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RAFL08-17-B19

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz87787)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL08-17-B19 - 5 prime EST 
GAATATCTTC CTTTGTCAAT CTCTTGAGAG TTTTTGCTTC CTCGTCTTCT 
TCGATTCATC AAACAGCGTG TGTTTTTTGT TTAGAGTTGG AAGAGACGAA 
TCGAATTTTG AAGCTTTCTT TTATGTCTGA CAAGATATCT CCGTTTTGTT 
GTCAATCTGG TTGCTTGGAA AACACAGTCC TACATCCAGA ATTAAGGACT 
ACTACTAGTT CCTCCATCAC TTTAGGGTCT CGCATCTTCA AACCGGAGAT 
CCCTCCTCTT TCTCTCCACT TTTTTAGCTA AGAAGAATGG CGCTGCAGAA 
CATTGGTGCT TCCAACCGTA ACGATGCCTT CTACAGGTAC AAGATGCCTA 
AGATGGTTAC CAAAACCGAA GGCAAAGGTA ATGGCATTAA GACCAACATT 
ATCAACAATG TTGAGATTGC CAAAGCCTTG GCTAGACCGC CTTCTTATAC 
GACCAAGTAC TTTGGTTGTG AGCTTGGAGC GCAGTCTAAG TTTGATGAGA 
AGACTGGGAC GTCGCTTGTG AATGGAGCTC ACAACACGTC TAAGCTTGCT 
GGGCTTTTGG AGAATTTTAT TAAGAAGTTT GTTCAGTGTT ATGGATGTGG 
TAACCCCGGA GACTGAGATT ATTATTACA

Gene models with high sequence identity

  1. AT1G77840.1 [+] show detail - Translation initiation factor IF2/IF5
    E-value: 0; Score: 99.84

3 prime EST [+] show sequence

Nucleotide sequence: RAFL08-17-B19 - 3 prime EST 
AGCTTTTGTT CTCAATCGAT CAACTAAAAT TTCTCTGATA CACTTTTAAG 
TTTCTAACAA AATTTCCTTT CAAAATAGAA TAAAAAAGTT TAAACACACT 
TATCTCCTCT AACTTCTTCG AACGTGAGCT TCCTTTAGCA TTGTCTGCCA 
AAGGTCTTTG ACACGATGAC GAAACTTTTT CTGAAACCAA ACTTCAATCT 
CTGAATAAAG CACATTCGGA TTCTGTTCCC AATTCTTTCT CAAACTCTCC 
AAAGTATCAA TCTTCTTCTC TGTCAAAACC ATCACCAAAC TCCCAACTCT 
TTCTAAACCC CAAGCTTCTC TTTCCAACAA CGTTTCAGGT TTCCCAGCCA 
ACAAAGCCCT GAAGCTTTTC AAACAAGTTA ATACCTCTCC TTGAAGCAAA 
GCGCATCCGA AAGCCGCTGC CTCGGTCTCT ATCCT

Gene models with high sequence identity

  1. AT1G33390.1 [+] show detail - RNA helicase family protein
    E-value: 0; Score: 99.77

AT1G33390.1

Model type
Protein coding
Short Description
RNA helicase family protein
Curator Summary
Over-expression of this gene results in stem fasciation. The predicted amino acid sequence reveals the presence of two domains (DEXH-box or DEAD-box helicase and DUF1065 domain) and fragments of two more domains (HrpA domain and HA2 domain).
Computational Description
FASCIATED STEM 4 (FAS4); FUNCTIONS IN: helicase activity, ATP binding, ATP-dependent helicase activity, nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Helicase-associated domain (InterPro:IPR007502), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), Domain of unknown function DUF1605 (InterPro:IPR011709), DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (InterPro:IPR002464), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: ATP-dependent RNA helicase, putative (TAIR:AT3G26560.1); Has 16056 Blast hits to 9082 proteins in 1556 species: Archae - 2; Bacteria - 6415; Metazoa - 3625; Fungi - 2171; Plants - 1181; Viruses - 334; Other Eukaryotes - 2328 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G77840.1

Model type
Protein coding
Short Description
Translation initiation factor IF2/IF5
Computational Description
Translation initiation factor IF2/IF5; FUNCTIONS IN: binding, translation initiation factor activity; INVOLVED IN: translational initiation, regulation of translational initiation; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Translation initiation factor IF2/IF5, N-terminal (InterPro:IPR016189), Translation initiation factor IF2/IF5, zinc-binding (InterPro:IPR016190), eIF4-gamma/eIF5/eIF2-epsilon (InterPro:IPR003307), Translation initiation factor IF2/IF5 (InterPro:IPR002735), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: Translation initiation factor IF2/IF5 (TAIR:AT1G36730.1); Has 4597 Blast hits to 3869 proteins in 471 species: Archae - 198; Bacteria - 234; Metazoa - 1589; Fungi - 711; Plants - 391; Viruses - 12; Other Eukaryotes - 1462 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL08-17-B19 introduced

  • F24236 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

2 mutant lines found

1 mutant line founds