTTCATTACT 
ACGATCCTGT TCAAGACAAG TGGACACAAG TGGAAACATT CGGTGTGAGG 
CCTTCCGAAA GAAGTGTATT CGCTAGTGCG GCTATTGGGA AACACATTGT 
GATTTTTGGA GGTGAAATTG CGATGGACCC GCTAGCTCAC GTGGGTCCAG 
GTCAATTGAC CGATGGAACT TTTGCATTGG ACACAGAGAC GTTGCAATGG 
GAGAGGCTGG ATAAGTTTGG TGGAGAGGAA GAGACTCCGA GTAGCAGAGG 
ATGGACCGCG TCCACGACTG CTACCATTGA TGGTAAGAAA GGACTTGTGA 
TGCATGGTGG TAAAGCTCCA ACCAATGACC GGTTTGATGA TCTCTTCTTT 
TACGGGATTG ACTCTGCCTA ATTAAGGCAG ATTTGGTGAT CATGAACGTT 
TGTGAATGTG TGTGTGTGTG TGTGGGTCTG TGTTTGTGTG TGTAATAAGG 
GAGGGGTATA CCACCATATG ATCCTTCCCC AGCTTGACCA TTGTTTTTTT 
TTTAATATGT TTTTCTTTCT ACATGTTTGT TTCACGTTTC ATGTTGTATC 
CAATAAATTG TGTACTTTCC TAGTAGTAAT CGCCAGAAAA AAAAAAAGG

Gene models with high sequence identity

  1. AT3G16400.1 [+] show detail - nitrile specifier protein 1
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMSmart Kelch repeat type 1 0.41
HMMSmart Kelch repeat type 1 0.26
HMMSmart Kelch repeat type 1 11
superfamily Galactose oxidase/kelch, beta-propeller 8.5e-39
superfamily Mannose-binding lectin 3.8e-38
HMMPfam Mannose-binding lectin 9.3e-41
HMMPfam Kelch repeat type 1 3.3e-07
HMMPfam Kelch repeat type 1 1.7e-06
HMMPfam Kelch repeat type 1 1.1e-07
HMMPfam Kelch repeat type 1 1.2e-07
Gene3D Mannose-binding lectin 1.7e-33
Gene3D Kelch-type beta propeller 1.1e-45
HMMPanther JASMONATE INDUCIBLE PROTEIN-RELATED 0
HMMPanther KELCH REPEAT DOMAIN 0

See the detailed result >

AT3G16400.1

Model type
Protein coding
Short Description
nitrile specifier protein 1
Curator Summary
Encodes a nitrile-specifier protein NSP1 responsible for constitutive and herbivore-induced simple nitrile formation in rosette leaves. NSP1 is one out of five (At3g16400/NSP1, At2g33070/NSP2, At3g16390/NSP3, At3g16410/NSP4 and At5g48180/NSP5) A. thaliana epithiospecifier protein (ESP) homologues that promote simple nitrile, but not epithionitrile or thiocyanate formation.
Computational Description
nitrile specifier protein 1 (NSP1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: glucosinolate catabolic process, response to herbivore, nitrile biosynthetic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: rosette leaf, cotyledon, guard cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Kelch repeat type 1 (InterPro:IPR006652), Galactose oxidase/kelch, beta-propeller (InterPro:IPR011043), Myrosinase-binding related, jacalin-like lectin (InterPro:IPR017388), Mannose-binding lectin (InterPro:IPR001229), Kelch-type beta propeller (InterPro:IPR015915); BEST Arabidopsis thaliana protein match is: nitrile specifier protein 4 (TAIR:AT3G16410.1); Has 12798 Blast hits to 6850 proteins in 422 species: Archae - 13; Bacteria - 486; Metazoa - 7547; Fungi - 708; Plants - 2515; Viruses - 41; Other Eukaryotes - 1488 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-13-O22 introduced

  • F00627 - rosette leaf::desaturated green / rosette leaf::decreased size / rosette leaf::increased width / sporophyte vegetative stage::decreased speed
  • F00721 - rosette leaf::decreased size / rosette leaf::increased width / cauline leaf::abnormal / sporophyte vegetative stage::decreased speed
  • F01826 - Specific phenotypes were NOT observed.
  • F07050 - Specific phenotypes were NOT observed.
  • F07229 - Specific phenotypes were NOT observed.
  • F07836 - Specific phenotypes were NOT observed.
  • F10639 - Specific phenotypes were NOT observed.
  • F13846 - Specific phenotypes were NOT observed.
  • F18511 - Specific phenotypes were NOT observed.
  • F19114 - Specific phenotypes were NOT observed.
  • F21042 - seed::increased size / seed::increased length
  • F21130 - Specific phenotypes were NOT observed.
  • F24141 - Specific phenotypes were NOT observed.
  • F24730 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

17 mutant lines found