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  3. RAFL07-08-B16

RAFL07-08-B16

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda09480)

full reading [+] show sequence

Nucleotide sequence: RAFL07-08-B16 - full reading 
CACTTTGAAC CATTGGTTGG TTTTGATCAA AACCAAAGGA TGTGTGTATG 
TATGTATATA TTCTAACGCT CAAAACTGAT CACTAGCATT CCTAGATTTT 
TCCTTCTTGT GATCACTTGC AGCTATGGCC ACACATGCAG CTCTCGCCGT 
CTCAAGAATC CCGGTCACAC AGCGACTGCA GTCTAAGAGT GCCATTCACT 
CTTTCCCTGC TCAATGCTCC TCCAAGAGGC TAGAAGTCGC TGAATTCTCC 
GGTCTGCGTA TGAGTAGTAT CGGTGGGGAA GCATCTTTCT TCGATGCTGT 
AGCTGCACAA ATCATCCCTA AGGCTGTGAC AACATCAACT CCTGTTAGAG 
GAGAGACAGT GGCGAAACTG AAAGTTGCGA TTAACGGTTT TGGAAGGATT 
GGTAGGAACT TTCTTAGGTG TTGGCATGGT CGTAAAGACT CTCCTCTCGA 
AGTTGTTGTA CTTAACGACA GTGGTGGTGT CAAGAATGCA TCCCACTTGC 
TTAAGTATGA CTCCATGCTT GGAACCTTCA AGGCTGAAGT GAAAATTGTG 
GACAATGAAA CTATTAGTGT TGATGGTAAG CTCATCAAAG TTGTCTCCAA 
CAGAGACCCT CTTAAGCTTC CATGGGCTGA GCTCGGCATT GACATTGTTA 
TCGAGGGAAC AGGAGTGTTT GTTGATGGGC CAGGAGCAGG GAAGCATATC 
CAAGCCGGAG CCTCGAAAGT TATCATCACT GCACCAGCCA AAGGTGCTGA 
TATCCCTACC TATGTTATGG GAGTCAATGA GCAAGACTAT GGTCACGATG 
TCGCTAACAT TATTAGCAAT GCATCTTGCA CCACCAACTG TTTGGCACCT 
TTTGCTAAAG TCTTGGATGA AGAATTTGGA ATTGTCAAGG GGACAATGAC 
AACCACACAC TCCTACACCG GAGACCAAAG GCTTCTAGAT GCATCACACA 
GGGACCTAAG GCGTGCAAGA GCCGCAGCAC TGAACATAGT GCCTACCAGC 
ACAGGAGCAG CCAAGGCGGT GTCATTAGTG TTGCCGCAGC TGAAGGGTAA 
ACTTAACGGC ATTGCACTCC GTGTGCCAAC ACCAAACGTC TCAGTGGTTG 
ACCTTGTTAT AAACGTTGAG AAGAAAGGTT TGACAGCAGA GGATGTGAAC 
GAGGCCTTTA GAAAAGCCGC TAATGGACCG ATGAAAGGCA TTTTAGACGT 
TTGCGATGCG CCTCTTGTCT CTGTTGACTT CAGGTGCTCT GATGTCTCTA 
CCACCATTGA CTCGTCCCTC ACTATGGTTA TGGGTGATGA TATGGTCAAG 
GTGGTTGCTT GGTATGATAA CGAGTGGGGT TACAGCCAAA GAGTGGTGGA 
TTTGGCTCAC CTAGTGGCTA GCAAGTGGCC GGGAGCGGAA GCTGTTGGAA 
GTGGAGATCC TTTGGAGGAT TTCTGCAAGA CAAACCCGGC TGATGAGGAA 
TGCAAAGTCT ATGACTGAAC AACTTTGATC ATTTCTTTCT TTATTTTTCA 
CCAAAACAAT ACTTTGTATC ATGAGTTACT TATTCGAAAT CGTACTATGA 
AAAAAAAAAA AAAAAGAGCT CTTGGATCCA AGCTTTAGCT GAAGGTAGAC 
CATGGAATGA TCCGCCAGTT GTTAAGGAAG CGAATAAGAA GCCTCCGTTG 
GCTCAGGGCG GTTACGGAAA CAACAATAAC AATAACAATG GAGGATGGGA 
TAGTTGGGAT AACGATGATT CTTACAAATC TGATATGAGA AGGAATCAGT 
CAGCGAATGA TTTCAGGGCA TCGGGAAATA GAGAAGGTGC ACATGTGAAG 
TCAAAGTCGT CGGAGGATAT CTACACGCGA TCGCAGCTTG AGGCTTCTGC 
CGCTGGGAAA GAGAGTTTCT TCGCGAGGAG AATGGCGGAG AATGAGTCTA 
AGCCTGAAGG TCTTCCTCCT TCGCAAGGTG GCAAGTATGT AGGATTCGGA 
TCAAGCTCGG CTCCACCACC GAGAAATAAT CAACAGGATG ATGTTTTCTC 
CGTTGTTTCT CAGGGTTTTG GAAGACTGTC TCTGGTTGCT GCATCTGCAG 
CTCAGTCAGC TGCTAGTGTT GTTCAAACAG GAACCAAGGA GTTCACATCC 
AAGGTGAAGG AAGGTGGGTA TGATCACAAG GTGAGTGAAA CTGTAAATGT 
AGTTGCTAAT AAGACAACAG AGATAGGACA TAGGACATGG GGAATCATGA 
AAGGAGTAAT GGCAATGGCG ACACAGAAGG TAGAAGAGTT TACTAAAGAA 
GGATCAACAA GCTGGAATCA GCAGAGTGAA AATGAGGGCA ATGGTTACTA 
CCAGAACTTT GGAAATGGGA ACAAAGCAGC GAATTCATCT GTTGGAGGAG 
GAAGGCCACA ATCATCATCG ACAAGTGGTC ATTACAACAA CTCGCAGAAT 
TCAAATTCTT GGGATAGTTG GGGAGAGAAT GAGAATAAGA AGACAGAAGC 
AGTAGCTCCA AAGGGGTCAT CTGCTTCTAA TGATGATGAT GGTTGGACTG 
GTTGGGATGA TCATGATGCC AAAGATGATG GGTTCGATGG TCATTACCAA 
AGTGCAGGCG ATAAGAAATC TGCTGGTCAC AACGGGAAAT CAGACACCGC 
TTGGACTGGT GGAGGTTTTC TCTAAGGAAT GCCGGTGAGA AGTGTATAAT 
TAATTAAGGC TGGGACCATC GTGGTCTGAA TAGAAGATAA GAATGGTCTT 
CATCTTAAAA CCATGTTCAT CCATACCCAC AGGTCTGCGT TTTTCTTCAT 
AAGTTGGCGG TTTCTTGATA TCTCTTCTTC ATGTGTTAAG GAGTAACGTA 
TTTGTACAAG ATCCATAGCC GATTGTTTGC TAAAGATTTT CGGTTTTAGT 
CTTTCACAAT TTAAATTTAT CTTCAACTGT AACCACAACT GAACATTCTT 
TTTCTCAAAA TTGCCTTGTT CCGTTGTTCG TAAAAAAAAA AAAAAAA

