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  3. RAFL09-54-N06

RAFL09-54-N06

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda17759)

full reading [+] show sequence

Nucleotide sequence: RAFL09-54-N06 - full reading 
GAACAAACCA ACTTCAGCGA CAAGATAACA AAACGCTTTC ACACGATCGG 
ATAAAATGGC GGAGAAGAGC TTTAAGTACA TCATCCTCGG CGGCGGCGTC 
TCTGCCGGAT ACGCAGCTAA GGAGTTTGCC AATCAAGGGG TTCAACCAGG 
AGAATTGGCT GTTATCTCGA AAGAGGCGGT GGCTCCATAT GAACGCCCTG 
CACTTAGCAA GGGGTATTTG TTTCCTGAAG GGGCTGCTAG ACTTCCAGGT 
TTCCATTGCT GTGTTGGTAG TGGTGGAGAA AAACTGCTTC CTGAATCCTA 
CAAGCAGAAA GGAATTGAGT TGATACTAAG CACAGAAATA GTGAAAGCTG 
ATCTCTCTGC CAAGAGTCTT GTCAGTGCAA CTGGGGATGT CTTCAAGTAT 
CAGACGCTCA TAATTGCAAC TGGTTCTACT GTTCTCAGAT TGACTGATTT 
TGGAGTAAAA GGTGCAGACT CTAAGAATAT CCTCTATCTG AGAGAGATTG 
ATGATGCCGA CAAATTGGTT GAAGCAATTA AAGCTAAGAA AGGTGGAAAG 
GCTGTAGTTG TTGGCGGAGG TTACATTGGT CTTGAGCTTA GTGCAGTTTT 
AAGGATCAAC AATTTAGATG TCACTATGGT TTTCCCTGAA CCTTGGTGCA 
TGCCTAGGCT TTTCACCGCC GATATTGCTG CTTTCTATGA GACTTACTAT 
ACAAACAAGG GAGTGAAGAT CATTAAAGGA ACAGTGGCAT CTGGGTTCAC 
AGCTCAGCCA AATGGAGAGG TGAAGGAAGT ACAACTCAAG GATGGGAGGA 
CATTGGAAGC CGACATTGTG ATAGTTGGGG TTGGTGCAAA ACCATTGACA 
TCCTTATTCA AGGGACAGGT CGAGGAAGAT AAAGGTGGAA TTAAGACCGA 
TGCATTCTTC AAAACAAGTG TTCCAGATGT TTACGCTGTT GGTGACGTTG 
CTACTTTCCC TTTGAAAATG TACGGAGACG TGAGAAGGGT CGAGCATGTT 
GACCATTCCC GCAAATCAGC AGAGCAAGCT GTTAAGGCGA TCAAAGCGGC 
TGAGGGAGGC GCAGCGGTGG AGGAATACGA CTATCTTCCA TTCTTCTACT 
CTCGCTCCTT TGATCTCTCA TGGCAGTTCT ACGGAGACAA CGTAGGAGAC 
TCTGTGTTAT TTGGAGACAG CAATCCATCA AACCCAAAAC CAAGATTTGG 
AGCATATTGG GTTCAAGGTG GTAAAGTGGT TGGAGCATTC ATGGAGGGAG 
GTAGTGGTGA TGAGAACAAA GCCTTGGCTA AAGTCGCCAA AGCTCGACCT 
TCTGCAGAGA GTCTCGACGA GCTCGTGAAA CAAGGAATTT CCTTCGCAGC 
TAAGATCTGA GTGAAACACA ACCACCAAAA CAAACTCACG TTTTCTCTTC 
TATGTTAAAA TAAAAACTTG TTTAGCTATA ATAAGTTGTG TGTTTGTATT 
GTCTCTCTAA ACTCCATGAG AAGAGTCTGG CAGAGGAAAC CCTGAACCAC 
ATTCACATTT TAGATCATGT GTTTGGTTTT CTGTGTTCCA AAAAAAAAA

Gene models with high sequence identity

  1. AT3G52880.1 [+] show detail - monodehydroascorbate reductase 1
    E-value: 0; Score: 99.87

 

InterPro Scan Digest

Program Description E-value
HMMPfam FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.5e-33
HMMPfam Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region 8.9e-16
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
FPrintScan PNDRDTASEI 3.5e-17
FPrintScan PNDRDTASEI 3.5e-17
FPrintScan PNDRDTASEI 3.5e-17
FPrintScan PNDRDTASEI 3.5e-17
Gene3D no description 1.9e-14
Gene3D no description 1.5e-36
superfamily FAD/NAD(P)-binding domain 4.9e-38
superfamily FAD/NAD-linked reductase, dimerisation 2.2e-17
HMMPanther MONODEHYDROASCORBATE REDUCTASE (NADH) 6.4e-188
HMMPanther DISULFIDE OXIDOREDUCTASE 6.4e-188

See the detailed result >

AT3G52880.1

Model type
Protein coding
Short Description
monodehydroascorbate reductase 1
Curator Summary
Encodes a peroxisomal monodehydroascorbate reductase, involved in the ascorbate-glutathione cycle which removes toxic H2O2
Computational Description
monodehydroascorbate reductase 1 (MDAR1); FUNCTIONS IN: monodehydroascorbate reductase (NADH) activity; INVOLVED IN: response to cadmium ion, peptidyl-cysteine S-nitrosylation, hydrogen peroxide catabolic process; LOCATED IN: peroxisomal matrix, apoplast, chloroplast, peroxisome, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (InterPro:IPR013027), FAD/NAD-linked reductase, dimerisation (InterPro:IPR016156), Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (InterPro:IPR001327); BEST Arabidopsis thaliana protein match is: Pyridine nucleotide-disulphide oxidoreductase family protein (TAIR:AT5G03630.1); Has 31553 Blast hits to 31492 proteins in 3048 species: Archae - 709; Bacteria - 24685; Metazoa - 865; Fungi - 647; Plants - 730; Viruses - 0; Other Eukaryotes - 3917 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL09-54-N06 introduced

  • F08303 - Specific phenotypes were NOT observed.
  • F11910 - Specific phenotypes were NOT observed.
  • F17725 - seed::increased size
  • F23322 - Specific phenotypes were NOT observed.
  • F28935 - seed::increased size
  • F29028 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

14 mutant lines found