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11-0149-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 1668320 [Map]; Strand: c
      • AT1G05580.2 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon2 (CDS)
      • AT1G05580.1 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 11-0149-1 - 5-prime edge
    CCTACAGATT CACAATGGTT CTTGAGACAT TCGCTAACTT AGCTCTCGTC 
    TACAACATCT TCCTCCTTGG TCTAGGGATG GACCTGAGGA TGGTGCGGAT
    CACCGAGCTC AAACCGGTCA TCATTGCTTT TACAGGGCTT CTTGTTGCTT
    TACCGGTTGG TGCCTTTCTT TACTATCTCC CGGGAAATGG CCATCCAGAC
    AAGATCATCT CTGGTTGCGT CTTCTGGTCT GTCGCGCTAG CCTGTACCAA
    CTTCCCTGAC CTTGCAAGGA TC
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 1668439 [Map]; Strand: c
      • AT1G05580.2 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon2 (5UTR)
      • AT1G05580.1 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon2 (CDS)
      • AT1G05590.1 - [+] show detail - beta-hexosaminidase 2
        Distance: -911; Region: promoter
    Flanking Genome Sequence: 11-0149-1 - 3-prime edge
    TTAGGGGAAT ACATGTGCGA TTATGAAACG TGTGTTCCCG AGCACACTTG 
    GGCTAAATAA TAATCCACAC TGCAAAAATA CAATCACAAC AAATCAAGAC
    ACATACGGAA CTAGAGAGCT AAAAAATTGA TGTAGCAAAA ATTGCATACN
    GTACGAGGAT CTGAGCTACG AAAGGAGGAA GATACAAGGG TCGGGTTAGA
    TAGTAGAGGA CACGATAGCT AAGGTTCGCG ACAAAGAGTT GCGTTATGAA
    GAACGGAAGG GAAGAGGCTA GCGTACTTCC GCTCTCCCAT CCCTTTTGTT
    CTCTCTTCTC AAAGAGAAGA CTTTGGTCAT AACACACAAT CTTTCCAAAA
    CGCGATTCTT CAATATCATA GTTAGGATTT GTCACATTAA GGGGGGCTCC
    GGAAGACATC TCCTCCTACG ATGGCTGGTC GGAATCCCCA AAAAAAATGA
    CAATACTATC TGCCTGAACC CAACGTTAGT TTCANACNAA ACATTGGGTT
    CATATAGACT GCAATAACAA CTAATTTGCA ACCTCTTCCA AGTTTTAGTT
    CAGACGAGAT GGCTAATTAT AGATGATGTA TTAGTTGTCA TTGGGGAATT

AT1G05580.1

Model type
Protein coding
Short Description
cation/H+ exchanger 23
Curator Summary
member of Putative Na+/H+ antiporter family
Computational Description
cation/H+ exchanger 23 (CHX23); FUNCTIONS IN: monovalent cation:hydrogen antiporter activity, sodium:hydrogen antiporter activity; INVOLVED IN: cation transport; LOCATED IN: integral to membrane; EXPRESSED IN: male gametophyte, leaf; CONTAINS InterPro DOMAIN/s: Cation/H+ exchanger (InterPro:IPR006153); BEST Arabidopsis thaliana protein match is: cation/H+ exchanger 21 (TAIR:AT2G31910.1); Has 6204 Blast hits to 6178 proteins in 1654 species: Archae - 257; Bacteria - 4841; Metazoa - 0; Fungi - 132; Plants - 575; Viruses - 0; Other Eukaryotes - 399 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G05580.2

Model type
Protein coding
Short Description
cation/H+ exchanger 23
Curator Summary
member of Putative Na+/H+ antiporter family
Computational Description
cation/H+ exchanger 23 (CHX23); FUNCTIONS IN: monovalent cation:hydrogen antiporter activity, sodium:hydrogen antiporter activity; INVOLVED IN: cation transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: male gametophyte, leaf; CONTAINS InterPro DOMAIN/s: Cation/H+ exchanger (InterPro:IPR006153); BEST Arabidopsis thaliana protein match is: cation/H+ exchanger 21 (TAIR:AT2G31910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G05590.1

Model type
Protein coding
Short Description
beta-hexosaminidase 2
Curator Summary
Encodes a protein with β-hexosaminidase activity (the enzyme is active with p-nitrophenyl-β-N-acetylglucosaminide as substrate but displayed only a minor activity toward p-nitrophenyl-β-N-acetylgalactosaminide). Chitotriose-PA was digested almost completely overnight by a 50% ammonium sulfate fraction of a supernatant yeast expressing AtHEX3.
Computational Description
beta-hexosaminidase 2 (HEXO2); FUNCTIONS IN: UDP-glucosyltransferase activity, hexosaminidase activity, beta-N-acetylhexosaminidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 20, catalytic core (InterPro:IPR015883), Beta-N-acetylhexosaminidase, subunit a/b (InterPro:IPR015882), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, family 20 (InterPro:IPR001540), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta-hexosaminidase 1 (TAIR:AT3G55260.1); Has 3806 Blast hits to 3725 proteins in 709 species: Archae - 2; Bacteria - 2403; Metazoa - 477; Fungi - 234; Plants - 128; Viruses - 0; Other Eukaryotes - 562 (source: NCBI BLink).
Link
InterPro Scan - TAIR