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11-0841-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage		 Phenotype
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Flowering & Growth::dwarf/small)

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6589237 [Map]; Strand: c
      • AT5G19530.1 - [+] show detail - S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
        Distance: 0; Region: exon9 (CDS)
      • AT5G19530.2 - [+] show detail - S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
        Distance: 0; Region: exon10 (CDS)
    Flanking Genome Sequence: 11-0841-1 - 5-prime edge 
    ATAAACTAAC AAAATCGGTT ATACTGATAA CGGTCGGTAC GGGATTTTCC 
    CATCCTACTT TGTCACTGAA GTTTATAGTG AAGAGAATGC GAGATTCATT 
    CATGGTCATG GTGTGGCGTA CCGGCATATT TAAAGACGAA CCGGTTTCAG 
    TTTCAGTGTT ATTACCAAAC CCATGTCACA AAAACAAAAG GCCGGTTTCT 
    TTTCTCCGCA CAGAACCGGG TGTTGTCTTG AATCTTGATT ACTTTGGTTC 
    GGTTTTATTT TCTACATTGC TTTTCGTTTT CTTGTTCTTC CCTCAAGTTA 
    TTCCGGTTTA ACAAGACTAT ATTGCTTACT AAGAAAGACC GATCTTCAAT 
    TACTTATTTC TCCACACCAA ATCTAGAAAT CGAAATAGGC CAATTATGGT 
    TCAATTGAAG TAAACCGAGT TTCTGAGTGC AATTAAACCG AAACAAATCA 
    AATTTAAACC GAAGCAAATG AAGGAAACTA GTACTAGTGG AATAAAATGC 
    AAAAGAAGAG AAGGAAAACA CGATCATTTG TGACCAGTGA GATTGACATC 
    TTGAGGAAGG AGAGTGTAAT CGATCTCAAG ATCCTCGAGA ATT

AT5G19530.1

Model type
Protein coding
Short Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Curator Summary
Encodes a spermine synthase. Required for internode elongation and vascular development, specifically in the mechanism that defines the boundaries between veins and nonvein regions. This mechanism may be mediated by polar auxin transport. Though ACL5 has been shown to function as a spermine synthase in E. coli, an ACL5 knockout has no effect on the endogenous levels of free and conjugated polyamines in Arabidopsis, suggesting that ACL5 may have a very specific or altogether different in vivo function.
Computational Description
ACAULIS 5 (ACL5); CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: spermidine synthase 1 (TAIR:AT1G23820.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G19530.2

Model type
Protein coding
Short Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Curator Summary
Encodes a spermine synthase. Required for internode elongation and vascular development, specifically in the mechanism that defines the boundaries between veins and nonvein regions. This mechanism may be mediated by polar auxin transport. Though ACL5 has been shown to function as a spermine synthase in E. coli, an ACL5 knockout has no effect on the endogenous levels of free and conjugated polyamines in Arabidopsis, suggesting that ACL5 may have a very specific or altogether different in vivo function.
Computational Description
ACAULIS 5 (ACL5); CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: spermidine synthase 1 (TAIR:AT1G23820.1); Has 4823 Blast hits to 4823 proteins in 1442 species: Archae - 170; Bacteria - 2608; Metazoa - 258; Fungi - 194; Plants - 443; Viruses - 0; Other Eukaryotes - 1150 (source: NCBI BLink).
Link
InterPro Scan - TAIR