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  3. RAFL08-12-L24

RAFL08-12-L24

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda04739)

full reading [+] show sequence

Nucleotide sequence: RAFL08-12-L24 - full reading 
GGTTTTGGCA CATTACCCTC TTTCCTGTTA AAGTTGTCTC GTCTCCTTCT 
CGTCTCCTTC CTCAAGCATA TTCTCTGCGC TTAAAGTACT TTGATGGCAT 
TGGGACACTT GTGCAGCATC CACTGTTTCC TTAAAATAGG GAAACAAGAA 
GAGCTTCTTG TCCTAGATAT TGGTTTGCCT ATTCCCAGTT TCTTCCTCAT 
TAACTTTATT GGTAGCAGAG CCTCATCCAT CTGGTGAACT TGTGAAGCTT 
CCTCTGTGAT TCTTTTGGCG ATTGGTTAGA AATATGGTTT CTTCTCAGTG 
TTCTGTTGCA AACAAAAACC AGACTGGGAA GCCGTTTCAG AAACATCTAT 
CTTTATCCAT CAGCCCTCCA AAATCTGTCT TGGGTGATAA CCTTGAGCTT 
CAATTCTCTG ATGTCTTTGG ACCAATGCCT GAAGCTAACT CTGAAGAGGC 
CTGTGATGTT GCTTACGACG AGCCTGCTGT TGTCTACAGT CGATCCCACT 
CATTGGTTGG CCCGTCTTTG GTTGTTAGCC ATTCTTTGAA GATGAACAAG 
CTCACTTTAC GAGAAACTGA GGACTCAGTT GACTTGGTGG AGTGTGTTGA 
AGGTGAATCG ATAAAAGAGA ACGATGAATT CTCTGGTAAT GATGACACTG 
ACAGTGAGAA ATCTCCAGAG GAGGTCTCAG GTGTAGTAGG CATTGAGGAC 
TTTGAGGTAT TGAAGGTTGT GGGACAAGGT GCATTTGGAA AAGTGTACCA 
GGTGAGGAAA AAAGACACGT CTGAGATATA CGCGATGAAG GTCATGAGAA 
AAGATAAAAT TGTTGAGAAG AATCATGCTG AATACATGAA AGCCGAGCGC 
GATATTCTAA CCAAAATCGA TCATCCTTTC ATTGTGCAAC TTAAATACTC 
TTTTCAGACC AAATACAGAT TGTATCTTGT TCTTGACTTT ATAAACGGAG 
GTCATCTTTT CTTCCAGCTC TATCACCAAG GGCTTTTCAG GGAGGACTTG 
GCTCGTGTGT ACACTGCAGA AATCGTCTCT GCAGTTTCCC ATCTCCATGA 
GAAAGGCATA ATGCATAGAG ATCTTAAACC TGAAAACATA CTCATGGACG 
TAGATGGCCA TGTGATGTTA ACTGATTTTG GTTTAGCAAA AGAATTTGAA 
GAAAACACAA GATCAAACTC CATGTGTGGG ACTACGGAGT ATATGGCCCC 
TGAGATTGTT CGTGGAAAAG GACATGATAA GGCTGCCGAC TGGTGGAGTG 
TAGGAATCCT TCTGTATGAG ATGCTCACTG GAAAGCCGCC GTTTCTCGGG 
AGCAAAGGAA AGATACAGCA GAAAATCGTT AAGGACAAGA TCAAGCTTCC 
ACAGTTTCTG TCTAATGAAG CTCATGCCTT GCTGAAAGGG CTGCTGCAAA 
AAGAGCCAGA AAGGAGACTG GGAAGTGGAC CGAGCGGAGC AGAGGAGATA 
AAAAAAACAC AAATGGTTCA AGGCGATAAA CTGGAAGAAG CTGGAGGCTA 
GAGAAGTACA ACCAAGTTTC AAGCCGGCGG TTTCGGGAAG ACAATGCATA 
GCTAATTTTG ACAAGTGTTG GACTGACATG TCTGTTTTGG ATTCTCCAGC 
AAGTAGTCCC AACTCTGATG CTAAGGCGAA CCCTTTTACA AACTTCACAT 
ACGTCAGGCC TCCTCATTCA TTCCTTCATC GGACCACATC CAACTTGTAG 
ATGAACTATA ATATGTTACC CCCGCTTCTG TTTTTGTAAT CCGAAACATA 
TGAACATGCA CAAAATCAAC GGTTGTTTGA AGAAACCTAT TTCTCGATTT 
AATATATTAT ACTAGACAAA AAAAAAAAAA AAA

Gene models with high sequence identity

  1. AT3G08720.1 [+] show detail - serine/threonine protein kinase 2
    E-value: 0; Score: 99.94

 

InterPro Scan Digest

Program Description E-value
PatternScan Protein kinase, ATP binding site 0
PatternScan Serine/threonine-protein kinase, active site 0
HMMPfam Serine/threonine-protein kinase-like domain 1.6e-72
HMMPfam Protein kinase, C-terminal 2e-10
superfamily Protein kinase-like domain 8.3e-89
Gene3D no description 2.4e-65
HMMSmart Serine/threonine-protein kinase domain 9.8e-104
HMMSmart Tyrosine-protein kinase, catalytic domain 3.1e-11
HMMSmart AGC-kinase, C-terminal 1.4e-16
ProfileScan Protein kinase, catalytic domain 51.707
ProfileScan AGC-kinase, C-terminal 13.108
HMMPanther RIBOSOMAL PROTEIN S6 KINASE BETA 5.1e-161
HMMPanther SERINE/THREONINE PROTEIN KINASE 5.1e-161
HMMPanther RIBOSOMAL PROTEIN S6 KINASE BETA 1.8e-23
HMMPanther SERINE/THREONINE PROTEIN KINASE 1.8e-23

See the detailed result >

AT3G08720.1

Model type
Protein coding
Short Description
serine/threonine protein kinase 2
Curator Summary
Encodes a ribosomal-protein S6 kinase. Gene expression is induced by cold and salt (NaCl). Activation of AtS6k is regulated by 1-naphthylacetic acid and kinetin, at least in part, via a lipid kinase-dependent pathway. Phosphorylates specifically mammalian and plant S6 at 25 degrees C but not at 37 degrees C. Involved in translational up-regulation of ribosomal proteins.
Computational Description
serine/threonine protein kinase 2 (S6K2); CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), AGC-kinase, C-terminal (InterPro:IPR000961), Protein kinase, C-terminal (InterPro:IPR017892), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: protein-serine kinase 1 (TAIR:AT3G08730.1); Has 133901 Blast hits to 131707 proteins in 4554 species: Archae - 204; Bacteria - 15943; Metazoa - 48718; Fungi - 13429; Plants - 32816; Viruses - 561; Other Eukaryotes - 22230 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL08-12-L24 introduced

  • F22620 - seed::increased size / seed::increased length
  • F26823 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

7 mutant lines found