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RAFL07-10-I24

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda04091)

full reading [+] show sequence

Nucleotide sequence: RAFL07-10-I24 - full reading 
GCTCGCATCT TCTTCTTCTT CACGGAGAAG CAATCGAAGA GAATCAATCA 
ACTAGACAAA AATCGGAAGA AGAAACACAA ACCGAGGTTT TAGAAACATG 
GCGTCTAAGG TAATCTCTGC CACAATCCGC AGAACCCTAA CCAAACCACA 
CGGCACTTTT TCCCGGTGTC GCTACTTATC AACCGCCGCT GCTGCGACGG 
AGGTGAATTA CGAGGATGAA TCGATTATGA TGAAAGGAGT TCGAATTTCA 
GGTAGACCTC TTTACTTAGA TATGCAAGCG ACGACTCCGA TTGATCCTAG 
GGTATTCGAT GCGATGAATG CTTCACAGAT CCATGAGTAT GGGAATCCTC 
ACTCGCGAAC GCATCTCTAC GGCTGGGAAG CTGAGAACGC CGTCGAGAAC 
GCACGAAACC AGGTCGCGAA ACTGATCGAA GCTTCACCGA AGGAGATCGT 
ATTCGTGTCC GGTGCAACGG AGGCGAACAA TATGGCGGTG AAAGGAGTGA 
TGCACTTTTA CAAGGACACG AAGAAACATG TGATAACTAC ACAGACTGAG 
CATAAGTGTG TGCTTGATTC GTGTAGGCAT TTGCAGCAAG AAGGATTTGA 
GGTAACTTAT TTACCTGTGA AAACTGATGG ATTGGTTGAT TTAGAGATGT 
TGAGAGAAGC TATTAGGCCA GACACAGGGC TAGTTTCTAT TATGGCTGTG 
AACAATGAGA TTGGTGTGGT TCAACCTATG GAGGAGATTG GTATGATTTG 
CAAAGAGCAT AATGTTCCGT TTCATACTGA TGCTGCTCAA GCTATTGGGA 
AGATACCTGT TGATGTTAAG AAGTGGAATG TTGCTTTGAT GTCTATGAGT 
GCTCACAAGA TCTATGGACC GAAAGGTGTT GGTGCTTTGT ATGTGAGGAG 
GAGGCCGAGA ATCAGGCTTG AGCCGTTGAT GAATGGTGGA GGTCAGGAGA 
GGGGATTGCG TAGTGGTACG GGGGCTACGC AGCAGATTGT TGGGTTCGGG 
GCTGCTTGTG AGTTGGCTAT GAAGGAGATG GAGTATGATG AGAAGTGGAT 
TAAGGGGTTA CAGGAGAGGT TGCTGAATGG GGTTAGAGAG AAGCTTGATG 
GTGTTGTGGT GAATGGTTCA ATGGATAGTC GATATGTAGG GAATTTGAAT 
TTGTCGTTTG CTTATGTTGA AGGAGAGAGT TTGTTGATGG GATTGAAGGA 
AGTTGCAGTG TCTAGTGGAA GTGCTTGTAC TAGTGCGAGT TTGGAGCCTT 
CTTATGTGTT GAGAGCTTTG GGTGTGGATG AAGACATGGC TCACACTTCG 
ATTAGGTTTG GGATTGGTAG GTTTACCACG AAGGAAGAGA TTGATAAAGC 
GGTCGAGCTT ACGGTTAAAC AAGTTGAGAA GTTGAGGGAA ATGAGCCCGC 
TTTATGAAAT GGTTAAAGAA GGTATCGATA TCAAGAACAT TCAATGGTCT 
CAACACTGAT TCAACAGTTC CACTCAAACT GTGCGGGACA AGAGGGAATC 
TCCAGTCTGT GGTCAGCCGG GGTATATGAG AGCCATACTG AGATGTGAGT 
ATGAACGATG ATATGATCCT GTGTTCTTAC TATGTTCTGT TGGTAAGTTA 
TCTATCTATA TTGCTTTCTA GAGAGAATTG CATGTGAGAG TTTCTTGTGT 
GGTTTTGTAA TAAAACAGAA ACAAAGGTTC ATTTCTGGTT AGATTGTGAT 
ATCCTTTGAA ATTGAATGAT TAATTTTTAG GTGTTGTGAG CTTAGCTTTT 
TTAGCTACGG TAATGGTGAA GTTTGTTTTG TTTTTCTTCT TATTTATGAA 
AATAAAATAC AATTTTATGT GAAAGAAAAA AAAAAAAAAA A

Gene models with high sequence identity

  1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
    E-value: 0; Score: 99.95

 

InterPro Scan Digest

Program Description E-value
HMMPIR Cysteine desulfurase, NifS 1.5e-170
HMMPfam Aminotransferase, class V/Cysteine desulfurase 1.2e-89
Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 1.9e-94
HMMTigr Cysteine desulfurase 8.5e-229
HMMPanther CYSTEINE DESULFURYLASE 1.9e-161
superfamily Pyridoxal phosphate-dependent transferase, major domain 1.5e-114

See the detailed result >

AT5G65720.1

Model type
Protein coding
Short Description
nitrogen fixation S (NIFS)-like 1
Curator Summary
cysteine desulfurase whose activity is dependent on AtSufE activation.
Computational Description
nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL07-10-I24 introduced

  • F11544 - Specific phenotypes were NOT observed.
  • F16944 - Specific phenotypes were NOT observed.
  • F29519 - rosette leaf::abnormal / whole plant::decreased height

Mutant lines with disrupted or induced

9 mutant lines found