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Mutant Lines
12-0893-1

12-0893-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 1; Position: 30045060 [Map]; Strand: w
  - AT1G79860.1 - [+] show detail - RHO guanyl-nucleotide exchange factor 12
    Distance: -885; Region: promoter
  - AT1G79870.1 - [+] show detail - D-isomer specific 2-hydroxyacid dehydrogenase family protein
    Distance: 0; Region: exon1 (CDS)
  - AT1G79870.2 - [+] show detail - D-isomer specific 2-hydroxyacid dehydrogenase family protein
    Distance: 0; Region: exon1 (CDS)
Flanking Genome Sequence: 12-0893-1 - 5-prime edge
```
CCCAACCTTG AGATTGTATC CAGCTTCAGC GTCGGGCTCG ACAAGATCGA 
TTTGGGGAAA TGCAAAGAAA AAGGGATC
```

3 prime edge [+] show sequence

Chromosome: 1; Position: 30044980 [Map]; Strand: w
- AT1G79860.1 - [+] show detail - RHO guanyl-nucleotide exchange factor 12
  Distance: -805; Region: promoter
- AT1G79870.1 - [+] show detail - D-isomer specific 2-hydroxyacid dehydrogenase family protein
  Distance: 0; Region: exon1 (CDS)
- AT1G79870.2 - [+] show detail - D-isomer specific 2-hydroxyacid dehydrogenase family protein
  Distance: 0; Region: exon1 (CDS)

ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCC 
GCTGATGAGC TGAGCATCGG CGCCGGCAGA AGCATTCCCA ACGACGGCGC 
GGATGGAGTT CCGATGAGTT TCTAGGAGGA CGGATTTCTC CGGAGAAGTC 
CAGAAGCGAA GAAGGTTGAA GCGCTTCTCA AGCTCGTTCT CGAGGTAGGA 
GGACATGGGG CACATCATAA GGACTCCGAT TGATTCCATA TCTCCTTTTC 
TCTGTTTTTT ATTTTTATTT TTCTTCTGCA AATGATTGAG TCAGTGAGAG 
GTCTGTGTAT AATTTATCGG AGGTTGACGA TAAAGATGGG CCTATTTTTG 
GGCCCATAAT TGATATTATC CGCTTTTTAT GGGCCACTAA ATGACCCTTT 
TCAAGTTTTC CTCTTGGTTC CGAAATCATG ACTTATGTTT AAAGACAACA 
TTGCACGTTT GATGATATTC ATGCAAATTG TTGTAGGATT CATGAACATG 
ATACAAACAC AAGAAGACAT ACACTGTATG TGTATGGAAA CGTAGCAGAT 
TCCCCGTTGA CATTGCATGT TTAGGGAATC CAGTGACCAA GGGTGTAATC 
ACATTTTAGT ATGTTACTAT ATGATAACAT TTTATAATTT TAACGGCTCT 
CTATCTCTAA TTATCGATGT TCAAAATCCT AAATAATGAT TAGTTAAAAT 
TAAGAGCTGN GGTCTGAAAC ATGCGCCCCA CGTNCCATTT TNCAAAAAAA 
GGAAGCAAAA GAAGAAAGCT CCCAATTTTN TNAAAGCCAA ATCACTGTAC 
AATTN

AT1G79860.1

Model type: Protein coding
Short Description: RHO guanyl-nucleotide exchange factor 12
Curator Summary: Encodes a member of KPP-like gene family, homolog of KPP (kinase partner protein) gene in tomato. Also a member of the RopGEF (guanine nucleotide exchange factor) family, containing the novel PRONE domain (plant-specific Rop nucleotide exchanger), which is exclusively active towards members of the Rop subfamily. Coexpression of AtPRK2a with AtRopGEF12 resulted in isotropic pollen tube growth Growth.
Computational Description: RHO guanyl-nucleotide exchange factor 12 (ROPGEF12); CONTAINS InterPro DOMAIN/s: Rop nucleotide exchanger, PRONE (InterPro:IPR005512); BEST Arabidopsis thaliana protein match is: RHO guanyl-nucleotide exchange factor 11 (TAIR:AT1G52240.1); Has 305 Blast hits to 303 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 305; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G79870.1

Model type: Protein coding
Short Description: D-isomer specific 2-hydroxyacid dehydrogenase family protein
Computational Description: D-isomer specific 2-hydroxyacid dehydrogenase family protein; FUNCTIONS IN: in 6 functions; INVOLVED IN: metabolic process; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (InterPro:IPR006139), D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (InterPro:IPR006140), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: D-isomer specific 2-hydroxyacid dehydrogenase family protein (TAIR:AT1G12550.1); Has 29704 Blast hits to 29696 proteins in 2729 species: Archae - 468; Bacteria - 18164; Metazoa - 733; Fungi - 1180; Plants - 594; Viruses - 5; Other Eukaryotes - 8560 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G79870.2

Model type: Protein coding
Short Description: D-isomer specific 2-hydroxyacid dehydrogenase family protein
Computational Description: D-isomer specific 2-hydroxyacid dehydrogenase family protein; FUNCTIONS IN: in 6 functions; INVOLVED IN: metabolic process; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (InterPro:IPR006139), D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (InterPro:IPR006140), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: D-isomer specific 2-hydroxyacid dehydrogenase family protein (TAIR:AT1G12550.1).
Link: InterPro Scan - TAIR