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12-0500-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage
Phenotype
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed yield::low yield)

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 7967578 [Map]; Strand: w
      • AT5G23630.1 - [+] show detail - phosphate deficiency response 2
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 12-0500-1 - 3-prime edge
    TGTGGGAGGA AAGGTAGTGG AGAAGGTTGA TTTATGTAGG AAGAAACAAT 
    TGGTGTGGCG CTTGGACGTT TGGCCATTTG CGATTCTGTA TACTGTATGG
    TTGACTACGA TCGTTCCTAG CATAGACTTC AGTGATGCNT TGCATTGCGC
    NTTGGTGGTC TTTCTGCTTT CCACATCTTA GTCTTGCTTT TCACTACGTG
    GTCCGTGGAT TTCAAGTGTT TTGTCCAGTT TAGCAAGGTA AACAGGCAGT
    CCAATGGCTG TTACCTATTT TCTTTTACAG ATGAATCTTG AGTTACTGGT
    ATTGACCGGT CCTGTGTCTG TGTCTGTTTA TTCACAGGTT AACAGTATCG
    ACCAGGCAGA TGCATGTAAA GTTACACCAG CCAAATTTTC TGGTTCTAAA
    GAAGTTGTGC CTCTTCATTT TCGTAGCCAA GTAAGTTCAT TCAANATTTT
    TTGTTTAATA GTGAAGTTGT CGCTAAAGTA GTCTCCATGA ATGGGTTTCT
    ATGTTTCTAG GTTGTTCTCG TGATATATGT AAAGTTCANT GTTTTTCCCC
    TTNACAAACC GATAATTTGN CTCTGCCATT TCTCTCTGCN TNAAATTTGA
    TGAATTAAAG CCCTATACTT TTTGAATCCT NTTTGGCNNN GGAAATGCNT
    GATTTTATNA NNACTNTTAC TTNTNAGCTT TTNANANNNT NAANATTTTN
    CTGNTTTNNA ANAANAAAA

AT5G23630.1

Model type
Protein coding
Short Description
phosphate deficiency response 2
Curator Summary
A member of the eukaryotic type V subfamily (P5) of P-type ATPase cation pumps; MIA is most similar to the human P5 ATPase ATY2(44% identity) and to Spf1p from S. cerevisiae (41% identity). Highly abundant in the endoplasmic reticulum and small vesicles of developing pollen grains and tapetum cells. T-DNA insertional mutants of MIA suffer from imbalances in cation homeostasis and exhibit a severe reduction in fertility. Mutant microspores fail to separate from tetrads and pollen grains are fragile with an abnormal morphology and altered cell wall structure. MIA is also named PDR2 and was shown to be required for proper expression of SCARECROW (SCR), a key regulator of root patterning, and for stem-cell maintenance in Pi-deprived roots.
Computational Description
phosphate deficiency response 2 (PDR2); FUNCTIONS IN: cation-transporting ATPase activity; INVOLVED IN: in 6 processes; LOCATED IN: endoplasmic reticulum, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Haloacid dehalogenase-like hydrolase (InterPro:IPR005834), ATPase, P-type, unknown pump specificity (type V) (InterPro:IPR006544), ATPase, P-type, ATPase-associated domain (InterPro:IPR008250), ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (InterPro:IPR001757), ATPase, P-type phosphorylation site (InterPro:IPR018303); BEST Arabidopsis thaliana protein match is: calcium ATPase 2 (TAIR:AT4G37640.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR