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  3. RAFL09-14-B02

RAFL09-14-B02

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda05438)

full reading [+] show sequence

Nucleotide sequence: RAFL09-14-B02 - full reading 
ACACACACAA CCACCAAACA CAAAGCTTTT CAAAACCCAG AGAAAATGTT 
CATCGAGAGC TTCAAAGTAG AGAGTCCAAA TGTCAAATAC ACAGAGAATG 
AGATCAATTC GGTGTACGAT TACGAAACAA CAGAGGTCGT CCACGAGAAT 
CGTAACGGTA CATATCAATG GGTCGTGAAG CCAAAGACTG TCAAATACGA 
TTTCAAAACA GACACTCGTG TCCCCAAATT AGGGGTTATG CTTGTTGGTT 
GGGGAGGAAA CAATGGATCA ACACTAACCG CTGGTGTCAT TGCTAACAAA 
GAAGGAATAT CGTGGGCGAC TAAAGACAAA GTGCAACAAG CTAATTACTT 
TGGTTCACTT ACTCAAGCTT CATCGATTCG TGTTGGCTCT TACAATGGTG 
AAGAGATCTA TGCTCCTTTC AAGAGTCTTC TTCCAATGGT GAATCCAGAG 
GATGTTGTGT TTGGTGGTTG GGATATAAGT GACATGAATT TAGCAGACGC 
TATGGCTAGA GCTAGAGTTC TTGATATTGA TCTGCAGAAA CAACTTAGAC 
CTTACATGGA GAACATGATC CCACTTCCTG GTATCTATGA CCCTGATTTC 
ATCGCTGCTA ATCAAGGTTC ACGTGCTAAT AGCGTGATCA AAGGTACCAA 
GAAGGAACAA GTTGATCACA TCATCAAGGA CATGAGGGAG TTTAAGGAGA 
AGAACAAAGT GGATAAGTTG GTTGTGTTAT GGACTGCAAA CACAGAGCGT 
TATAGCAATG TGATTGTAGG ACTTAACGAT ACGACGGAGA ATCTATTGGC 
TTCTGTTGAA AAGGACGAGT CTGAGATCTC GCCGTCTACG TTGTATGCGA 
TTGCTTGTGT TCTTGAAGGC ATTCCTTTCA TCAATGGGAG TCCTCAGAAT 
ACTTTTGTCC CCGGTCTTAT CGAATTGGCT ATATCGAAGA ATTGTTTGAT 
TGGTGGGGAT GATTTCAAGA GTGGTCAGAC TAAGATGAAA TCCGTTTTGG 
TTGATTTCCT TGTTGGAGCT GGCATTAAGC CTACTTCGAT TGTGAGCTAT 
AATCATTTGG GAAACAACGA CGGGATGAAC CTCTCAGCGC CACAGACTTT 
CCGCTCTAAG GAGATTTCAA AGAGCAATGT TGTAGACGAC ATGGTAGCTA 
GTAATGGCAT CCTCTTTGAG CCTGGTGAAC ACCCTGACCA TGTCGTTGTC 
ATCAAGTATG TGCCATATGT TGCGGATAGT AAAAGAGCAA TGGATGAGTA 
CACGTCTGAG ATATTCATGG GAGGGAGGAA CACTATAGTG TTGCATAACA 
CTTGTGAGGA CTCTCTCTTG GCTGCACCAA TTATCTTGGA TCTTGTCCTT 
CTCGCTGAAC TCAGCACCAG AATCCAATTT AAAGCTGAAG GAGAGGGGAA 
GTTTCACTCT TTCCACCCGG TAGCTACCAT ACTCAGTTAC CTCACAAAGG 
CACCTCTTGT ACCGCCCGGA ACACCGGTGG TTAACGCTCT GTCGAAGCAG 
CGGGCAATGC TGGAAAACAT TCTTAGGGCA TGTGTTGGGC TTGCGCCAGA 
GAACAACATG ATCATGGAGT ACAAGTGAAG GATGAGGAAG AAGAAGTAAA 
GGTACCTAAG ATGACCTTAG GATATGAAGG ACACTGCTCC ATTGTTTCAT 
TTATATATAT CATTTTGTAT GTATATTATA TGAATGTGTG TGTGACGGAT 
AATATGGATA AGCTTAGGGT TAACTTGTGT GATATGGATC TGGTCGGTTT 
ATTTGGTGAG AAGATTAGCT AAGCTTTTGG ATCAAATGTG TAAAAGATTT 
AAGTAATTAT GCTTGTGTCT CCTTGTAATA TAACCAAACT TATGATCTAT 
GAATATGGAT TTTC

Gene models with high sequence identity

  1. AT2G22240.1 [+] show detail - myo-inositol-1-phosphate synthase 2
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
Gene3D NAD(P)-binding domain 1.9e-84
HMMPIR Myo-inositol-1-phosphate synthase 2.7e-281
HMMPfam Myo-inositol-1-phosphate synthase 8.2e-118
HMMPfam Myo-inositol-1-phosphate synthase, GAPDH-like 6.2e-46
superfamily NAD(P)-binding domain 3.4e-167
superfamily Glyceraldehyde-3-phosphate dehydrogenase-like 1.1e-45
HMMPanther Myo-inositol-1-phosphate synthase 0

See the detailed result >

AT2G22240.1

Model type
Protein coding
Short Description
myo-inositol-1-phosphate synthase 2
Curator Summary
** Referred to as MIPS1 in Mitsuhashi et al 2008. Myo-inositol-1-phosphate synthase isoform 2. Expressed in leaf, root and silique. Immunolocalization experiments with an antibody recognizing MIPS1, MIPS2, and MIPS3 showed endosperm localization.
Computational Description
myo-inositol-1-phosphate synthase 2 (MIPS2); CONTAINS InterPro DOMAIN/s: Myo-inositol-1-phosphate synthase (InterPro:IPR002587), Myo-inositol-1-phosphate synthase, GAPDH-like (InterPro:IPR013021), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: myo-inositol-1-phosphate synthase 1 (TAIR:AT4G39800.1); Has 1859 Blast hits to 1855 proteins in 512 species: Archae - 101; Bacteria - 453; Metazoa - 126; Fungi - 158; Plants - 764; Viruses - 0; Other Eukaryotes - 257 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-14-B02 introduced

  • F09303 - seed::increased size / seed::increased length
  • F13720 - Specific phenotypes were NOT observed.
  • F22042 - Specific phenotypes were NOT observed.
  • F24737 - rosette leaf::desaturated green / rosette leaf::abnormal / stem::increased thickness / whole plant::decreased height / sporophyte vegetative stage::decreased speed

Mutant lines with disrupted or induced

8 mutant lines found