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  3. RAFL09-14-H22

RAFL09-14-H22

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda01774)

full reading [+] show sequence

Nucleotide sequence: RAFL09-14-H22 - full reading 
AAAAACATAA AGAATCAGCA AAGCAAAATT CGAAGAGACA CAAAAAAACC 
TTCGTTTGTG TCTCAGAATT TACAAAGTTT TGAGTCAGTA GAGAAATGGC 
GGGAATCGTG ACAGAGCCGT GGTCAGTAGC TGAGAACGGA AACCCAAGCA 
TAACGGCGAA AGGATCGAGC AGAGAACTAA GACTTGGAAG AACCGCTCAC 
AACATGTCTT CTTCTTCTTT GAGGAAAAAA TCAGACCTCC GAGTGATTCA 
GAAGGTTCCA TACAAAGGTC TTAAAGATTT TCTCTCTAAT CTCCAAGAAG 
TCATTCTCGG AACAAAGCTT GCCATTCTTT TTCCGGCCAT TCCTGCCGCC 
ATTATTTGCA CCTATTGTGG CGTCAGTCAG CCTTGGATAT TTGGACTTAG 
CTTGCTAGGA CTGACACCTT TGGCTGAGCG AGTCAGCTTT TTGACAGAGC 
AACTAGCTTT CTACACCGGT CCTACATTGG GTGGTCTATT GAACGCAACG 
TGTGGAAACG CGACTGAATT GATAATCGCG ATTCTTGCTT TGACCAATAA 
CAAAGTCGCA GTGGTGAAAT ATTCGCTGCT AGGTTCGATT TTGTCGAACC 
TTTTATTGGT TCTAGGGACT TCACTCTTCT GTGGAGGAAT CGCTAATATC 
CGAAGGGAAC AGCGGTTCGA CCGGAAACAA GCCGATGTGA ACTTCTTCTT 
ACTTCTACTG GGTTTCTTGT GTCACTTGCT GCCATTGTTG GTGGGATACT 
TGAAAAACGG AGAGGCTTCG GCTGCTGTTT TGTCCGACAT GCAACTGAGT 
ATATCGCGAG GCTTCAGTAT TGTTATGTTG ATCAGCTACA TTGCATATCT 
TGTTTTCCAA CTGTGGACTC ACCGCCAATT GTTCGATGCA CAAGAACAGG 
AAGATGAGTA TGATGACGAT GTGGAGCAAG AAACCGCGGT GATTAGTTTT 
TGGAGCGGTT TTGCATGGTT GGTTGGGATG ACACTCGTTA TCGCATTGCT 
ATCGGAGTAT GTTGTAGCCA CGATTGAGGA AGCATCGGAT AAATGGAACT 
TATCAGTGAG TTTCATAAGC ATCATATTGC TTCCTATTGT TGGAAATGCA 
GCTGAACATG CTGGAGCCGT TATTTTTGCC TTTAAGAACA AGCTTGACAT 
ATCTTTGGGA GTTGCGTTAG GCTCTGCGAC TCAGATTGGC TTATTCGTTG 
TCCCCTTGAC CATCATCGTG GCGTGGATTT TGGGAATTAA TATGGATCTC 
AATTTTGGTC CCCTCGAAAC TGGCTGTCTT GCTGTTTCCA TAATCATCAC 
AGCGTTCACA TTACAGGATG GGAGTTCTCA CTACATGAAG GGACTGGTCC 
TCTTGCTTTG CTATTTCATT ATTGCCATCT GTTTCTTCGT CGACAAACTT 
CCCCAGAAAC AAAATGCCAT TCACTTGGGA CATCAAGCGA TGAACAATGT 
CGTCACTGCA ACAGGAGGAG GAGTTTTCTC ATCTTAAACG AAGACAAGTA 
CCAATGTGTT GAAACAAAAT TTCCAAAGCG AGAGACAATG GAGAAAGGAA 
AACAAAAACC AATTTTCTTG ATTCATTTAT AAATATTGAA AGATTTGTCA 
GAGAGATTCC TGAGATTCAG TGAGTATTAT TTCATTTTTC AAAAATGTTG 
GGTGTCTTGA TGAAGATGAG TTTTGAGAAC TTTATAAATT CTTCTTGTGG 
GTGGGTTTTG CAATGAACTA TCTATGAATG TCTTGTGTCA AATGTTCTAT 
CTCTTTTTAT TAATGGTTTT ATCATTTTTT CTGAGG

Gene models with high sequence identity

  1. AT2G38170.1 [+] show detail - cation exchanger 1
    E-value: 0; Score: 100.00

 

InterPro Scan Digest

Program Description E-value
HMMTigr Calcium/proton exchanger 3.7e-237
HMMTigr Calcium/proton exchanger superfamily 2.5e-88
HMMPfam Sodium/calcium exchanger membrane region 6.1e-22
HMMPfam Sodium/calcium exchanger membrane region 2.1e-18

See the detailed result >

AT2G38170.1

Model type
Protein coding
Short Description
cation exchanger 1
Curator Summary
Encodes a high affinity vacuolar calcium antiporter. The residue His 338 is critical to Ca2+ transport activity. Disruption of CAX1 reduces manganese and zinc of shoot tissue and results in a decrease in the activity of vacuolar V-type proton ATPase.
Computational Description
cation exchanger 1 (CAX1); FUNCTIONS IN: calcium:cation antiporter activity, calcium ion transmembrane transporter activity, calcium:hydrogen antiporter activity; INVOLVED IN: cold acclimation, response to salt stress, cellular zinc ion homeostasis, cellular manganese ion homeostasis, calcium ion transport; LOCATED IN: plant-type vacuole membrane, vacuolar membrane, vacuole, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sodium/calcium exchanger membrane region (InterPro:IPR004837), Calcium/proton exchanger superfamily (InterPro:IPR004798), Calcium/proton exchanger (InterPro:IPR004713); BEST Arabidopsis thaliana protein match is: cation exchanger 3 (TAIR:AT3G51860.1); Has 3042 Blast hits to 2882 proteins in 950 species: Archae - 18; Bacteria - 1703; Metazoa - 62; Fungi - 722; Plants - 217; Viruses - 0; Other Eukaryotes - 320 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-14-H22 introduced

  • F00524 - Specific phenotypes were NOT observed.
  • F07244 - Specific phenotypes were NOT observed.
  • F08005 - Specific phenotypes were NOT observed.
  • F09401 - cauline leaf::decreased size / cauline leaf::present in fewer numbers in organism / stem::abnormal
  • F10436 - rosette leaf::increased size / cauline leaf::increased size / cauline leaf::epinastic / cauline leaf::dark green / whole plant::increased height / stem internode::decreased length / rosette leaf::dark green / rosette leaf::increased size / rosette leaf::present in greater numbers in organism / stem::increased thickness / cauline leaf::dark green
  • F16928 - Specific phenotypes were NOT observed.
  • F17629 - Specific phenotypes were NOT observed.
  • F17916 - stem::present in fewer numbers in organism / stem::abnormal / rosette leaf::present in greater numbers in organism / rosette leaf::abnormal / cauline leaf::decreased size / cauline leaf::dark green / stem::present in fewer numbers in organism
  • F19015 - Specific phenotypes were NOT observed.
  • F19121 - Specific phenotypes were NOT observed.
  • F22608 - Specific phenotypes were NOT observed.
  • F26849 - Specific phenotypes were NOT observed.
  • F28605 - seed::increased size / seed::increased length
  • F30504 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

15 mutant lines found