2,090 proteins with a targeting signal to chloroplasts were identified by at least 3 of the 4 different prediction programs (Richly and Leister, 2004).
Among the 2,495 tagged lines, we collected the homozygous lines that did not exhibit visible phenotypes. This collection has the potential to be a powerful tool for the screening of chloroplast mutants with biochemical or conditional phenotypes
We have listed the primer pairs used for amplifying DNA fragments of approximately 1 kb from genomic DNA; these primers flank the predicted T-DNA insertion loci and facilitate the detection of T-DNA insertion in each gene. Over 1,300 primer pairs for WISC and SALK T-DNA-tagged lines have been designed to date.