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Mutant Lines
11-2385-1

11-2385-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity /structure development stage	Phenotype
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997) (Seed yield::sterile)
stem (PO:0009047)	high saturation (PATO:0001229) (Stems::waxy)

Anatomical entity
/structure development stage

Phenotype

seed (PO:0009010)

present in fewer numbers in organism (PATO:0001997)
(Seed yield::sterile)

stem (PO:0009047)

high saturation (PATO:0001229)
(Stems::waxy)

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 1; Position: 418926 [Map]; Strand: c
- AT1G02205.3 - [+] show detail - Fatty acid hydroxylase superfamily
  Distance: 0; Region: intron1 (CDS)
- AT1G02205.1 - [+] show detail - Fatty acid hydroxylase superfamily
  Distance: 0; Region: intron1 (5UTR)
- AT1G02205.2 - [+] show detail - Fatty acid hydroxylase superfamily
  Distance: 0; Region: intron1 (CDS)

ATTACGATGT ACTTTTGTAT TGTTTCAATG TCAAGAAAAA CATATATATC 
AGTAACGTAA CGGGTTTCGA TCATAAGCAC GCGTTTTTTT AAATTTAAAG 
GACAAGAAAA CAGAGTGCAC CTTGAAACTT CCGAGGGGTG TCCAAGGCCA 
ATCGGTGAGG ACTCCTGGTT TTGTGGCCAT TATACCGTCG AATGTAATAT 
GTTTGTGTCG TCACTGTGTC TCTTCAACTC TTGTGAGCCA AGGTACATTA 
TATAGCAAGG TTTATATTAA ACTCCTTAAT TGGTAGATAT GTGGAATGAC 
GTAACCACCC CTATCTTCCC ACCAGTCCAA TTCCTTGTGG TGGGGCCAGC 
TAAGACCAAA CATGTACTAC TACCATTCGA TGCTCTCCAC ATGGATC

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 419042 [Map]; Strand: c
  - AT1G02205.3 - [+] show detail - Fatty acid hydroxylase superfamily
    Distance: 0; Region: exon2 (CDS)
  - AT1G02205.1 - [+] show detail - Fatty acid hydroxylase superfamily
    Distance: 0; Region: exon2 (5UTR)
  - AT1G02205.2 - [+] show detail - Fatty acid hydroxylase superfamily
    Distance: 0; Region: exon2 (CDS)
Flanking Genome Sequence: 11-2385-1 - 3-prime edge
```
ATCGTAATAG CACCATGGGC TGTCCATAGC ACATACAGGT TTGTGACAGA 
TGATCCAGAG AAGAGGGATC TCGGGTACTT CCTTGTGTTC CCCTTCTTGC 
TCTTCAGAAT T
```

AT1G02205.1

Model type: Protein coding
Short Description: Fatty acid hydroxylase superfamily
Curator Summary: Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
Computational Description: ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endomembrane system, endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 341 Blast hits to 339 proteins in 49 species: Archae - 0; Bacteria - 44; Metazoa - 0; Fungi - 0; Plants - 284; Viruses - 0; Other Eukaryotes - 13 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G02205.2

Model type: Protein coding
Short Description: Fatty acid hydroxylase superfamily
Curator Summary: Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
Computational Description: ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G02205.3

Model type: Protein coding
Short Description: Fatty acid hydroxylase superfamily
Curator Summary: Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
Computational Description: ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1).
Link: InterPro Scan - TAIR