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RAFL11-01-E14

RIKEN Arabidopsis Full-length cDNAs

Resource: (pda10048)

full reading [+] show sequence

Nucleotide sequence: RAFL11-01-E14 - full reading 
GGGAGGCACA CATTACACAC CAAAGCAGTG TTTTCTTTCT CCTGAAAAGA 
TTTTTCAAGC TCTCTCTTTC TCTCAAAGGT AAATGCTTCT CTGATTTCGC 
CATTTTTGCT TACTCGATTC AAAAGACATG TCGCCGGCAG CTACGGAGAC 
GGAGCGATGG TGCGTTGTTA CCGGCGGTAG AGGATTCGCG GCGAGGCATC 
TTGTTGAAAT GCTCGTACGC TACGAAATGT TTTGCGTCCG TATCGCCGAT 
TTAGCTCCGG CGATAATGCT TGATCCTCAG GAAGGGAACG GAGTTCTCGA 
CGAAGGATTA AGATCTGGTC GTGTTCAATA TATCTCCGCT GATCTTCGAG 
ATAAATCTCA AGTCGTTAAA GCTTTTCAGG GAGCGGAGGT GGTGTTTCAT 
ATGGCAGCTC CAGATTCTTC TATTAACAAT CATCAGCTTC AGTATTCGGT 
GAATGTTCAA GGGACACAAA ATGTGATTGA TGCTTGTGTT GATGTTGGAG 
TAAAGAGGCT TATTTACACT AGCTCTCCTA GTGTTGTGTT TGATGGTGTT 
CATGGTATTT TGAACGGTAC TGAATCAATG GCTTACCCAA TTAAGCACAA 
TGACTCATAT TCAGCAACTA AAGCTGAAGG AGAAGAGTTG ATCATGAAGG 
CTAATGGTAG AAATGGACTA CTCACTTGTT GCATACGTCC AAGCAGTATT 
TTTGGTCCCG GTGATAGATT ATTGGTTCCG TCGCTTGTTG CTGCTGCTAG 
GGCTGGGAAA TCTAAGCTTC AGGAGGAGAT GTTTCTACAA AAGCTGCAGG 
ACAGTGGATT TACAGGCATA TTTCATTACC AACATGGAGC CAATTAAATT 
TTGGGAATTT ATGTCACAGC TCCTTGATGG ACTTGGTTAC GAGAGGCCAA 
GCATAAAGAT CCCTGCGTTT ATCATGATGC CGATTGCGCA TCTGGTTGAA 
CTGACATATA AAGTGTTGGG ACCATATGGG ATGACAGTAC CACAACTAAC 
ACCTTCAAGA GTTAGGCTTC TCTCATGCAG CAGAACTTTC GATTCCACGA 
AAGCAAAGGA TCGACTAGGC TATGCTCCTG TGGTCCCACT TCAGGAAGGT 
ATACGGAGGA CGATAGACTC ATTCTCTCAC CTAACAGCTG GAAGTCAATC 
CAAAAGAGAG GGCCCATCCA AGGCTTCTAG GATTCTTGGA GGCGGAAAGG 
TTGCTGATAC ACTTCTCTGG AAGGATTTAA AGCAGACTCT TATTGCCATA 
TTCATTCTAA TTAGTATCTA TTATAACTTT GTTGCAACTG GGTCTACCGT 
CGTAACTGCT CTTTCGAAGG CTCTATTGGT AGCATCTGTA TTTTTGTTCC 
TCCATGGCAT TCTACCAGAG AAAATATTTG GTTATACAGT TGAAAAGATT 
CCTGCATCGC AATTTCACCT CTCAAAAGAC TCATCACATG ACCTCTCATT 
GTCAGTGATC TCTTCATGGA ACACCACTGT AAAAGCTTTA AGATCTCTTT 
GCCAGGGGAA TGATTGGAGC TTCTTTTTCA AGGTGGTCTT CGTTTTGTTA 
GCATTGAGTT TGGCTGGAGC CATATCTCTT CACAGCATAT TTGTTATAGG 
ACTGCCCATT GCCTTCCTTG CTTTTCTCGT GTACGAAAAG AAAGAGCAAG 
AGATCGACTC AATAGTGGTG AGTTTTAAAT CTTTCGCATG CAAGCACAAA 
TCCGATGTGT ATGAGAAATT GTTCGGAAGC AAGAAACATG ACTAAAAACC 
CTTTTTTTGC CCAGCATTTG TAATTTTCTT TTGTTTCCAA CGACCTCTAA 
CTCTGGTTTG AGCTTTTTTC AAGTTTTATT ATTTGTGAAC

Gene models with high sequence identity

  1. AT2G26260.2 [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
    E-value: 0; Score: 98.96

 

InterPro Scan Digest

Program Description E-value
HMMPanther 3 BETA-HYDROXYSTEROID DEHYDROGENASE 2.6e-35
HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 2.6e-35
HMMPanther 3 BETA-HYDROXYSTEROID DEHYDROGENASE 4e-86
HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 4e-86
superfamily NAD(P)-binding domain 1.9e-40
superfamily NAD(P)-binding domain 3.9e-11
ProfileScan Reticulon 14.371
HMMPfam 3-beta hydroxysteroid dehydrogenase/isomerase 3.7e-55
HMMPfam Reticulon 3.3e-31
HMMPfam 3-beta hydroxysteroid dehydrogenase/isomerase 0.00012
Gene3D NAD(P)-binding domain 5.2e-34

See the detailed result >

AT2G26260.2

Model type
Protein coding
Short Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Curator Summary
Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Computational Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (3BETAHSD/D2); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Reticulon (InterPro:IPR003388), 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (TAIR:AT1G47290.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL11-01-E14 introduced

  • F04423 - Specific phenotypes were NOT observed.
  • F19129 - seed::increased size / seed::increased length
  • F24906 - rosette leaf::abnormal

Mutant lines with disrupted or induced

4 mutant lines found