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Mutant Lines
12-0614-1

12-0614-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 3; Position: 23053335 [Map]; Strand: c
  - AT3G62290.2 - [+] show detail - ADP-ribosylation factor A1E
    Distance: 0; Region: intron5 (CDS)
  - AT3G62290.3 - [+] show detail - ADP-ribosylation factor A1E
    Distance: 0; Region: intron5 (CDS)
  - AT3G62290.1 - [+] show detail - ADP-ribosylation factor A1E
    Distance: 0; Region: intron5 (CDS)
  - AT3G62300.2 - [+] show detail - DOMAIN OF UNKNOWN FUNCTION 724 7
    Distance: -669; Region: promoter
  - AT3G62300.1 - [+] show detail - DOMAIN OF UNKNOWN FUNCTION 724 7
    Distance: -769; Region: promoter
Flanking Genome Sequence: 12-0614-1 - 5-prime edge
```
CTCCGGAGGT AGCACATGTG CTCTGTATGT ACCTGTTCAA CAAACCCCAC 
CAACGATTTA CATCAGCTTT TCATGTTACG ATATAGGTAT CTGGTAAATA 
AAGAGATTTG GTTTGGTTTT CTTTGGCTCA CCAGTGTCGT TGACGGAGAG 
AGTGAAGTCC AAGCT
```

3 prime edge [+] show sequence

Chromosome: 3; Position: 23053451 [Map]; Strand: c
- AT3G62290.2 - [+] show detail - ADP-ribosylation factor A1E
  Distance: 0; Region: exon6 (CDS)
- AT3G62290.3 - [+] show detail - ADP-ribosylation factor A1E
  Distance: 0; Region: exon6 (CDS)
- AT3G62290.1 - [+] show detail - ADP-ribosylation factor A1E
  Distance: 0; Region: exon6 (CDS)
- AT3G62300.2 - [+] show detail - DOMAIN OF UNKNOWN FUNCTION 724 7
  Distance: -553; Region: promoter
- AT3G62300.1 - [+] show detail - DOMAIN OF UNKNOWN FUNCTION 724 7
  Distance: -653; Region: promoter

CTCCGGAGAA GGGCTTTATG AGGGACTTGA CTGGCTCTCT AACAACATCG 
CAAACAAGGT ATACAACTTT ACAATTTTTG AAATCGTATT AGTAAGAAAT 
TGATTGTTAA AGACTTACTC AAATGTGTGA TTAACTTTGC AGGCNTTAGG 
AGAACAACNG TGTGGTTTGG TTTTTAATCG AGGAGAGTCT TGTCTTCTTC 
CTTCTATCTT TTGTTCTTTC GTCTCAAACT CTTATCATTT CATACTCTTT 
GTTCTTCGCT CTAATAATTT ACAAGTTTTT TTTTTNGNGG CTTTTNNCCT 
NTAATNNAAA CTNTTNTAAA AGGGGGCCCA NTTTCGGNGG ATTTNCCANT 
TTTTTTTTTT TTTTNNNNGN CCCCNANTTT TTTNGGGNTT NNNNTTTCCC 
NAANNTTTTT TTTTNNNNNG GNCCCNATTN CGNNAAAAAN CNTNAAAAAN 
NNNGGNANNA AANCCCNNNC NTTTTTNNNN GGGTTTTNNT NAAAAAAANA 
AAACCCCCCN NTTTNGNNCC CNTTTTGGNC NNNNNTTTNC NTTTNAAAAA 
AAAAACGNNT TTTNAAANNN TCCCCCNANT TTTTTTNGGG GGGGGNCCCC 
CCCNNNNAAA AANCCCCCNN CCCCCNNNNN AAAAANAAAA AAACCCNNNN 
NNAAAAAANN NAAAAAA

