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  3. RAFL09-06-I04

RAFL09-06-I04

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda05092)

full reading [+] show sequence

Nucleotide sequence: RAFL09-06-I04 - full reading 
AGTTATGAGG CTTTAATCGA ATTTGTTGCT TTGCTTTGCG ACTTTCATCT 
TTCCGATTAA TCAAATCCTT TTATCCTCTG AAAAATCTCA ACTTTCGAAG 
AAAACGAAAA TGGCGAGCCT TGTCTACGGG ACGCCGTGGC TTAGGGTTAG 
ATCTTTACCG GAGCTTGCTC CAGCTTTTCT CAGACGGCGT CAATCTTCTT 
TATTTTACTG TTCTCGGCGA AGCTTCGCGG TGGTTGCGTG TTCCACTCCA 
GTCAACAATG GTGGGTCTGT CAGAGTTCGT TTCGCGCCTT CTCCTACTGG 
TAATCTCCAT GTAGGTGGAG CAAGGACTGT TCTTTTCAAC TACTTGTTCG 
CGAGGTCTAA AGGAGGGAAA TTTGTGCTGA GAATTGAAGA TACAGATTTG 
GAGAGATCAA CACGTGAATC TGAAGCAGCT GTTCTTCAGG ATCTCTCATG 
GCTTGGTCTT GATTGGGATG AAGGTCCTGG GGTTAGTGGA GACTTTGGTC 
CTTATCGGCA ATCTGAAAGA AATGCTTTGT ATAAACAATA TGCAGAGAAG 
CTTTTAGAGT CAGGTCATGT CTATCGATGC TTTTGCTCAA GTGAGGAACT 
TGTAAAGATG AAGGAGAATG CAAAGTTGAA ACAGTTGCCT CCAGTATATA 
CCGGGAAATG GGCAACAGCT TCTGATGCTG AAATAGAGCA AGAGTTAGAA 
AAGGGAACAC CTTTTACTTA CCGGTTTCGT GTGCCAAAGG AAGGCTCTTT 
GAAAATTAAT GACCTGATTC GTGGCGAGGT CTGTTGGAAC TTGGATACTC 
TTGGGGATTT TGTGGTAATG AGAAGTAATG GCCAACCCGT TTACAACTTT 
TGTGTTACGG TAGATGATGC TACCATGGCT ATATCACATG TTATAAGGGC 
TGAAGAACAC TTGCCTAATA CTTTGAGGCA GGCTCTAATT TACAAGGCTC 
TTAAGTTCCC GATGCCTCAA TTTGCACATG TTTCTTTAAT TTTAGCTCCG 
GATAGAAGTA AATTATCAAA GCGACATGGG GCAACTTCTG TAGGCCAGTA 
CAGAGAGATG GGATATCTAC CTCAGGGAAT GGTTAACTAT TTGGCACTCT 
TAGGTTGGGG AGACGGGACT GAAAATGAAT TCTTCACACT CGAGGATCTT 
GTTGAAAAAT TCTCGATTGA ACGTGTCAAC AAAAGTGGCG CGATTTTTGA 
TTCAACAAAG TTAAGATGGA TGAATGGTCA ACATCTGAGG GCACTTCCAA 
ATGAGAAACT AACAAAACTT GTTGGTGAGC GATGGAAGAG CGCTGGTATC 
TTAACAGAAT CCGAGGGGAG TTTTGTAAAT GAAGCTGTCG AGCTTCTCAA 
GGATGGGATT GAGTTGGTGA CAGATTCAGA CAAAGTACTT TTGAACTTGC 
TTTCATATCC TCTACACGCT ACATTGGCTA GCCCTGAAGC TAAGCCTGCT 
GTGGAAGACA AACTTCATGA AGTAGCAGCC AGCCTCATAG CTGCTTATGA 
CAGCGGAGAG ATTCCAAGCG CTTTAGAAGA AGGACAAGGT GCTTGGCAGA 
AATGGGTGAA AGCCTTTGGC AAATCCTTGA AACGCAAAGG TAAATCACTT 
TTCATGCCAC TACGAGTGTT GTTAACGGGA AAACTCCATG GTCCTGAGAT 
GGGCACCAGT ATTGTTCTGA TTTACAAAGC TGGAAGTCCT GGTATAGTGG 
TTCCTCAAGC TGGGTTTGTG TCCATGGAGG AACGGTTTAA GATTCTTAGG 
GAGATAGACT GGGAAGCTTT GAACAAAGAT GAGAGTGTGC CTCTTGAATC 
TACAGCCACA GTATCAACCT GAGAAAACTC CCCTTTTTTT TTTTTTTTTT 
TTTTTTCCTT CAATATTTGG TTAGTTGGTG AGAGAAAGAC AGCCCAGAGA 
TTTTGATTCC TCGTGCATTT CTTGTTTCCC GAAACAAATG GCACAAAGAA 
ATTTGTAGAT TATAATGTAT CAGCAAAGCA AACTTAGGAA CAAACCAGAG 
AGCTTGTAAC ATTATCAGGC CAGTTTTAAT TTGCTTTCAA TGCTCTGTTT 
TCTTA

