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RAFL07-10-I24

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda04091)

full reading [+] show sequence

Nucleotide sequence: RAFL07-10-I24 - full reading
GCTCGCATCT TCTTCTTCTT CACGGAGAAG CAATCGAAGA GAATCAATCA 
ACTAGACAAA AATCGGAAGA AGAAACACAA ACCGAGGTTT TAGAAACATG
GCGTCTAAGG TAATCTCTGC CACAATCCGC AGAACCCTAA CCAAACCACA
CGGCACTTTT TCCCGGTGTC GCTACTTATC AACCGCCGCT GCTGCGACGG
AGGTGAATTA CGAGGATGAA TCGATTATGA TGAAAGGAGT TCGAATTTCA
GGTAGACCTC TTTACTTAGA TATGCAAGCG ACGACTCCGA TTGATCCTAG
GGTATTCGAT GCGATGAATG CTTCACAGAT CCATGAGTAT GGGAATCCTC
ACTCGCGAAC GCATCTCTAC GGCTGGGAAG CTGAGAACGC CGTCGAGAAC
GCACGAAACC AGGTCGCGAA ACTGATCGAA GCTTCACCGA AGGAGATCGT
ATTCGTGTCC GGTGCAACGG AGGCGAACAA TATGGCGGTG AAAGGAGTGA
TGCACTTTTA CAAGGACACG AAGAAACATG TGATAACTAC ACAGACTGAG
CATAAGTGTG TGCTTGATTC GTGTAGGCAT TTGCAGCAAG AAGGATTTGA
GGTAACTTAT TTACCTGTGA AAACTGATGG ATTGGTTGAT TTAGAGATGT
TGAGAGAAGC TATTAGGCCA GACACAGGGC TAGTTTCTAT TATGGCTGTG
AACAATGAGA TTGGTGTGGT TCAACCTATG GAGGAGATTG GTATGATTTG
CAAAGAGCAT AATGTTCCGT TTCATACTGA TGCTGCTCAA GCTATTGGGA
AGATACCTGT TGATGTTAAG AAGTGGAATG TTGCTTTGAT GTCTATGAGT
GCTCACAAGA TCTATGGACC GAAAGGTGTT GGTGCTTTGT ATGTGAGGAG
GAGGCCGAGA ATCAGGCTTG AGCCGTTGAT GAATGGTGGA GGTCAGGAGA
GGGGATTGCG TAGTGGTACG GGGGCTACGC AGCAGATTGT TGGGTTCGGG
GCTGCTTGTG AGTTGGCTAT GAAGGAGATG GAGTATGATG AGAAGTGGAT
TAAGGGGTTA CAGGAGAGGT TGCTGAATGG GGTTAGAGAG AAGCTTGATG
GTGTTGTGGT GAATGGTTCA ATGGATAGTC GATATGTAGG GAATTTGAAT
TTGTCGTTTG CTTATGTTGA AGGAGAGAGT TTGTTGATGG GATTGAAGGA
AGTTGCAGTG TCTAGTGGAA GTGCTTGTAC TAGTGCGAGT TTGGAGCCTT
CTTATGTGTT GAGAGCTTTG GGTGTGGATG AAGACATGGC TCACACTTCG
ATTAGGTTTG GGATTGGTAG GTTTACCACG AAGGAAGAGA TTGATAAAGC
GGTCGAGCTT ACGGTTAAAC AAGTTGAGAA GTTGAGGGAA ATGAGCCCGC
TTTATGAAAT GGTTAAAGAA GGTATCGATA TCAAGAACAT TCAATGGTCT
CAACACTGAT TCAACAGTTC CACTCAAACT GTGCGGGACA AGAGGGAATC
TCCAGTCTGT GGTCAGCCGG GGTATATGAG AGCCATACTG AGATGTGAGT
ATGAACGATG ATATGATCCT GTGTTCTTAC TATGTTCTGT TGGTAAGTTA
TCTATCTATA TTGCTTTCTA GAGAGAATTG CATGTGAGAG TTTCTTGTGT
GGTTTTGTAA TAAAACAGAA ACAAAGGTTC ATTTCTGGTT AGATTGTGAT
ATCCTTTGAA ATTGAATGAT TAATTTTTAG GTGTTGTGAG CTTAGCTTTT
TTAGCTACGG TAATGGTGAA GTTTGTTTTG TTTTTCTTCT TATTTATGAA
AATAAAATAC AATTTTATGT GAAAGAAAAA AAAAAAAAAA A

Gene models with high sequence identity

  1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
    E-value: 0; Score: 99.95

 

InterPro Scan Digest

Program Description E-value
HMMPIR Cysteine desulfurase, NifS 1.5e-170
HMMPfam Aminotransferase, class V/Cysteine desulfurase 1.2e-89
Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 1.9e-94
HMMTigr Cysteine desulfurase 8.5e-229
HMMPanther CYSTEINE DESULFURYLASE 1.9e-161
superfamily Pyridoxal phosphate-dependent transferase, major domain 1.5e-114

See the detailed result >

AT5G65720.1

Model type
Protein coding
Short Description
nitrogen fixation S (NIFS)-like 1
Curator Summary
cysteine desulfurase whose activity is dependent on AtSufE activation.
Computational Description
nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL07-10-I24 introduced

  • F11544 - Specific phenotypes were NOT observed.
  • F16944 - Specific phenotypes were NOT observed.
  • F29519 - rosette leaf::abnormal / whole plant::decreased height

Mutant lines with disrupted or induced

9 mutant lines found