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  3. RAFL09-21-G21

RAFL09-21-G21

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda08023)

full reading [+] show sequence

Nucleotide sequence: RAFL09-21-G21 - full reading 
AAAAACTTGA GAAGATGATG GCTGATGACA CTGAACTCGT CAGTAACACT 
GACCAGAAGA TGGAAGACGC TTCAGCCGAA AACCCTGCCC GAGCTGATCC 
CCCCTCATCG GATGATTCCG GCGATTCTGA TTCCGACTCA GAAGATGAAG 
CCGAGTCGAA TCACCAGATT GTAACCTTGG AGTCGGAGCT CTCCGCTAAT 
CCTTACAACT ACGATGCTTA TGTCCAATAC ATTAAGCTTC TCAGGAAAAC 
AGCCAACCTT GAAAAGCTAA GACAAGCAAG GGAAGCTATG AGTGCCATAT 
TTCCGTTGAG TCCTTCCTTG TGGCTTGAAT GGGCAAGGGA TGAAGCGTCT 
TTAGCCGCTA GTGAGAATGT GCCTGAAATT GTGATGCTTT ATGAACGGGG 
ACTTTCTGAT TATCAGTCTG TATCTCTTTG GTGTGACTAC CTGAGCTTTA 
TGCTAGAATT TGATCCATCA GTGCGTGGAT ACCCATCTGA AGGTATTTCC 
AAAATGCGGA GTCTTTTTGA ACGTGCTATT CCGGCTGCGG GCTTCCATGT 
TACTGAAGGC AATAGAATAT GGGAAGGATA TAGAGAATTT GAGCAAGGTG 
TCTTAGCCAC TATCGACGAG GCTGACATTG AGGAGAGAAA TAAGCAGATA 
CAAAGGATCA GAAGCATATT CCACCGTCAC CTGTCTGTTC CTCTAGAAAA 
CTTGAGCTCC ACCCTTATTG CTTACAAGAC TTGGGAGTTG GAACAGGGCA 
TTGACCTTGA TATTGGATCT GATGATTTGA GTAAGGTTTC TCATCAGGTT 
GCTGTTGCAA ACAAGAAGGC ACAGCAGATG TATAGTGAAC GTGCCCATCT 
TGAAGAAAAC ATATCTAAGC AAGATTTATC TGACACGGAA AAGTTTCAGG 
AATTCATGAA TTATATAAAG TTTGAGAAGA CTTCTGGAGA TCCTACCCGA 
GTTCAAGCTA TATATGAACG TGCTGTGGCT GAATACCCGG TTTCAAGTGA 
CTTGTGGATA GACTACACCG TGTATCTTGA TAAAACTTTG AAGGTTGGAA 
AAGCTATAAC GCATGCCTAT TCTAGGGCGA CTAGAAGTTG TCCATGGACT 
GGAGATCTTT GGGCACGTTA TTTACTTGCT TTAGAGCGTG GTTCTGCATC 
CGAGAAAGAG ATTTATGATG TCTTTGAGAA GTCATTGCAG TGCACATTTT 
CCTCCTTTGA AGAGTACCTG GATTTATACC TCACTCGAGT AGATGGCCTG 
AGAAGGAGAA TGTTATCAAC TAGGATGCTA GAGGCTTTGG ACTATTCTCT 
GATAAGAGAA ACTTTTCAGC AAGCATCAGA TTACCTAACG CCTCACATGC 
AAAATACAGA CAGTTTGCTA CATTTGCATA CTTATTGGGC TAACTTGGAA 
CTTAATATTG GAAAAGATCT AGCTGGCGCA CGAGGTGTTT GGGATAGCTT 
TCTGAAGAAG AGTGGTGGGA TGCTAGCAGC CTGGCATGCA TACATTGATA 
TGGAAGTGCA TTTGGGGCAT ATAAAGGAAG CTAGGTCTAT TTACCGGAGG 
TGCTATACAA GAAAGTTTGA TGGAACTGGC TCAGAAGACA TATGCAAAGG 
ATGGTTGCGC TTTGAGAGGG AGCATGGGGA TTTGGAACAT TTTGACCTAG 
CAGTTCAAAA GGTTATGCCA CGCTTGGAAG AGCTACAATT GATGAGGTTA 
CAACAAGAGT CTACACCAGT CAAACCGTCA GCTGGTCTGA AAGAGCATAG 
CTCACAAAAG AGAAAAGCAG AACAGAATGT AGAAGAAGAG TCTTTGGCTA 
AGCGGCAGAA GAGGAAAAGT CAAAAAGAAG TGGACCTAGG TGGTCAGAGT 
GCAACTGTAC CAGCAACAAA AAACGTTAAA GCAGAGAATG GAAAAACAGC 
TGATTCAGAT AAAGAAGAGA CAGAAGATGT CAAACCACTG AAGCCGAAAG 
TTTATAGAGA CGAGTGTACA GCTTTTATTT CGAATCTCAG TGTCAAGGCA 
CAAGAGGAAG ATATACGTAA ATTCTTTGGT GATGATGGTG GGGTTGATTC 
CATTCGCATT CTCCATCACA AAGACACTGG AAAACCAAGG GGACTGGCAT 
ATGCTGATTT TGTGGATGAT GAACACCTTG CAGCAGCTAT AGCAAAGAAC 
AGAAAGATGT TTTTTGGTAA GAAGATCAGC ATAGCACGGT CAAATCCAAA 
GAAGGGTAAA AAAGAATTCA CTCGGCGTGG AAATGATGGT TCTGGGAATT 
CAAAGGACCC TTCCCTGATT TCTGAGAAGG CGAAAGCACC ACTTGGTGGA 
GAAACTGAGG GTGAAAGGAA AGGAAATGAA GTGGAAGTGA GGGGTAAGAA 
CACGTTTGCT GTCCCAAGAA ATGTTAAACC ACTCGGCTAC ACGACACCTA 
AACCCAGCGC AGATGAGACA CCAAAATCAA ATGATGAGTT CAGGAATATG 
TTCCTTAAAA AGTGAGACTT TTGCTTGGTC CCTATCTTCC TTCCCTTAGA 
TACTTGTCTT GTTCTACTTT TCGGAAGTAA TCTTATGTTG TAGACCAGCA 
ACATAGGATC TGCTACTCTC TTTTGTCAAG ATAACTAGTT GAGCTTCTAT 
ATAACTAATA TATAAATTTA AGTCTTGC

