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RAFL11-12-F09

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdx27069)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL11-12-F09 - 5 prime EST 
GGAGAGCTAA ATCATTTACG TTTTGATCGA CAAAAATGGA GTTGATAAAC 
GACTTTCTCA ACCTAACTGC TCCTTTCTTC ACCTTCTTTG GTCTCTGCTT 
CTTCTTGCCT CCTTTTTACT TCTTCAAGTT CTTGCAGTCT ATTTTCTCGA 
CAATTTTCTC TGAAAATCTT TACGGGAAAG TTGTTCTCAT CACTGGTGCT 
TCCTCCGGTA TAGGCGAGCA ATTGGCATAT GAGTACGCAT GTAGAGGTGC 
ATGTTTAGCC CTGACCGCCC GAAGGAAGAA CCGTCTAGAG GAAGTGGCAG 
AGATTGCTCG TGAACTCGGA TCTCCCAATG TTGTTACCGT TCATGCTGAT 
GTCTCCAAAC CTGATGACTG TAGACGAATC GTTGATGACA CCATCACCCA 
TTTTGGCAGA TTGGATCATC TTGTAAATAA TGCTGGGATG ACGCAGATTT 
CAATGTTTGA GAACATTGAA GATATAACGA GGACAAAAGC AGTTTTGGAT 
ACTAACTTCT GGGGATCGGT TNATACCACT CGTGCTGCGC TTNCATACCN 
TTAGACAAAG TAATGGTAAG ATTGTGGCTA TGTC

Gene models with high sequence identity

  1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
    E-value: 0; Score: 99.49

3 prime EST [+] show sequence

Nucleotide sequence: RAFL11-12-F09 - 3 prime EST 
AAAGACACAT AGTTAATATA CATAGTCAAA AGTACCATTA ACAAAAACAA 
ACCTACATTT TCACCCATTT CTTTGATAAT TATTACTCAT TGCACATACT 
CATGATACAT CTCATCCATT TATTATGTAT TGACCCCTCT CTAATCCGAC 
TTGATTTCTG GAGTTCTGAT AGATTCCGGG TACAGAGTAC TACGTACACC 
AGGAATGTCC ATGATCCTCT TGTTGAGTGG AGTATCCTCG GACATACCAG 
TTCCAGTCAT GTTCAGTAAC CGACAACCCC ACTCAATCAA CTCCGGAAAT 
AGCACTTTCC AAAGGTACGT CACCTTAAAC CATGATGGCT CTGTCAAGTA 
TTTTTGTTTC CGGGCACACA CCGTTCACTA TGGACTTGGC ACATCCTGAT 
CCTGACGCTG

Gene models with high sequence identity

  1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
    E-value: 0; Score: 98.04

AT5G50700.1

Model type
Protein coding
Short Description
hydroxysteroid dehydrogenase 1
Curator Summary
Encodes a hydroxysteroid dehydrogenase HSD1. Two copies of HSD1 (At5g50600 and At5g50700) exist in the Arabidopsis genome as a result of an exact 33-kb duplication on chromosome 5 encompassing seven genes. There are five homologs of HSD1 in Arabidopsis (HSD2-At3g47350, HSD3-At3g47360, HSD4-At5g50590, HSD4-At5g50690 and HSD6-At5g50770; HSD4 has two copies due to the same gene duplication event occurred to HSD1) (Plant Cell Physiology 50:1463). At5g50690 is also named as HSD7 (Plant Physiology 145:87). HSD1 is identified from the proteome of oil bodies from mature seeds. Transcription of HSD1 is specifically and highly induced in oil-accumulating tissues of mature seeds; transcript disappears during germination.
Computational Description
hydroxysteroid dehydrogenase 1 (HSD1); FUNCTIONS IN: oxidoreductase activity, binding, catalytic activity; INVOLVED IN: oxidation reduction, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: seed; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: hydroxysteroid dehydrogenase 1 (TAIR:AT5G50600.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL11-12-F09 introduced

  • F03207 - seed::increased length
  • F07528 - Specific phenotypes were NOT observed.
  • F15327 - Specific phenotypes were NOT observed.
  • F16504 - Specific phenotypes were NOT observed.
  • F21543 - seed::increased size / seed::increased length
  • F23044 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

12 mutant lines found