Gene models with high sequence identity

  1. AT1G42970.1 [+] show detail - glyceraldehyde-3-phosphate dehydrogenase B subunit
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
PatternScan Glyceraldehyde 3-phosphate dehydrogenase, active site 0
HMMPanther Glyceraldehyde 3-phosphate dehydrogenase subfamily 3.2e-209
superfamily Glyceraldehyde-3-phosphate dehydrogenase-like 1.2e-71
superfamily NAD(P)-binding domain 4.3e-57
HMMTigr Glyceraldehyde-3-phosphate dehydrogenase, type I 1.5e-191
FPrintScan Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup 1.3e-38
FPrintScan Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup 1.3e-38
FPrintScan Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup 1.3e-38
FPrintScan Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup 1.3e-38
FPrintScan Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup 1.3e-38
HMMPfam Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain 3.9e-67
HMMPfam Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain 1.6e-51
HMMPfam Protein of unknown function CP12 0.000035
HMMSmart Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain 1.6e-76
Gene3D no description 2.8e-69

See the detailed result >

AT1G42970.1

Model type
Protein coding
Short Description
glyceraldehyde-3-phosphate dehydrogenase B subunit
Curator Summary
Encodes chloroplast localized glyceraldehyde-3-phosphate dehydrogenase that can use both NADH and NADPH to reduce 1,3-diphosphate glycerate. It forms A2B2 heterotetramers with GapA forms of the GADPH enzyme. These complexes are active in the light under reducing conditions, but show reduced NADPH-dependent activity in response to oxidized thioredoxins and increased NAD(H)/NADP(H) ratios due to the formation of inactive A8B8 hexadecamers.
Computational Description
glyceraldehyde-3-phosphate dehydrogenase B subunit (GAPB); FUNCTIONS IN: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity, glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity, glyceraldehyde-3-phosphate dehydrogenase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Glyceraldehyde 3-phosphate dehydrogenase subfamily (InterPro:IPR000173), Glyceraldehyde-3-phosphate dehydrogenase, type I (InterPro:IPR006424), Glyceraldehyde 3-phosphate dehydrogenase, active site (InterPro:IPR020830), Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain (InterPro:IPR020829), Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain, subgroup (InterPro:IPR020832), Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain (InterPro:IPR020828), Protein of unknown function CP12 (InterPro:IPR003823); BEST Arabidopsis thaliana protein match is: glyceraldehyde 3-phosphate dehydrogenase A subunit (TAIR:AT3G26650.1); Has 24904 Blast hits to 24897 proteins in 6198 species: Archae - 41; Bacteria - 10859; Metazoa - 2228; Fungi - 2814; Plants - 3753; Viruses - 0; Other Eukaryotes - 5209 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL07-08-B16 introduced

  • F00148 - seed::decreased size
  • F01832 - Specific phenotypes were NOT observed.
  • F08127 - Specific phenotypes were NOT observed.
  • F08839 - seed::increased size / seed::increased length
  • F15505 - Specific phenotypes were NOT observed.
  • F19732 - Specific phenotypes were NOT observed.
  • F20622 - Specific phenotypes were NOT observed.
  • F20644 - Specific phenotypes were NOT observed.
  • F21417 - Specific phenotypes were NOT observed.
  • F21547 - seed::increased size / seed::increased length
  • F22912 - Specific phenotypes were NOT observed.
  • F25947 - seed::increased size
  • F26619 - Specific phenotypes were NOT observed.
  • F28029 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

14 mutant lines found