Gene models with high sequence identity

  1. AT4G24270.1 [+] show detail - EMBRYO DEFECTIVE 140
    E-value: 0; Score: 99.89

 

InterPro Scan Digest

Program Description E-value
ProfileScan RNA recognition motif, RNP-1 13.891
superfamily RNA-binding domain, RBD 8.5e-19
superfamily TPR-like 5.3e-09
Gene3D Nucleotide-binding, alpha-beta plait 9.5e-17
Coil coiled-coil 0
Coil coiled-coil 0
HMMSmart RNA-processing protein, HAT helix 140
HMMSmart RNA-processing protein, HAT helix 12
HMMSmart RNA-processing protein, HAT helix 12
HMMSmart RNA-processing protein, HAT helix 230
HMMSmart RNA-processing protein, HAT helix 0.0005
HMMSmart RNA-processing protein, HAT helix 47
HMMSmart RNA-processing protein, HAT helix 43
HMMSmart RNA-processing protein, HAT helix 26
HMMSmart RNA recognition motif, RNP-1 4.6e-16
HMMPfam RNA recognition motif, RNP-1 1.2e-11
HMMPfam Lsm interaction 2.5e-08
HMMPanther RIBONUCLEOPROTEIN-RELATED 6.3e-188

See the detailed result >

AT4G24270.1

Model type
Protein coding
Short Description
EMBRYO DEFECTIVE 140
Curator Summary
A locus involved in embryogenesis. Mutations in this locus result in embryo lethality.
Computational Description
EMBRYO DEFECTIVE 140 (EMB140); FUNCTIONS IN: RNA binding, nucleotide binding, nucleic acid binding; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: intracellular; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Lsm interaction (InterPro:IPR008669), RNA-processing protein, HAT helix (InterPro:IPR003107), RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: crooked neck protein, putative / cell cycle protein, putative (TAIR:AT5G45990.1); Has 6865 Blast hits to 6296 proteins in 569 species: Archae - 0; Bacteria - 876; Metazoa - 2538; Fungi - 1681; Plants - 1001; Viruses - 0; Other Eukaryotes - 769 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-21-G21 introduced

  • F14207 - stem::increased thickness / stem::desaturated green / stem::abnormal / cauline leaf::desaturated green / stem::abnormal
  • F17246 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

3 mutant lines found