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11-6598-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage		 Phenotype
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed yield::sterile)
stamen (PO:0009029)
  • abnormal (PATO:0000460)
    (Flowers::stamen)

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 23745260 [Map]; Strand: w
      • AT1G63990.1 - [+] show detail - sporulation 11-2
        Distance: 0; Region: intron1 (CDS)
    Flanking Genome Sequence: 11-6598-1 - 5-prime edge 
    GATTTCAGTC AGAACTACGA GATTCGACAG TGAAGAAGAC TAGTGATTCG 
    ATAAATTCAA AATTGGAAGC GAAACTTACT TCGTGAGGAA GAAGAATGTC 
    AGCGTAAGAG AGGTGTTGAT CGGAGAAGAA CNTTCATCGA TGATAGTCCT 
    GAACTTTCCT CCATTTTGAG ATTTTTTTTT CCGATTCCGC TTTTTTGAAA 
    TTTTGATTTG AATTTGATTG AGAGAGACAG AGAGGCGCGC GTCAAGTTTC 
    GGGATTTGAG ATTTGCACTG TCTTCCGTTT TGTTTATAAG AAACCGGCAT 
    GCAGTTCATG ATTTTTTTTC TCATGTGGAA CTAATNGGGN CATATATTTN 
    ATTTTATTTT TTAATTTTAT ATATAAGCCA AAAATTTAGC CTAACTACAA 
    NCCAAAATTG TAATTGTTTT GCGGTNGTAT AGTTTTAAGC TATCAATAAC 
    TATNTTTCAA TGNGGNAATC AATTGATTTT TGTAGATATT TAATTTACNC 
    AATAAGTTGA ATCGGAAAGT NCAATTTTAT ATACTCTCTT TGTTTTTTNT 
    AANNGANGTT NTAAGNTNNN ANNCCGGGNC GGGNNACCNC CCGCCCC

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 23745144 [Map]; Strand: w
      • AT1G63990.1 - [+] show detail - sporulation 11-2
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 11-6598-1 - 3-prime edge 
    CTGAAATCGA AGCTCGTTGT TACAGGCTCG TGCTAGAATC GAAGTTTCTG 
    TTCTCAATCT CCTCCGAATT TTGAACTCCC CAGATCCAGC TATCTCCGAT 
    CTCTCTCTGG TACTACTTAA TCTCGTGCTT AAGTCCGTTT CTTAGATCGC 
    GTTATAGCTT TCTCGAATT

AT1G63990.1

Model type
Protein coding
Short Description
sporulation 11-2
Curator Summary
Encodes AtSPO11-2, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. Plants homozygous for atspo11-2 exhibit a severe sterility phenotype. Both male and female meiosis are severely disrupted in the atspo11-2 mutant, and this is associated with severe defects in synapsis during the first meiotic division and reduced meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. Required for double-strand break induction.
Computational Description
sporulation 11-2 (SPO11-2); FUNCTIONS IN: DNA topoisomerase (ATP-hydrolyzing) activity, DNA binding, catalytic activity, ATP binding; INVOLVED IN: in 11 processes; LOCATED IN: chromosome; CONTAINS InterPro DOMAIN/s: Spo11/DNA topoisomerase VI, subunit A, N-terminal (InterPro:IPR013049), Spo11/DNA topoisomerase VI, subunit A (InterPro:IPR002815); BEST Arabidopsis thaliana protein match is: Spo11/DNA topoisomerase VI, subunit A protein (TAIR:AT5G02820.1); Has 890 Blast hits to 889 proteins in 304 species: Archae - 214; Bacteria - 14; Metazoa - 169; Fungi - 107; Plants - 162; Viruses - 0; Other Eukaryotes - 224 (source: NCBI BLink).
Link
InterPro Scan - TAIR