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RAFL14-14-L14

RIKEN Arabidopsis Full-length cDNAs

Resource: (pdz88429)

5 prime EST [+] show sequence

Nucleotide sequence: RAFL14-14-L14 - 5 prime EST 
AACCGATGTA TTCTTCTCCT TGTCCTTCCT TACTCTCTAT ATTTTTCTCA 
ATTCTCCGAT TTGATCCAAC GATTTCACAG ATCTCTGATC TAAATGGCAT 
CCAACGGTGA TAAAGGACTG ATCGTTAGCT TCGGCGAGAT GCTAATCGAT 
TTCGTCCCTA CCGAATCAGG CGTCTCTCTC GCCGAAGCTC CTGGATTCCT 
CAAAGCTCCA GGCGGTGCTC CCGCTAACGT CGCAATCGCT GTTTCTCGTC 
TCGGTGGACG ATCTGCTTTC GTCGGAAAAC TTGGAGACGA CGAGTTCGGT 
CATATGTTGG CTGGGATTTT GAGGAAGAAC GGTGTTGATG ATCAGGGGAT 
TAACTTCGAT ACTGGAGCTA GAACTGCTTT AGCTTTCGTT ACGTTACGTG 
CTGATGGAGA TCGTGAGTTT ATGTTTTACC GCAATCCTAG CGCCGATATG 
CTTCTTCGTC CTGATGAACT CAATCTCGAC CTCATCAGAT CCGCGAAAGT 
GTTTCACTAT GGATCAATAA GTTTGATAGT GGAGCCGNGT AGGTCAGCTC 
ATTTGAGGCC ATGGAAGTGG CGAAAGAAGC ANGAGCNCTT

Gene models with high sequence identity

  1. AT2G31390.1 [+] show detail - pfkB-like carbohydrate kinase family protein
    E-value: 0; Score: 99.32

3 prime EST [+] show sequence

Nucleotide sequence: RAFL14-14-L14 - 3 prime EST 
CCATAATTTA TTTTTATTAC TTCAATCTTA AAACATCTTA CACAAACACC 
ACATACTGTA TATATGAGCA ACAAATAAAT GATAAAAATA AATAAAAACT 
TGATTGAGTT ACAAAGGCAA TGCCAAGTAA CATATTCATG TATCAAAGAA 
TCAAGAATTT AAGTGCACTC GGCAGGAACT CCTCGAGAAC CGTGGACGTT 
GTTTCCTGTC GAAGTCGCAG ACTCGTCATC ACAGCTCAGG CGAGTAATTC 
TGAGTTTCTT CTATCTACGC GCAATATCTC TCAAAGGAGG CCTCTTCAAA 
GGTACCACAA TCATATCCTC TCGTTTCTAT CTGAAGAATC AGAGAGAAGA 
GATATATAGA GGATGCCTGT GATCTCCAAA GCTTCATCTA CCACCACCAA 
TTCATCGATT CCTTCGTGTT CTAGGATTGG AGG

Gene models with high sequence identity

  1. AT2G43430.1 [+] show detail - glyoxalase 2-1
    E-value: 5e-133; Score: 100.00

AT2G31390.1

Model type
Protein coding
Short Description
pfkB-like carbohydrate kinase family protein
Computational Description
pfkB-like carbohydrate kinase family protein; FUNCTIONS IN: kinase activity, ribokinase activity; INVOLVED IN: D-ribose metabolic process, acetate fermentation, sucrose biosynthetic process, sucrose catabolic process, using beta-fructofuranosidase; LOCATED IN: plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Carbohydrate/purine kinase (InterPro:IPR011611), Ribokinase (InterPro:IPR002139), Carbohydrate/puine kinase, PfkB, conserved site (InterPro:IPR002173); BEST Arabidopsis thaliana protein match is: pfkB-like carbohydrate kinase family protein (TAIR:AT1G06030.1); Has 18509 Blast hits to 18503 proteins in 2397 species: Archae - 386; Bacteria - 14053; Metazoa - 143; Fungi - 116; Plants - 466; Viruses - 0; Other Eukaryotes - 3345 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G43430.1

Model type
Protein coding
Short Description
glyoxalase 2-1
Curator Summary
Encodes a predicted mitochondrial glyoxalase GLX2-1. Studies using recombinant protein show that GLX2-1 contains a dinuclear metal binding site, but does not have glyoxalase 2 activity.
Computational Description
glyoxalase 2-1 (GLX2-1); CONTAINS InterPro DOMAIN/s: Beta-lactamase, class B, conserved site (InterPro:IPR001018), Beta-lactamase-like (InterPro:IPR001279), Hydroxyacylglutathione hydrolase (InterPro:IPR017782); BEST Arabidopsis thaliana protein match is: glyoxalase 2-4 (TAIR:AT1G06130.2); Has 15540 Blast hits to 15539 proteins in 2481 species: Archae - 339; Bacteria - 10160; Metazoa - 480; Fungi - 326; Plants - 230; Viruses - 0; Other Eukaryotes - 4005 (source: NCBI BLink).
Link
InterPro Scan - TAIR

Mutant lines with RAFL14-14-L14 introduced

  • F06925 - Specific phenotypes were NOT observed.
  • F10302 - seed::increased size / seed::increased length
  • F11912 - rosette leaf::dark green / rosette leaf::abnormal / rosette leaf::increased width / stem::abnormal / cauline leaf::epinastic / cauline leaf::abnormal / sporophyte vegetative stage::decreased speed / rosette leaf::increased width / rosette leaf::present in greater numbers in organism / rosette leaf::abnormal / cauline leaf::increased size / cauline leaf::abnormal
  • F28624 - Specific phenotypes were NOT observed.

Mutant lines with disrupted or induced

17 mutant lines found

5 mutant lines found