1. Home
  2. Full-length cDNAs
  3. RAFL06-88-G20

RAFL06-88-G20

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda07773)

full reading [+] show sequence

Nucleotide sequence: RAFL06-88-G20 - full reading 
TGAATCAGTT TCGTGTGTTT GGAGAAGAAG AAGAACAGAT CAAATACGAG 
GAGAGATCTC TAAAGAGATT TATCGTTTCA AATTTGTTCA CGTTGAGAAG 
TTTAATTTAG ATTAAACAAC AAAAAGATGG GTTACTGGAA TTCCAAGGTT 
GTTCCAAAAT TCAAGAAGTT ATTCGAGAAA AATAGTGCTA AGAAGGCTGC 
TGCTGCTGAA GCTACCAAGA CCTTTGATGA ATCTAAGGAA ACAATCAACA 
AGGAAATTGA GGAGAAAAAG ACAGAACTCC AACCAAAGGT CGTGGAAACC 
TATGAAGCCA CGTCTGCAGA AGTCAAGGCT TTGGTGAGAG ACCCTAAGGT 
GGCTGGTTTG AAGAAAAACT CAGCGGCTGT GCAGAAGTAC CTCGAGGAGC 
TAGTCAAAAT TGAATTCCCC GGATCAAAAG CGGTGAGTGA GGCTTCGTCT 
AGCTTCGGAG CTGGCTATGT CGCAGGACCG GTCACGTTCA TATTCGAGAA 
GGTATCTGTT TTCCTCCCGG AGGAGGTGAA GACTAAAGAA ATACCGGTGG 
AGGAAGTGAA AGCTGAAGAA CCTGCCAAAA CTGAAGAACC AGCCAAAACC 
GAAGGAACAA GTGGTGAGAA AGAGGAGATT GTTGAAGAGA CAAAGAAAGG 
CGAGACCCCT GAAACCGCGG TCGTGGAGGA GAAGAAACCA GAGGTAGAGG 
AGAAGAAGGA AGAGGCTACT CCGGCTCCGG CAGTGGTTGA AACTCCAGTT 
AAGGAACCGG AGACAACGAC GACAGCGCCA GTGGCTGAAC CACCAAAGCC 
TTGATTTGTT TTCAAGATGG TATGATTACT TCTCTTGTAT GAAAACATCT 
TTGTACGTAA CAAAAAAATG AAAGGAAGAA ACGGAAAGGA ACAAAAAAAA 
AAAACTTATT TTTCATTCTT TTTTTCAATT TGGTTTGTCT TTGTGTGGAT 
AAATCTCTCT GTTTTTTTTT TAATTATTGG GAAAGTCTTT AATGTGTTAT 
TTATTTATAT TCATGTGAAT TTGTAACAAA AATATTTTGT GGATGTTTTC 
TATAGATTTA TTGTTTCAAC

Gene models with high sequence identity

  1. AT4G20260.4 [+] show detail - plasma-membrane associated cation-binding protein 1
    E-value: 0; Score: 99.63

 

InterPro Scan Digest

See the detailed result >

AT4G20260.4

Model type
Protein coding
Short Description
plasma-membrane associated cation-binding protein 1
Curator Summary
Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
Computational Description
plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL06-88-G20 introduced

  • F08825 - Specific phenotypes were NOT observed.
  • F16436 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

5 mutant lines found