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11-0239-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 8710680 [Map]; Strand: w
      • AT2G20180.3 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: -3780; Region: intergenic
      • AT2G20190.1 - [+] show detail - CLIP-associated protein
        Distance: 916; Region: intergenic
    Flanking Genome Sequence: 11-0239-1 - 5-prime edge
    CATTTGCCCA CACCTTCTAT TTCTAGTACT ATTTTATTTG TATATCAAAC 
    GAGAAAATAA AAACAATACG ATTCTAAATT TATCTCACGA CTAGCACATG
    ATTTGAGGC

AT2G20180.3

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1).
Link
InterPro Scan - TAIR

AT2G20190.1

Model type
Protein coding
Short Description
CLIP-associated protein
Curator Summary
Encodes a microtubule-associated protein that is involved in both cell division and cell expansion. It likely promotes microtubule stability.
Computational Description
CLIP-associated protein (CLASP); FUNCTIONS IN: binding; INVOLVED IN: mitosis, protein stabilization, cell growth; LOCATED IN: spindle microtubule, plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: HEAT (InterPro:IPR000357), Armadillo-like helical (InterPro:IPR011989), HEAT, type 2 (InterPro:IPR021133), Armadillo-type fold (InterPro:IPR016024); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR