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11-5092-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage
Phenotype
seed (PO:0009010)
  • abnormal (PATO:0000460)
    (Seed yield::seed shape)

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 544925 [Map]; Strand: c
      • AT1G02580.1 - [+] show detail - SET domain-containing protein
        Distance: 0; Region: intron1 (CDS)
    Flanking Genome Sequence: 11-5092-1 - 5-prime edge
    CTCACCATCG TCCTCATGGT TTTCCTAGAC AAATTCAAAA ATAAAAGTTT 
    TATGGATCAT GCATGGGACG AATAACATAA TAGATCACAA GAAAATTCAA
    CCCTAAATGT TATTGATCTA TCATAAACAC ACAATTAAAA TATATGGATT
    TCATAACCTA GTCAATTCAT ATATGTTTGG AGTAAAACTA ACCTTCTCCA
    TTAACCACTC GCCTCTTCTT TTTTTTCTCG TCTTCTCTGA TGTTGGTGAA
    AAGGATAATG CAAAGGGTTT TTTAAATAAN AAGTTGATTT TTTTTTGTAA
    AAAACCTTAT TAAGGAAAAT TATAATCTGG AAAAAACGTA TTTGGACTTG
    CTNGCTTTAA AAGGAAAATA ATATGGGAAA TTTAAAAAAA TTTTAATTTT
    CAAAATATAG TACTATATGT ATGTAAATGT NGGGTTTGCT TGACGTTTGA
    CAGCTCAAGT TTAAATTCNT NTNCAATNTT ATAAAAAAAA AAAGGGGGTN
    ACCNNNTTTT TTNGGNANNC CTGGGTTNAN NAANCNAANN AAAAANGGNG
    GAAAAAAAAA NNNNANNTTT NGGNNGGGNC NGGGAAAACT NTTTTAAGGG
    NNNTTTTTTT TTTNNNNNTN CNTCCCAANC NNNTTTTNTT TTNAANNNNN
    NNNCCGGGGC GNNAACNNCC GGNNCCCNNN A
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 544999 [Map]; Strand: c
      • AT1G02580.1 - [+] show detail - SET domain-containing protein
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 11-5092-1 - 3-prime edge
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCG 
    GGTTTGCCAC CCGAACTAAA TCAGATAAAA GAGCAAATCG AAAAGGAGAG
    ATTTCTGCAT ATCAAGGTAA GAGACATTTG GTTGCTTTAA TATTTTATTC
    TCTTCTGTAT GTTTTTCTGA AAATTAAGGA GAGGAGAGGA CNTTAATCTC
    ATAACTATAC GATTCCAAAG AGATGTTAAG ATACATCTAG TAAACAGTTA
    TACATTAGTC ATAATCTTTA AAACTAAAAA GAGAAATTTC CAAACTTTTA
    AATTAAAAAC AGAATTTAGA AAATGCCAGC GAATCGATAA CGACATCCAG
    ATCTGTCGGG TATCCAAAAC TTAGAATAAA AAAATAATTA ATATATTTAT
    AATATAAAGC TGGAACTTAG GTTATAAAAT AAAATTGAAA ATAATAGTAG
    ATTTTTTTGT TTTTGTCAAA CAAAATAGTA ATACAATTTG TTTTTTTTTA
    GTACAAAGAA ACTAAATAGG TCCAAATTGT TTTTTTTTTA ACNTTCANCC
    AAAAAANCCA AAATNGANGC TTTTTTCAAN AAATCNAAAT CAAAACTTTN
    GTNTTCAAGT TTTNTANTTT CACTATATNT AAAGNCCAGT TTCTGAAATT
    TAAAAAATNA TTTACCTATN TNTTACTTGG TTAACANANA AAATTCGNGC
    TGAAATACNT TCCAAGNGNG GNTANTTATG CTTNANACCA TNAATCGTTT
    GACTTAAACC ACCCCCCTGC NNAGGAA

AT1G02580.1

Model type
Protein coding
Short Description
SET domain-containing protein
Curator Summary
Encodes a putative transcription factor MEDEA (MEA) that negatively regulates seed development in the absence of fertilization. Mutations in this locus result in embryo lethality. MEA is a Polycomb group gene that is imprinted in the endosperm. The maternal allele is expressed and the paternal allele is silent. MEA is controlled by DEMETER (DME), a DNA glycosylase required to activate MEA expression, and METHYLTRANSFERASE I (MET1), which maintains CG methylation at the MEA locus. MEA is involved in the negative regulation of its own imprinted gene expression; the effect is not only allele-specific but also dynamically regulated during seed development. In the ovule, the MEA transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization
Computational Description
MEDEA (MEA); CONTAINS InterPro DOMAIN/s: SANT, DNA-binding (InterPro:IPR001005), SET domain (InterPro:IPR001214); BEST Arabidopsis thaliana protein match is: SET domain-containing protein (TAIR:AT4G02020.1); Has 4870 Blast hits to 4762 proteins in 465 species: Archae - 0; Bacteria - 401; Metazoa - 1924; Fungi - 484; Plants - 1009; Viruses - 0; Other Eukaryotes - 1052 (source: NCBI BLink).
Link
InterPro Scan - TAIR