Gene models with high sequence identity

  1. AT5G64050.1 [+] show detail - glutamate tRNA synthetase
    E-value: 0; Score: 99.85

 

InterPro Scan Digest

Program Description E-value
superfamily Nucleotidylyl transferase 9.9e-106
superfamily Aminoacyl-tRNA synthetase, class I, anticodon-binding 1.3e-25
HMMTigr Glutamyl-tRNA synthetase, class Ic, bacterial/mitochondrial 2.4e-184
PatternScan Aminoacyl-tRNA synthetase, class I, conserved site 0
HMMPfam Glutamyl/glutaminyl-tRNA synthetase, class Ic, catalytic domain 9.8e-105
Gene3D Rossmann-like alpha/beta/alpha sandwich fold 1.7e-42
Gene3D Glutamyl/glutaminyl-tRNA synthetase, class Ic, alpha-bundle domain 8.7e-30
Gene3D Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2 0.00038
HMMPanther GLUTAMYL-TRNA SYNTHETASE 1, 2, 3 (GLUTAMATE-- 3.1e-214
HMMPanther Glutamyl/glutaminyl-tRNA synthetase, class Ic 3.1e-214
FPrintScan Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal 5e-26
FPrintScan Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal 5e-26
FPrintScan Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal 5e-26
FPrintScan Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal 5e-26
FPrintScan Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal 5e-26

See the detailed result >

AT5G64050.1

Model type
Protein coding
Short Description
glutamate tRNA synthetase
Curator Summary
Glutamate-tRNA ligase. Targeted to mitochondria and chloroplast. Its inactivation causes developmental arrest of chloroplasts and mitochondria in Nicotiana benthamiana.
Computational Description
glutamate tRNA synthetase (ERS); FUNCTIONS IN: protein binding, glutamate-tRNA ligase activity; INVOLVED IN: chloroplast organization, glutamyl-tRNA aminoacylation, mitochondrion organization, tRNA aminoacylation, ovule development; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminoacyl-tRNA synthetase, class I, conserved site (InterPro:IPR001412), Glutamyl/glutaminyl-tRNA synthetase, class Ic, alpha-bundle domain (InterPro:IPR020061), Glutamyl-tRNA synthetase, class Ic, bacterial/mitochondrial (InterPro:IPR004527), Glutamyl/glutaminyl-tRNA synthetase, class Ic, N-terminal (InterPro:IPR020060), Aminoacyl-tRNA synthetase, class I, anticodon-binding (InterPro:IPR008925), Glutamyl/glutaminyl-tRNA synthetase, class Ic, catalytic domain (InterPro:IPR020058), Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Glutamyl/glutaminyl-tRNA synthetase, class Ic (InterPro:IPR000924), Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2 (InterPro:IPR020751); BEST Arabidopsis thaliana protein match is: Glutamyl/glutaminyl-tRNA synthetase, class Ic (TAIR:AT5G26710.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-06-I04 introduced

  • F08008 - seed::decreased length
  • F14502 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

4 mutant lines found