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Mutant Lines
11-0282-1

11-0282-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 5; Position: 1716654 [Map]; Strand: w
- AT5G05700.1 - [+] show detail - arginine-tRNA protein transferase 1
  Distance: -923; Region: promoter
- AT5G05720.1 - [+] show detail - RNA-binding (RRM/RBD/RNP motifs) family protein
  Distance: -217; Region: promoter

CATGTACTTA CCAAATATGG TAACAGAAGC AATACCTTTT GCACGTGATC 
AAATACAATA ACTTCGTCGT ACAGACTAAG ATGCATGTAT GGAAAGTTCC 
TCTCGTCATT AAGTGTCCTA AACCCTACCC GCTTCCATTC AGGGCCGGGT 
CTGGAAGGAA CAACAAAATA CCTAAAACCC CTCTCCTCTT CTTCTTCTTC 
CTAGTCCGAC ACTCTTAGAG TCTTCAAAAA CATTGCAAGA AGGAAACAAA 
ATCCATCTTC TTCGTCTATT AGAAACCCTA ACTTGAGAAT GTAAGTATTG 
ATTTCCCACA ACAGTTTTCT TTTTCCTTTA ACTAATTAGG TTTAGCAGAA 
AACAATCAAT GTAGGCAAGT TATTTTTTTT TTTNGGNCCT NGNGNAAATA 
ACNGGNNTGG GANAANNGTA AAAAATTTNC NTTCCAAANN TGGNANAAAN 
NANCNATTTC AAAANGTTTT TNCCCAAAAG GGNNAANNCC CGGCCGNNAA 
NTNGANNCGG NAAAGGGNCC TTNCAANNNN GNGGGTTTTN NNNNGTTAAT 
TNTNGGGTTT NNGNGGAAAN CNCTNNTNTT TNNNTTTGGC TTTTNCNGGN 
NNGGCAAANA NTNAAANANG GGCTNTNTTG GGNTTNTNGN NCNGANAAAA 
AACCTNANNA ANCCTTCNCC NTTNNGTAAA CAAGGGGN

3 prime edge [+] show sequence
1. Chromosome: 5; Position: 1716538 [Map]; Strand: w
  - AT5G05700.1 - [+] show detail - arginine-tRNA protein transferase 1
    Distance: -807; Region: promoter
  - AT5G05720.1 - [+] show detail - RNA-binding (RRM/RBD/RNP motifs) family protein
    Distance: -333; Region: promoter
Flanking Genome Sequence: 11-0282-1 - 3-prime edge
```
AGTACATGTT TGAAGAACCA TGTAAGTCTT TGAACCAGAA GCGTTGCCCT 
TGAAGAATTA GGTTTTATAT TATATGTTTT TCGGATATGA GTTCATGTTT 
TCAAGCAGAG ACAAAGCT
```

AT5G05700.1

Model type: Protein coding
Short Description: arginine-tRNA protein transferase 1
Curator Summary: Encodes an arginyl-tRNA:protein transferase (ATE1), a component of the N-end rule pathway that targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Arabidopsis contains two ATE genes: At5g05700/ATE1, At3g11240/ATE2. Another component of the N-end rule pathway is At5g02310/PROTEOLYSIS6 (PRT6). PRT6 and ATE were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. Mutants of ATE1 also display delayed leaf senescence.
Computational Description: arginine-tRNA protein transferase 1 (ATE1); CONTAINS InterPro DOMAIN/s: Arginine-tRNA-protein transferase, N-terminal (InterPro:IPR007471), Arginine-tRNA-protein transferase 1, eukaryotic (InterPro:IPR017137), Arginine-tRNA-protein transferase, C-terminal (InterPro:IPR007472); BEST Arabidopsis thaliana protein match is: arginine-tRNA protein transferase 2 (TAIR:AT3G11240.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G05720.1

Model type: Protein coding
Short Description: RNA-binding (RRM/RBD/RNP motifs) family protein
Computational Description: RNA-binding (RRM/RBD/RNP motifs) family protein; FUNCTIONS IN: RNA binding, nucleotide binding, nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: nucleotide binding;nucleic acid binding;RNA binding (TAIR:AT4G19610.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR