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  3. RAFL06-74-I02

RAFL06-74-I02

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda03502)

full reading [+] show sequence

Nucleotide sequence: RAFL06-74-I02 - full reading 
TGAAAGCGGT GAGTCATCTT ACCTTATAAA TGATCACAAA CCCGAGGACT 
GTGAGTTTTC GTGTAGAACA AATCCTTCTC TGAGAAAAAA CAACAGATCC 
GAATTTTATC TTTAATCAGC CGGAAAAAAT GGAGAAAGCG ATCGAGAGAC 
AACGCGTTCT TCTTGAGCAT CTCCGACCTT CTTCTTCTTC TTCGCACAAT 
TACGAGGCTT CTCTATCTGC TTCTGCTTGC TTGGCTGGGG ACAGTGCTGC 
ATATCAGAGG ACCTCTCTCT ATGGAGATGA TGTTGTCATT GTCGCGGCAC 
ATAGGACTCC ACTATGCAAG TCCAAACGTG GCAATTTCAA GGATACATAT 
CCCGATGATT TGCTCGCACC TGTTTTGAGG GCATTGATAG AGAAGACGAA 
TCTAAACCCA AGTGAAGTAG GTGACATTGT TGTGGGTACT GTTTTGGCAC 
CTGGATCTCA GAGAGCCAGT GAATGCAGGA TGGCTGCGTT CTATGCTGGT 
TTCCCTGAAA CCGTGGCTGT CAGAACTGTG AATAGACAGT GCTCATCTGG 
GCTTCAGGCT GTTGCTGATG TAGCCGCTGC CATTAAAGCG GGATTTTATG 
ACATTGGTAT CGGGGCTGGT TTGGAGTCCA TGACTACCAA TCCAATGGCA 
TGGGAAGGGT CAGTCAACCC AGCGGTGAAG AAGTTTGCAC AAGCGCAGAA 
TTGTCTTCTT CCTATGGGTG TTACGTCAGA AAATGTAGCA CAACGCTTTG 
GTGTCTCAAG GCAGGAGCAA GATCAAGCTG CTGTTGACTC GCACAGAAAG 
GCAGCTGCTG CTACTGCTGC TGGTAAATTC AAGGATGAGA TCATTCCTGT 
TAAGACCAAG CTTGTTGACC CGAAGACTGG TGATGAGAAA CCCATTACAG 
TTTCTGTTGA TGATGGTATC CGACCAACCA CAACTCTTGC TTCTCTTGGG 
AAGCTGAAGC CAGTGTTTAA GAAGGATGGC ACCACTACTG CTGGAAATTC 
CAGCCAAGTA AGTGATGGTG CAGGAGCGGT TCTCCTAATG AAGAGAAGTG 
TTGCAATGCA AAAAGGACTT CCCGTTCTTG GTGTATTCAG GACATTTGCT 
GCAGTTGGTG TTGACCCTGC AATCATGGGT ATCGGTCCAG CAGTTGCCAT 
TCCTGCTGCA GTTAAGGCGG CTGGTTTAGA ACTTGATGAC ATCGACTTGT 
TTGAGATCAA TGAGGCATTT GCATCTCAGT TTGTTTATTG CCGTAACAAA 
TTGGGACTTG ACCCAGAGAA AATCAATGTC AACGGAGGTG CAATGGCCAT 
AGGCCATCCT TTGGGCGCTA CAGGAGCGCG TTGTGTTGCT ACATTGTTGC 
ACGAGATGAA ACGCCGTGGT AAAGACTGCC GTTTTGGAGT AGTGTCAATG 
TGCATTGGGA CGGGGATGGG TGCAGCAGCT GTGTTTGAGA GAGGAGATGG 
AGTTGATGAG CTTCGCAACG CAAGGAAAGT TGAAGCGCAA GGTCTTTTGT 
CCAAGGACGC TCGCTAGAGA GGACCATGCA CCAAAACCGT TTTTCACCTC 
ACCTCTCTCT TTTCTGGTCT TCTTGTCACT TTCTTCAGAT ATTATTATAG 
TTTCGAATAA AGCACACAAC CAATGTTTGC CTGAGTCTTG TGTTGTTCTT 
GACCAGGTCA TGTGTTTAGT ATTTTTTTTA AGTGAAGGGG GATAATCTTT 
AAATCTTTTA TATGTAAGAA TATTGTTCTT CTCGAATTTC ATCAGTTGTT 
GCC

Gene models with high sequence identity

  1. AT2G33150.1 [+] show detail - peroxisomal 3-ketoacyl-CoA thiolase 3
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMPfam Thiolase, N-terminal 2.3e-83
HMMPfam Thiolase, C-terminal 1.1e-48
Gene3D Thiolase-like, subgroup 7.7e-63
HMMTigr Thiolase 3.1e-204
PatternScan Thiolase, acyl-enzyme intermediate active site 0
PatternScan Thiolase, active site 0
PatternScan Thiolase, conserved site 0
superfamily Thiolase-like 6.8e-71
superfamily Thiolase-like 3.1e-40
HMMPanther 3-KETOACYL-COA THIOLASE B (BETA- KETOTHIOLASE 1.1e-226
HMMPanther Thiolase 1.1e-226

See the detailed result >

AT2G33150.1

Model type
Protein coding
Short Description
peroxisomal 3-ketoacyl-CoA thiolase 3
Curator Summary
Encodes an organellar (peroxisome, glyoxysome) 3-ketoacyl-CoA thiolase, involved in fatty acid b-oxidation during germination and subsequent seedling growth. Mutants have defects in glyoxysomal fatty acid beta-oxidation. EC2.3.1.16 thiolase.
Computational Description
peroxisomal 3-ketoacyl-CoA thiolase 3 (PKT3); FUNCTIONS IN: acetyl-CoA C-acyltransferase activity; INVOLVED IN: fatty acid beta-oxidation, jasmonic acid biosynthetic process, response to wounding, fatty acid oxidation, glyoxysome organization; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase (InterPro:IPR002155), Thiolase, active site (InterPro:IPR020610), Thiolase, N-terminal (InterPro:IPR020616), Thiolase, conserved site (InterPro:IPR020613), Thiolase, C-terminal (InterPro:IPR020617), Thiolase-like, subgroup (InterPro:IPR016038), Thiolase-like (InterPro:IPR016039), Thiolase, acyl-enzyme intermediate active site (InterPro:IPR020615); BEST Arabidopsis thaliana protein match is: peroxisomal 3-ketoacyl-CoA thiolase 4 (TAIR:AT1G04710.1); Has 22382 Blast hits to 22371 proteins in 2261 species: Archae - 414; Bacteria - 14116; Metazoa - 985; Fungi - 655; Plants - 282; Viruses - 0; Other Eukaryotes - 5930 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-74-I02 introduced

  • F11050 - Specific phenotypes were NOT observed.
  • F12024 - Specific phenotypes were NOT observed.
  • F19316 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

7 mutant lines found