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  3. RAFL09-27-M17

RAFL09-27-M17

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda17677)

full reading [+] show sequence

Nucleotide sequence: RAFL09-27-M17 - full reading 
GATTGATGTT CAGTGAACTT GCACCAAACT TCACTTCTTC TATACTTCTT 
CATAGCCACA AACTCAGTTC TTTGCAAGAA ACACAAACTT AAGTATTCAA 
AATATCGTCA TCATGTTCTC AAGATTCCAT GCTCTGTTTC TTCTCCTTGT 
TCTTTCAGTA AGAACATATA AATGTGTATC TTCATCTTCT TCTTCTTCTT 
CTTCTTTCTC ATTCTCTTCA TTTTCTTCTT CGTCTTCTTC TCAAACTCTT 
GTCTTGCCTC TAAAGACCCG AATAACCCCA ACGGATCATC GACCCACCGA 
CAAACTTCAT TTCCACCACA ACGTCACTCT CACCGTTACT CTCACCGTCG 
GTACTCCTCC TCAAAACATC TCCATGGTCA TTGACACTGG CAGTGAACTC 
TCCTGGCTTC GTTGCAACCG TTCCTCAAAC CCAAACCCGG TTAACAATTT 
TGACCCGACC CGTTCCTCCT CTTATTCCCC AATCCCCTGT TCCTCACCCA 
CTTGTCGTAC CCGGACCCGA GACTTCCTCA TACCCGCTTC TTGCGATTCA 
GACAAACTCT GCCACGCAAC TCTATCCTAC GCCGACGCTT CCTCCTCCGA 
AGGTAACCTC GCCGCCGAGA TTTTCCACTT CGGAAACTCC ACCAACGACT 
CCAATCTCAT TTTCGGGTGT ATGGGTTCCG TATCCGGATC CGACCCGGAA 
GAAGATACCA AAACAACTGG GTTATTGGGT ATGAACCGTG GTTCCCTCTC 
TTTTATCTCT CAAATGGGTT TCCCCAAATT CTCTTATTGC ATCTCCGGCA 
CCGACGACTT CCCGGGGTTT CTTCTCCTCG GCGACTCTAA CTTCACGTGG 
TTAACGCCGT TAAATTACAC GCCGTTAATT CGTATCTCTA CGCCGTTACC 
TTACTTCGAC CGTGTAGCTT ACACCGTCCA GCTCACGGGT ATTAAAGTTA 
ACGGTAAGCT TTTACCCATC CCTAAATCGG TTCTCGTACC GGATCATACC 
GGTGCTGGTC AAACCATGGT TGATTCCGGT ACTCAGTTTA CTTTCCTGCT 
TGGACCGGTT TACACCGCAC TTCGATCCCA TTTCTTAAAC CGGACAAACG 
GAATCTTAAC GGTTTACGAA GACCCAGATT TTGTTTTCCA AGGAACCATG 
GATTTGTGTT ATCGGATTTC ACCGGTTCGT ATACGGTCCG GAATTCTCCA 
CCGTTTACCA ACAGTTTCTC TGGTTTTCGA AGGAGCCGAG ATAGCGGTTT 
CGGGTCAGCC GCTATTGTAC CGAGTCCCGC ATCTCACGGT TGGAAACGAT 
TCGGTTTATT GTTTCACATT TGGAAACTCC GACCTTATGG GAATGGAGGC 
TTACGTGATT GGACATCATC ATCAGCAGAA CATGTGGATC GAATTTGATC 
TACAAAGATC AAGAATCGGT TTAGCTCCGG TAGAATGTGA TGTATCGGGT 
CAGAGACTCG GAATCGGATC GTAAACCATA GATAACCAGT GTGTAATTAT 
AGTTTTTTGC CTACGTAGAA ATGGTTTCTC TAGTAGAAAG GTATGCACGT 
GACTACAATG AGATTTGTGT TACCGACACG AGCGGGTGTG TAATAACTTC 
GAGTTTGGTA GTAGTTAGTT AGTTGTCTCT GTTTGCTTTT GACATGGTCT 
TTTCTTTGCG TAATTAATTT TTAGCTGAGA GTTATATCAA AAAAAAAA

Gene models with high sequence identity

  1. AT5G02190.1 [+] show detail - Eukaryotic aspartyl protease family protein
    E-value: 0; Score: 99.94

 

InterPro Scan Digest

Program Description E-value
Gene3D Peptidase aspartic, catalytic 3.6e-31
superfamily Peptidase aspartic 1.2e-83
HMMPanther CHLOROPLAST NUCLEIOD DNA-BINDING-RELATED 1e-99
HMMPanther Peptidase A1 1e-99
HMMPfam Peptidase A1 0.0000000000024
PatternScan Peptidase aspartic, active site 0

See the detailed result >

AT5G02190.1

Model type
Protein coding
Short Description
Eukaryotic aspartyl protease family protein
Curator Summary
encodes an aspartic protease, has an important role in determining cell fate during embryonic development and in reproduction processes. The loss-of-function mutation of PCS1 causes degeneration of both male and female gametophytes and excessive cell death of developing embryos during torpedo stage.
Computational Description
PROMOTION OF CELL SURVIVAL 1 (PCS1); CONTAINS InterPro DOMAIN/s: Peptidase aspartic (InterPro:IPR021109), Peptidase aspartic, catalytic (InterPro:IPR009007), Peptidase A1 (InterPro:IPR001461), Peptidase aspartic, active site (InterPro:IPR001969); BEST Arabidopsis thaliana protein match is: Eukaryotic aspartyl protease family protein (TAIR:AT2G39710.1); Has 2066 Blast hits to 2053 proteins in 143 species: Archae - 0; Bacteria - 0; Metazoa - 95; Fungi - 96; Plants - 1822; Viruses - 0; Other Eukaryotes - 53 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-27-M17 introduced

  • F10709 - Specific phenotypes were NOT observed.
  • F15713 - cauline leaf::epinastic / whole plant::increased height / stem::abnormal / stem internode::decreased length

Mutant lines with disrupted or induced

5 mutant lines found