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RAFL06-69-D03

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda09445)

full reading [+] show sequence

Nucleotide sequence: RAFL06-69-D03 - full reading
AGTTTCAGCG AAACGATGGA GCTTCTCTGC TCACCAACGT CTCTTTCCTC 
CTCCTTCGCT CTCTCTTCTG CTCTTTTAGT CCCTCGTTCG TTCTCCATGC
CAGGGACAAG GAGATTCATG GTTCTGTGTA GTTCCCAGAA GGAAAGTCAG
ATGACAGTGT CAGTAACTGG AGCAACTGGA TTCATAGGGA GACGATTGGT
TCAGAGACTT CGTGCTGATA ACCACGCTAT CCGGGTCTTA ACACGCTCTA
AATCCAAAGC TGAGCAAATA TTTCCTGCCA AGGACTTCCC AGGTATTGTG
ATTGCAGAAG AATCGGAGTG GAAGAACTGT GTACAAGGCT CTACTGCTGT
TGTGAACTTG GCAGGACTGC CTATTAGTAC AAGGTGGTCT CCCGAGATCA
AGAAAGAGAT TAAGGGCAGC CGGATCCGTG TCACCTCCAA AGTTGTTGAC
TTAATCAATA ATTCACCAGC AGAAGCTAGG CCTACCGTTC TAGTTAGCGC
CACGGCTGTT GGTTACTATG GTACTAGTGA GACAGGAGTA TTCGACGAAA
ATAGTCCATC AGGGAAGGAC TATCTTGCAG AGGTTTGCAG AGAATGGGAA
GGAACTGCAT TGAAAGCAAA TAAGGATGTT AGAGTGGCAC TTATCCGCAT
TGGTGTTGTT CTTGGCAAAG ACGGTGGAGC TTTGGCCATG ATGATACCCT
TTTTCCAAAT GTTTGCTGGA GGACCTCTGG GTTCCGGACA ACAATGGTTC
TCTTGGATTC ACGTGGATGA CTTGGTGAAC TTAATTTACG AGGCCCTCAC
TAATCCATCT TATAAAGGAG TTATTAATGG GACTGCGCCG AATCCGGTTA
GGCTTGGGGA AATGTGCCAG CAGCTAGGCA GTGTTCTTAG CCGACCATCG
TGGCTACCAG TTCCTGACTT TGCACTCAAA GCTTTGCTCG GAGAAGGTGC
CACTGTAGTT CTGGAGGGAC AGAAGGTATT ACCTGTAAGA GCCAAAGAGC
TTGGTTTTGA ATTCAAGTAT AAATACGTGA AGGATGCACT CAGAGCCATT
ATGCAATAAG GTGACACACA TAAGCATCTT TGTAAAATTG GTTTTGATCA
CAGAAAATCC CCAGAAAAAC AAGATATTCT TTTCTTTTCG TCTCTATATC
TTTCTTTTTG AGTAAAACTC GAGAAATGTA TTTATGGTAA AAAGTGTTTT
CGGTGTTATC TCAGAAACCA GAGATCACAG ATGTGTTGAG GATATTATAA
AAAAAAAAAA AATAAAAAAA AAAAAAAA

Gene models with high sequence identity

  1. AT2G21280.1 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMTigr Sugar nucleotide epimerase YfcH, putative 6.8e-173
HMMPanther Sugar nucleotide epimerase YfcH, putative 3.5e-82
Gene3D NAD(P)-binding domain 1.3e-15
HMMPfam NAD-dependent epimerase/dehydratase 1.6e-17
HMMPfam Domain of unknown function DUF1731, C-terminal 2.4e-17
superfamily NAD(P)-binding domain 1.5e-40

See the detailed result >

AT2G21280.1

Model type
Protein coding
Short Description
NAD(P)-binding Rossmann-fold superfamily protein
Curator Summary
A nuclear-encoded, plastid-targeted protein (AtSulA) whose overexpression causes severe yet stochastic plastid (shown in chloroplasts and leucoplasts) division defects. The protein does not appear to interact with either AtFtsZ proteins when studied in a yeast two-hybrid system.
Computational Description
SULA; FUNCTIONS IN: coenzyme binding, binding, catalytic activity; INVOLVED IN: chloroplast fission; LOCATED IN: chloroplast, plastid, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sugar nucleotide epimerase YfcH, putative (InterPro:IPR010099), NAD-dependent epimerase/dehydratase (InterPro:IPR001509), Domain of unknown function DUF1731, C-terminal (InterPro:IPR013549), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G33360.2); Has 4854 Blast hits to 4853 proteins in 1452 species: Archae - 38; Bacteria - 3329; Metazoa - 111; Fungi - 30; Plants - 185; Viruses - 0; Other Eukaryotes - 1161 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-69-D03 introduced

  • F00549 - Specific phenotypes were NOT observed.
  • F08016 - Specific phenotypes were NOT observed.
  • F09306 - Specific phenotypes were NOT observed.
  • F10724 - seed::increased size / seed::increased length
  • F12135 - Specific phenotypes were NOT observed.
  • F17240 - Specific phenotypes were NOT observed.
  • F17536 - seed::increased size
  • F24237 - Specific phenotypes were NOT observed.
  • F25642 - seed::decreased size / seed::decreased length
  • F29902 - Specific phenotypes were NOT observed.
  • F29931 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

12 mutant lines found