AT3G62290.1

Model type: Protein coding
Short Description: ADP-ribosylation factor A1E
Curator Summary: A member of ARF GTPase family. A thaliana has 21 members of this family, known to be essential for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division, cell expansion and cellulose production using antisense construct.
Computational Description: ADP-ribosylation factor A1E (ARFA1E); FUNCTIONS IN: phospholipase activator activity, GTP binding; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ADP-ribosylation factor (InterPro:IPR006688), Small GTP-binding protein (InterPro:IPR005225), Ras small GTPase, Rab type (InterPro:IPR003579), Small GTPase SAR1-type (InterPro:IPR006687), ARF/SAR superfamily (InterPro:IPR006689); BEST Arabidopsis thaliana protein match is: Ras-related small GTP-binding family protein (TAIR:AT2G47170.1); Has 15041 Blast hits to 15022 proteins in 509 species: Archae - 14; Bacteria - 51; Metazoa - 7727; Fungi - 1941; Plants - 2031; Viruses - 3; Other Eukaryotes - 3274 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G62290.2

Model type: Protein coding
Short Description: ADP-ribosylation factor A1E
Curator Summary: A member of ARF GTPase family. A thaliana has 21 members of this family, known to be essential for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division, cell expansion and cellulose production using antisense construct.
Computational Description: ADP-ribosylation factor A1E (ARFA1E); CONTAINS InterPro DOMAIN/s: ADP-ribosylation factor (InterPro:IPR006688), Small GTP-binding protein (InterPro:IPR005225), Ras small GTPase, Rab type (InterPro:IPR003579), Small GTPase SAR1-type (InterPro:IPR006687), ARF/SAR superfamily (InterPro:IPR006689); BEST Arabidopsis thaliana protein match is: Ras-related small GTP-binding family protein (TAIR:AT2G47170.1).
Link: InterPro Scan - TAIR

AT3G62290.3

Model type: Protein coding
Short Description: ADP-ribosylation factor A1E
Curator Summary: A member of ARF GTPase family. A thaliana has 21 members of this family, known to be essential for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division, cell expansion and cellulose production using antisense construct.
Computational Description: ADP-ribosylation factor A1E (ARFA1E); CONTAINS InterPro DOMAIN/s: ADP-ribosylation factor (InterPro:IPR006688), Small GTP-binding protein (InterPro:IPR005225), Ras small GTPase, Rab type (InterPro:IPR003579), Small GTPase SAR1-type (InterPro:IPR006687), ARF/SAR superfamily (InterPro:IPR006689); BEST Arabidopsis thaliana protein match is: Ras-related small GTP-binding family protein (TAIR:AT2G47170.1).
Link: InterPro Scan - TAIR

AT3G62300.1

Model type: Protein coding
Short Description: DOMAIN OF UNKNOWN FUNCTION 724 7
Curator Summary: Member of the plant-specific DUF724 protein family. Arabidopsis has 10 DUF724 proteins. Loss of function mutant has a WT phenotype
Computational Description: DOMAIN OF UNKNOWN FUNCTION 724 7 (DUF7); FUNCTIONS IN: RNA binding; LOCATED IN: nucleus; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Tudor-like, plant (InterPro:IPR014002), Agenet (InterPro:IPR008395), Protein of unknown function DUF724 (InterPro:IPR007930); BEST Arabidopsis thaliana protein match is: DOMAIN OF UNKNOWN FUNCTION 724 6 (TAIR:AT2G47230.1); Has 299 Blast hits to 227 proteins in 36 species: Archae - 0; Bacteria - 4; Metazoa - 29; Fungi - 0; Plants - 252; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G62300.2

Model type: Protein coding
Short Description: DOMAIN OF UNKNOWN FUNCTION 724 7
Curator Summary: Member of the plant-specific DUF724 protein family. Arabidopsis has 10 DUF724 proteins. Loss of function mutant has a WT phenotype
Computational Description: DOMAIN OF UNKNOWN FUNCTION 724 7 (DUF7); FUNCTIONS IN: RNA binding; LOCATED IN: nucleus; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Agenet (InterPro:IPR008395), Tudor-like, plant (InterPro:IPR014002), Protein of unknown function DUF724 (InterPro:IPR007930); BEST Arabidopsis thaliana protein match is: DOMAIN OF UNKNOWN FUNCTION 724 6 (TAIR:AT2G47230.1).
Link: InterPro Scan - TAIR