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11-1154-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 8705628 [Map]; Strand: w
      • AT2G20180.1 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: intron3 (CDS)
      • AT2G20180.2 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: intron2 (CDS)
      • AT2G20180.3 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: intron3 (CDS)
    Flanking Genome Sequence: 11-1154-1 - 5-prime edge
    CTGAAAACTA CAAAGTCAGA CTAAAATGCG CCGTATTTGA CTTGCGCACG 
    ATAGCTAACA ACGACTCTGT TCTGTGTTTT TTTGACTCAC CTCGCTACGG
    GATTCAGTTT CATCAGTAGT GGTGGCTTCT CTCTCTTTTC GTTTCCTATC
    ATCGACGTTC GTCTTCTCCG GTTCGATTTC GCTCTTTGAC ACTACAGATG
    ACGATGTACC GGTTATCTCG ATCGCCGGCG CCGTCATAGC CACTGCTTTT
    CCCTTCCGAT TATACGCGCC GCCTCCAGCT ACGGCGGAAC TGTCAGTACC
    ATCCGTCCGC CGTGTTAAAC CGGATTCACT CGCCGCCGCC GACGGTGTTG
    CGCTAGGACT TACCTGCGTA GATTCTCTCA CAACCGCCTT CGAAAGCAAC
    GGTCCAGATT CACCACCTCT ACCGTTATTA AAATCCCCTC TCAGCCTCGA
    GAAATTCATG AAGTTCCTCA CCGGCGGCCT CGACTCATTC GTCGAAGGTA
    CTGGCGGTCT CGCGGCGGTG ACTTGACTCA CCGTCGCGGT AGCCGTCGCA
    GTAGGTGCGG CGGAGAANAG AAGATCTGAG CAGAAATCAT CGTCACGGAG
    AGGATAATGA AGCCAAGAAG TCATTTCATC TTCTTGAATA AAAAGATTCT
    GATCTGAAAA AAGTTGGCTG CTGAGGATCA CCANCCCG
  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 8705512 [Map]; Strand: w
      • AT2G20180.1 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon4 (CDS)
      • AT2G20180.2 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon3 (CDS)
      • AT2G20180.3 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon4 (CDS)
    Flanking Genome Sequence: 11-1154-1 - 3-prime edge
    GTTTTCAGCG CATTTTTAAT CAACTTTTTT GGTGTTTTCT TGTGGTGCAG 
    GAAACAAAAC AAGCACGTGT ATCAACAACA TCTACCAAGA GATCTCGTGC
    TGCTGAAGTT CATAATCTCT CTGAAAGAGT ACNGTTTGTG TTTTTTCTTA
    TGTTTTTGTA ATCTTTTTCG ATAAAGATTT CGCAATGAAT CTGAAGAATG
    TCGCGGATTT GGTAACAGAA ACGGAGAGAT AGGATCAATG AGAGAATGAA
    AGCT

AT2G20180.1

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1); Has 4635 Blast hits to 4615 proteins in 348 species: Archae - 0; Bacteria - 26; Metazoa - 878; Fungi - 251; Plants - 3376; Viruses - 2; Other Eukaryotes - 102 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G20180.2

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1); Has 4736 Blast hits to 4693 proteins in 351 species: Archae - 0; Bacteria - 27; Metazoa - 921; Fungi - 265; Plants - 3401; Viruses - 2; Other Eukaryotes - 120 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G20180.3

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1).
Link
InterPro Scan - TAIR